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Entrapment of conjunctival goblet cells by desiccation-induced cornification.
Invest Ophthalmol Vis Sci. 2011 Jun 01; 52(6):3492-9.IO

Abstract

PURPOSE

To evaluate the effects of desiccating stress on conjunctival goblet cell density and morphology and the expression of cornified envelope precursors by the ocular surface epithelia.

METHODS

Experimental dry eye (EDE) was created in C57BL/6 mice. Real-time PCR evaluated the expression of cornified envelope (CE) precursor proteins (involucrin and small proline-rich [Sprr] -1a, -1b, -2a, -2b, -2f, and -2g proteins), the cross-linking transglutaminase 1 enzyme (Tg-1) and Muc5AC mRNA transcripts by the ocular surface epithelia. Laser scanning confocal microscopy evaluated the expression of the CE precursor proteins Tg-1 and Muc5AC in cryosections. Tg-1 activity was measured by a fluorescein cadaverine assay. Muc5AC concentration was measured by ELISA.

RESULTS

Levels of involucrin; Sprr-1a, -1b, -2a, -2b, -2f, and -2g; and Tg1-1 mRNA transcripts in ocular surface tissues increased in response to desiccating stress. Expression and activity of Tg in the conjunctiva markedly increased after EDE. Desiccating stress caused progressive loss of mucin-filled goblet cells. The apical portion of the remaining conjunctival goblet cells became entrapped by adjacent stratified apical epithelia expressing increased levels of cornified envelope precursors.

CONCLUSIONS

Exposure to desiccating stress stimulates ocular surface epithelia to produce cornified envelope precursors and the tissue transglutaminase enzyme that cross-links them. This effect is accompanied by loss of mucin-filled goblet cells and entrapment of mucin contents in the remaining ones by cornifying cells that block the egress of mucin contents to the ocular surface. This mechanism may contribute to the conjunctival mucin deficiency that develops in dry eye.

Authors+Show Affiliations

Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas 77030, USA. corrales@bcm.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21421863

Citation

Corrales, Rosa Maria, et al. "Entrapment of Conjunctival Goblet Cells By Desiccation-induced Cornification." Investigative Ophthalmology & Visual Science, vol. 52, no. 6, 2011, pp. 3492-9.
Corrales RM, de Paiva CS, Li DQ, et al. Entrapment of conjunctival goblet cells by desiccation-induced cornification. Invest Ophthalmol Vis Sci. 2011;52(6):3492-9.
Corrales, R. M., de Paiva, C. S., Li, D. Q., Farley, W. J., Henriksson, J. T., Bergmanson, J. P., & Pflugfelder, S. C. (2011). Entrapment of conjunctival goblet cells by desiccation-induced cornification. Investigative Ophthalmology & Visual Science, 52(6), 3492-9. https://doi.org/10.1167/iovs.10-5782
Corrales RM, et al. Entrapment of Conjunctival Goblet Cells By Desiccation-induced Cornification. Invest Ophthalmol Vis Sci. 2011 Jun 1;52(6):3492-9. PubMed PMID: 21421863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Entrapment of conjunctival goblet cells by desiccation-induced cornification. AU - Corrales,Rosa Maria, AU - de Paiva,Cintia Sade, AU - Li,De-Quan, AU - Farley,William Johnson, AU - Henriksson,Johanna Tukler, AU - Bergmanson,Jan Per Gustav, AU - Pflugfelder,Stephen Carl, Y1 - 2011/06/01/ PY - 2011/3/23/entrez PY - 2011/3/23/pubmed PY - 2011/8/20/medline SP - 3492 EP - 9 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 52 IS - 6 N2 - PURPOSE: To evaluate the effects of desiccating stress on conjunctival goblet cell density and morphology and the expression of cornified envelope precursors by the ocular surface epithelia. METHODS: Experimental dry eye (EDE) was created in C57BL/6 mice. Real-time PCR evaluated the expression of cornified envelope (CE) precursor proteins (involucrin and small proline-rich [Sprr] -1a, -1b, -2a, -2b, -2f, and -2g proteins), the cross-linking transglutaminase 1 enzyme (Tg-1) and Muc5AC mRNA transcripts by the ocular surface epithelia. Laser scanning confocal microscopy evaluated the expression of the CE precursor proteins Tg-1 and Muc5AC in cryosections. Tg-1 activity was measured by a fluorescein cadaverine assay. Muc5AC concentration was measured by ELISA. RESULTS: Levels of involucrin; Sprr-1a, -1b, -2a, -2b, -2f, and -2g; and Tg1-1 mRNA transcripts in ocular surface tissues increased in response to desiccating stress. Expression and activity of Tg in the conjunctiva markedly increased after EDE. Desiccating stress caused progressive loss of mucin-filled goblet cells. The apical portion of the remaining conjunctival goblet cells became entrapped by adjacent stratified apical epithelia expressing increased levels of cornified envelope precursors. CONCLUSIONS: Exposure to desiccating stress stimulates ocular surface epithelia to produce cornified envelope precursors and the tissue transglutaminase enzyme that cross-links them. This effect is accompanied by loss of mucin-filled goblet cells and entrapment of mucin contents in the remaining ones by cornifying cells that block the egress of mucin contents to the ocular surface. This mechanism may contribute to the conjunctival mucin deficiency that develops in dry eye. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/21421863/Entrapment_of_conjunctival_goblet_cells_by_desiccation_induced_cornification_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.10-5782 DB - PRIME DP - Unbound Medicine ER -