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Metabolic alterations of Withania somnifera (L.) dunal fruits at different developmental stages by NMR spectroscopy.
Phytochem Anal. 2011 Nov-Dec; 22(6):492-502.PA

Abstract

INTRODUCTION

Withania somnifera (Ashwagandha) is a high-value Ayurvedic medicinal plant and an important constituent of several dietary supplements. In order to substantiate the health claims, the herb has drawn considerable scientific attention.

OBJECTIVE

The objective of the study was to investigate the alterations in primary and secondary metabolites of W. somnifera fruits during its maturity using NMR spectroscopy.

METHODOLOGY

Fruits at different stages of development from one week after fertilisation until maturity, classified in seven developmental stages, were analysed by a combined use of one- and two-dimensional NMR experiments.

RESULTS

Seventeen metabolites were characterised and quantified from non-polar and polar extracts of different fruit development stages of W. somnifera. The principal component analysis of polar metabolites at different stages could be grossly classified into three metabolic phases, viz. initial phase, developmental phase and maturation phase.

CONCLUSION

Qualitative and quantitative analysis of metabolites in W. somnifera fruits indicated specific stages when fruits can be harvested for obtaining substantial bioactive ingredients for desirable pharmacological activity. This study potentially provides a complementary tool for quality control of herbal medicinal products when W. somnifera fruits are used.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Sidhu OP
National Botanical Research Institute, Rana Pratap Marg, Lucknow 226001, India.
Annarao S
No affiliation info available
Chatterjee S
No affiliation info available
Tuli R
No affiliation info available
Roy R
No affiliation info available
Khetrapal CL
No affiliation info available

MeSH

Caffeic AcidsFruitLipidsMagnetic Resonance SpectroscopyPlant ExtractsPrincipal Component AnalysisQuality ControlSolubilitySucroseWithaniaWithanolides

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21425379

Citation

Sidhu, O P., et al. "Metabolic Alterations of Withania Somnifera (L.) Dunal Fruits at Different Developmental Stages By NMR Spectroscopy." Phytochemical Analysis : PCA, vol. 22, no. 6, 2011, pp. 492-502.
Sidhu OP, Annarao S, Chatterjee S, et al. Metabolic alterations of Withania somnifera (L.) dunal fruits at different developmental stages by NMR spectroscopy. Phytochem Anal. 2011;22(6):492-502.
Sidhu, O. P., Annarao, S., Chatterjee, S., Tuli, R., Roy, R., & Khetrapal, C. L. (2011). Metabolic alterations of Withania somnifera (L.) dunal fruits at different developmental stages by NMR spectroscopy. Phytochemical Analysis : PCA, 22(6), 492-502. https://doi.org/10.1002/pca.1307
Sidhu OP, et al. Metabolic Alterations of Withania Somnifera (L.) Dunal Fruits at Different Developmental Stages By NMR Spectroscopy. Phytochem Anal. 2011 Nov-Dec;22(6):492-502. PubMed PMID: 21425379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic alterations of Withania somnifera (L.) dunal fruits at different developmental stages by NMR spectroscopy. AU - Sidhu,O P, AU - Annarao,Sanjay, AU - Chatterjee,Sandipan, AU - Tuli,Rakesh, AU - Roy,Raja, AU - Khetrapal,C L, Y1 - 2011/03/22/ PY - 2010/04/13/received PY - 2010/10/07/revised PY - 2010/10/18/accepted PY - 2011/3/23/entrez PY - 2011/3/23/pubmed PY - 2012/2/18/medline SP - 492 EP - 502 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 22 IS - 6 N2 - INTRODUCTION: Withania somnifera (Ashwagandha) is a high-value Ayurvedic medicinal plant and an important constituent of several dietary supplements. In order to substantiate the health claims, the herb has drawn considerable scientific attention. OBJECTIVE: The objective of the study was to investigate the alterations in primary and secondary metabolites of W. somnifera fruits during its maturity using NMR spectroscopy. METHODOLOGY: Fruits at different stages of development from one week after fertilisation until maturity, classified in seven developmental stages, were analysed by a combined use of one- and two-dimensional NMR experiments. RESULTS: Seventeen metabolites were characterised and quantified from non-polar and polar extracts of different fruit development stages of W. somnifera. The principal component analysis of polar metabolites at different stages could be grossly classified into three metabolic phases, viz. initial phase, developmental phase and maturation phase. CONCLUSION: Qualitative and quantitative analysis of metabolites in W. somnifera fruits indicated specific stages when fruits can be harvested for obtaining substantial bioactive ingredients for desirable pharmacological activity. This study potentially provides a complementary tool for quality control of herbal medicinal products when W. somnifera fruits are used. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/21425379/Metabolic_alterations_of_Withania_somnifera__L___dunal_fruits_at_different_developmental_stages_by_NMR_spectroscopy_ L2 - https://doi.org/10.1002/pca.1307 DB - PRIME DP - Unbound Medicine ER -