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Attenuating effect of Acorus calamus extract in chronic constriction injury induced neuropathic pain in rats: an evidence of anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory effects.
BMC Complement Altern Med. 2011 Mar 22; 11:24.BC

Abstract

BACKGROUND

Acorus calamus (family: Araceae), is an indigenous plant, traditionally it is used as an ingredient of various cocktail preparations and for the management of severe inflammatory disorders in Indian system of medicine. Present study investigated the attenuating role of Acorus calamus plant extract in chronic constriction injury (CCI) of sciatic nerve induced peripheral neuropathy in rats.

METHODS

Hot plate, plantar, Randall Selitto, Von Frey Hair, pin prick, acetone drop, photoactometer and rota-rod tests were performed to assess degree of thermal, radiant, mechanical, chemical sensation, spontaneous motor activity and motor co-ordination changes respectively, at different time intervals i.e., day 0, 1, 3, 6, 9, 12, 15, 18 and 21. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after 21st day to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus calamus (HAE-AC, 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered from the day of surgery for 14 days.

RESULTS

CCI of sciatic nerve significantly induced thermal, radiant, mechanical hyperalgesia and thermal, chemical, tactile allodynia, along with increase in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover significant histological changes were also observed. HAE-AC attenuated CCI induced development of painful behavioural, biochemical and histological changes in a dose dependent manner similar to that of pregabalin serving as positive control.

CONCLUSIONS

Acorus calamus prevented CCI induced neuropathy which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions.

Authors+Show Affiliations

Department of pharmaceutical sciences and drug research, Punjabi university, Patiala-147002, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21426568

Citation

Muthuraman, Arunachalam, and Nirmal Singh. "Attenuating Effect of Acorus Calamus Extract in Chronic Constriction Injury Induced Neuropathic Pain in Rats: an Evidence of Anti-oxidative, Anti-inflammatory, Neuroprotective and Calcium Inhibitory Effects." BMC Complementary and Alternative Medicine, vol. 11, 2011, p. 24.
Muthuraman A, Singh N. Attenuating effect of Acorus calamus extract in chronic constriction injury induced neuropathic pain in rats: an evidence of anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory effects. BMC Complement Altern Med. 2011;11:24.
Muthuraman, A., & Singh, N. (2011). Attenuating effect of Acorus calamus extract in chronic constriction injury induced neuropathic pain in rats: an evidence of anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory effects. BMC Complementary and Alternative Medicine, 11, 24. https://doi.org/10.1186/1472-6882-11-24
Muthuraman A, Singh N. Attenuating Effect of Acorus Calamus Extract in Chronic Constriction Injury Induced Neuropathic Pain in Rats: an Evidence of Anti-oxidative, Anti-inflammatory, Neuroprotective and Calcium Inhibitory Effects. BMC Complement Altern Med. 2011 Mar 22;11:24. PubMed PMID: 21426568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuating effect of Acorus calamus extract in chronic constriction injury induced neuropathic pain in rats: an evidence of anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory effects. AU - Muthuraman,Arunachalam, AU - Singh,Nirmal, Y1 - 2011/03/22/ PY - 2010/10/26/received PY - 2011/03/22/accepted PY - 2011/3/24/entrez PY - 2011/3/24/pubmed PY - 2011/8/27/medline SP - 24 EP - 24 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 11 N2 - BACKGROUND: Acorus calamus (family: Araceae), is an indigenous plant, traditionally it is used as an ingredient of various cocktail preparations and for the management of severe inflammatory disorders in Indian system of medicine. Present study investigated the attenuating role of Acorus calamus plant extract in chronic constriction injury (CCI) of sciatic nerve induced peripheral neuropathy in rats. METHODS: Hot plate, plantar, Randall Selitto, Von Frey Hair, pin prick, acetone drop, photoactometer and rota-rod tests were performed to assess degree of thermal, radiant, mechanical, chemical sensation, spontaneous motor activity and motor co-ordination changes respectively, at different time intervals i.e., day 0, 1, 3, 6, 9, 12, 15, 18 and 21. Tissue myeloperoxidase, superoxide anion and total calcium levels were determined after 21st day to assess biochemical alterations. Histopathological evaluations were also performed. Hydroalcoholic extract of Acorus calamus (HAE-AC, 100 and 200 mg/kg, p.o.) and pregabalin (10 mg/kg, p.o.) were administered from the day of surgery for 14 days. RESULTS: CCI of sciatic nerve significantly induced thermal, radiant, mechanical hyperalgesia and thermal, chemical, tactile allodynia, along with increase in the levels of superoxide anion, total calcium and myeloperoxidase activity. Moreover significant histological changes were also observed. HAE-AC attenuated CCI induced development of painful behavioural, biochemical and histological changes in a dose dependent manner similar to that of pregabalin serving as positive control. CONCLUSIONS: Acorus calamus prevented CCI induced neuropathy which may be attributed to its multiple actions including anti-oxidative, anti-inflammatory, neuroprotective and calcium inhibitory actions. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/21426568/Attenuating_effect_of_Acorus_calamus_extract_in_chronic_constriction_injury_induced_neuropathic_pain_in_rats:_an_evidence_of_anti_oxidative_anti_inflammatory_neuroprotective_and_calcium_inhibitory_effects_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-11-24 DB - PRIME DP - Unbound Medicine ER -