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Cytotoxicity assessment of four pharmaceutical compounds on the zebra mussel (Dreissena polymorpha) haemocytes, gill and digestive gland primary cell cultures.
Chemosphere. 2011 Jun; 84(1):91-100.C

Abstract

Pharmaceutical compounds are considered the new environmental pollutants but at present few studies have evaluated their ecotoxicity on aquatic invertebrates. This study was aimed to investigate the in vitro cytotoxicity of four common drugs, namely atenolol (ATL), carbamazepine (CBZ), diclofenac (DCF) and gemfibrozil (GEM), on three different cell typologies from the zebra mussel (Dreissena polymorpha): haemocytes, gill and digestive gland cells. Results obtained by the Trypan blue exclusion test revealed that exposure to increasing concentrations (0.001; 0.01; 0.1; 1 and 10 mg L(-1)) of CBZ, DCF and GEM were able to significantly decrease the viability of each cell type, while the MTT (3(4,5-dimethyl-2thiazholyl)-2,5-diphenyl-2H-tetrazolium bromide) reduction assay highlighted only a slight reduction of mitochondrial activity of gill and digestive gland cells. Overall, DCF was the most cytotoxic drug for zebra mussel cells, followed by GEM, CBZ, while ATL has not a noteworthy toxic potential. Our preliminary results lay the groundwork for further in vitro evaluations, which will allow a better definition of the potential toxicity of these drugs.

Authors+Show Affiliations

Department of Biology, University of Milan, Via Celoria 26, 20133 Milan, Italy. marco.parolini@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21420712

Citation

Parolini, Marco, et al. "Cytotoxicity Assessment of Four Pharmaceutical Compounds On the Zebra Mussel (Dreissena Polymorpha) Haemocytes, Gill and Digestive Gland Primary Cell Cultures." Chemosphere, vol. 84, no. 1, 2011, pp. 91-100.
Parolini M, Quinn B, Binelli A, et al. Cytotoxicity assessment of four pharmaceutical compounds on the zebra mussel (Dreissena polymorpha) haemocytes, gill and digestive gland primary cell cultures. Chemosphere. 2011;84(1):91-100.
Parolini, M., Quinn, B., Binelli, A., & Provini, A. (2011). Cytotoxicity assessment of four pharmaceutical compounds on the zebra mussel (Dreissena polymorpha) haemocytes, gill and digestive gland primary cell cultures. Chemosphere, 84(1), 91-100. https://doi.org/10.1016/j.chemosphere.2011.02.049
Parolini M, et al. Cytotoxicity Assessment of Four Pharmaceutical Compounds On the Zebra Mussel (Dreissena Polymorpha) Haemocytes, Gill and Digestive Gland Primary Cell Cultures. Chemosphere. 2011;84(1):91-100. PubMed PMID: 21420712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity assessment of four pharmaceutical compounds on the zebra mussel (Dreissena polymorpha) haemocytes, gill and digestive gland primary cell cultures. AU - Parolini,Marco, AU - Quinn,Brian, AU - Binelli,Andrea, AU - Provini,Alfredo, Y1 - 2011/03/21/ PY - 2011/01/12/received PY - 2011/02/16/revised PY - 2011/02/23/accepted PY - 2011/3/23/entrez PY - 2011/3/23/pubmed PY - 2011/7/30/medline SP - 91 EP - 100 JF - Chemosphere JO - Chemosphere VL - 84 IS - 1 N2 - Pharmaceutical compounds are considered the new environmental pollutants but at present few studies have evaluated their ecotoxicity on aquatic invertebrates. This study was aimed to investigate the in vitro cytotoxicity of four common drugs, namely atenolol (ATL), carbamazepine (CBZ), diclofenac (DCF) and gemfibrozil (GEM), on three different cell typologies from the zebra mussel (Dreissena polymorpha): haemocytes, gill and digestive gland cells. Results obtained by the Trypan blue exclusion test revealed that exposure to increasing concentrations (0.001; 0.01; 0.1; 1 and 10 mg L(-1)) of CBZ, DCF and GEM were able to significantly decrease the viability of each cell type, while the MTT (3(4,5-dimethyl-2thiazholyl)-2,5-diphenyl-2H-tetrazolium bromide) reduction assay highlighted only a slight reduction of mitochondrial activity of gill and digestive gland cells. Overall, DCF was the most cytotoxic drug for zebra mussel cells, followed by GEM, CBZ, while ATL has not a noteworthy toxic potential. Our preliminary results lay the groundwork for further in vitro evaluations, which will allow a better definition of the potential toxicity of these drugs. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/21420712/Cytotoxicity_assessment_of_four_pharmaceutical_compounds_on_the_zebra_mussel__Dreissena_polymorpha__haemocytes_gill_and_digestive_gland_primary_cell_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(11)00213-X DB - PRIME DP - Unbound Medicine ER -