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Prepartum nutrient intake alters palmitate metabolism by liver slices from peripartal dairy cows.
J Dairy Sci. 2011 Apr; 94(4):1928-40.JD

Abstract

We determined the effects of day relative to parturition and prepartum plane of nutrition on hepatic partitioning of palmitate metabolism to CO2, acid-soluble products (ASP), and esterified products (EP). Multiparous Holsteins (n=74) were fed different amounts of nutrients during the dry period in a 3 (far-off period diet)×2 (close-up period diet) factorial arrangement. During the far-off period (d -60 to -25) cows received a low-energy control diet fed ad libitum (100NRC) to meet National Research Council (NRC) requirements, a moderate-energy diet fed ad libitum to exceed NRC recommendations for net energy of lactation (NEL) by >50% (150NRC), or the same diet fed at restricted intake to provide 80% of NEL requirements (80NRC). During the close-up period (d -24 until parturition), cows were fed a diet for ad libitum intake to meet NRC recommendations or in restricted amounts to provide 80% of calculated NEL requirements. After parturition, all cows had ad libitum access to a lactation diet. Liver slices from biopsies on d -30, -14, 1, 14, and 28 relative to parturition were used to determine conversion of [1-(14)C] palmitate to CO2, ASP, and EP. Across diets, oxidation of palmitate to CO2 was decreased postpartum, whereas oxidation to ASP was increased at d 1 postpartum compared with other times. Conversion of palmitate to EP increased markedly postpartum, with the greatest rates at d 1 postpartum. Conversion of palmitate to CO2 and ASP on d 1 postpartum was lower and the proportion of palmitate metabolism as EP was greater for cows fed 150NRC than for those fed 100NRC or 80NRC. Hepatic triacylglycerol concentration at d 1 postpartum was greatest for cows fed 150NRC. Palmitate metabolism did not differ between close-up diets. Hepatic triacylglycerol was negatively correlated with tissue metabolism of palmitate to CO2 and ASP but positively correlated with metabolism to EP. Hepatic triacylglycerol was highly correlated with NEFA concentrations on the day of calving and d 1 postpartum but not with NEFA prepartum. In contrast, plasma BHBA postpartum was not correlated with hepatic palmitate metabolism by liver slices but was highly correlated with NEFA concentration prepartum. Excessive energy intake during the far-off dry period decreased hepatic palmitate oxidation and shifted palmitate metabolism toward greater esterification, consistent with greater hepatic triacylglycerol accumulation postpartum.

Authors+Show Affiliations

Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21426984

Citation

Litherland, N B., et al. "Prepartum Nutrient Intake Alters Palmitate Metabolism By Liver Slices From Peripartal Dairy Cows." Journal of Dairy Science, vol. 94, no. 4, 2011, pp. 1928-40.
Litherland NB, Dann HM, Drackley JK. Prepartum nutrient intake alters palmitate metabolism by liver slices from peripartal dairy cows. J Dairy Sci. 2011;94(4):1928-40.
Litherland, N. B., Dann, H. M., & Drackley, J. K. (2011). Prepartum nutrient intake alters palmitate metabolism by liver slices from peripartal dairy cows. Journal of Dairy Science, 94(4), 1928-40. https://doi.org/10.3168/jds.2010-3220
Litherland NB, Dann HM, Drackley JK. Prepartum Nutrient Intake Alters Palmitate Metabolism By Liver Slices From Peripartal Dairy Cows. J Dairy Sci. 2011;94(4):1928-40. PubMed PMID: 21426984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prepartum nutrient intake alters palmitate metabolism by liver slices from peripartal dairy cows. AU - Litherland,N B, AU - Dann,H M, AU - Drackley,J K, PY - 2010/03/02/received PY - 2010/12/21/accepted PY - 2011/3/24/entrez PY - 2011/3/24/pubmed PY - 2011/6/28/medline SP - 1928 EP - 40 JF - Journal of dairy science JO - J Dairy Sci VL - 94 IS - 4 N2 - We determined the effects of day relative to parturition and prepartum plane of nutrition on hepatic partitioning of palmitate metabolism to CO2, acid-soluble products (ASP), and esterified products (EP). Multiparous Holsteins (n=74) were fed different amounts of nutrients during the dry period in a 3 (far-off period diet)×2 (close-up period diet) factorial arrangement. During the far-off period (d -60 to -25) cows received a low-energy control diet fed ad libitum (100NRC) to meet National Research Council (NRC) requirements, a moderate-energy diet fed ad libitum to exceed NRC recommendations for net energy of lactation (NEL) by >50% (150NRC), or the same diet fed at restricted intake to provide 80% of NEL requirements (80NRC). During the close-up period (d -24 until parturition), cows were fed a diet for ad libitum intake to meet NRC recommendations or in restricted amounts to provide 80% of calculated NEL requirements. After parturition, all cows had ad libitum access to a lactation diet. Liver slices from biopsies on d -30, -14, 1, 14, and 28 relative to parturition were used to determine conversion of [1-(14)C] palmitate to CO2, ASP, and EP. Across diets, oxidation of palmitate to CO2 was decreased postpartum, whereas oxidation to ASP was increased at d 1 postpartum compared with other times. Conversion of palmitate to EP increased markedly postpartum, with the greatest rates at d 1 postpartum. Conversion of palmitate to CO2 and ASP on d 1 postpartum was lower and the proportion of palmitate metabolism as EP was greater for cows fed 150NRC than for those fed 100NRC or 80NRC. Hepatic triacylglycerol concentration at d 1 postpartum was greatest for cows fed 150NRC. Palmitate metabolism did not differ between close-up diets. Hepatic triacylglycerol was negatively correlated with tissue metabolism of palmitate to CO2 and ASP but positively correlated with metabolism to EP. Hepatic triacylglycerol was highly correlated with NEFA concentrations on the day of calving and d 1 postpartum but not with NEFA prepartum. In contrast, plasma BHBA postpartum was not correlated with hepatic palmitate metabolism by liver slices but was highly correlated with NEFA concentration prepartum. Excessive energy intake during the far-off dry period decreased hepatic palmitate oxidation and shifted palmitate metabolism toward greater esterification, consistent with greater hepatic triacylglycerol accumulation postpartum. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/21426984/Prepartum_nutrient_intake_alters_palmitate_metabolism_by_liver_slices_from_peripartal_dairy_cows_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(11)00163-9 DB - PRIME DP - Unbound Medicine ER -