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Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds.
Nat Prod Commun. 2011 Feb; 6(2):213-6.NP

Abstract

In this study, the antioxidant capacities of main quinone constituents of Nigella sativa seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 microg/mL and IC50 value of 2.4 microg/mL in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 microg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties.

Authors+Show Affiliations

Department of Crop Sciences and Agroforestry, Institute of Tropics and Subtropics, Czech University of Life Sciences Prague, Kamycka 129, 165 21, Prague 6-Suchdol, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21425677

Citation

Tesarova, Hana, et al. "Determination of Oxygen Radical Absorbance Capacity of Black Cumin (Nigella Sativa) Seed Quinone Compounds." Natural Product Communications, vol. 6, no. 2, 2011, pp. 213-6.
Tesarova H, Svobodova B, Kokoska L, et al. Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds. Nat Prod Commun. 2011;6(2):213-6.
Tesarova, H., Svobodova, B., Kokoska, L., Marsik, P., Pribylova, M., Landa, P., & Vadlejch, J. (2011). Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds. Natural Product Communications, 6(2), 213-6.
Tesarova H, et al. Determination of Oxygen Radical Absorbance Capacity of Black Cumin (Nigella Sativa) Seed Quinone Compounds. Nat Prod Commun. 2011;6(2):213-6. PubMed PMID: 21425677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds. AU - Tesarova,Hana, AU - Svobodova,Blanka, AU - Kokoska,Ladislav, AU - Marsik,Petr, AU - Pribylova,Marie, AU - Landa,Premysl, AU - Vadlejch,Jaroslav, PY - 2011/3/24/entrez PY - 2011/3/24/pubmed PY - 2011/4/19/medline SP - 213 EP - 6 JF - Natural product communications JO - Nat Prod Commun VL - 6 IS - 2 N2 - In this study, the antioxidant capacities of main quinone constituents of Nigella sativa seeds, namely dithymoquinone (1), thymohydroquinone (2) and thymoquinone (3), were compared using DPPH and ORAC methods. The best scavenging activity was produced by 2, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 microg/mL and IC50 value of 2.4 microg/mL in ORAC and DPPH assays, respectively. Contrastingly, 3 possessed only weak DPPH scavenging efficacy (IC50 = 170 microg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. No effect has been observed for 1. Additionally, modified protocol for synthesis of 2 has been developed with aim to enhance its availability for further studies as well as for its future potential use. Based on the results of this study, we conclude that 2 could be considered as a compound with prospective antioxidative properties. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/21425677/Determination_of_oxygen_radical_absorbance_capacity_of_black_cumin__Nigella_sativa__seed_quinone_compounds_ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -