Tags

Type your tag names separated by a space and hit enter

Molecularly imprinted solid-phase extraction for determination of tilmicosin in feed using high performance liquid chromatography.
Anal Chim Acta. 2011 Apr 01; 690(2):269-74.AC

Abstract

A simple, sensitive and reproducible molecularly imprinted solid-phase extraction (MISPE) coupled with high performance liquid chromatographic method was developed for monitoring tilmicosin in feeds. The polymers were prepared using tylosin as mimic template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linking monomer, and chloroform as a solvent by bulk polymerization. Under the optimum MISPE conditions, the novel polymer sorbent can selectively extract and enrich tilmicosin from variety of feeds. The MISPE cartridge was better than non-imprinted, C(18) and HLB cartridges in terms of both recovery and precision. Mean recoveries of tilmicosin from five kinds of feeds spiked at 1, 10 and 50 mg kg(-1) ranged from 76.9% to 95.6%, with intra-day and inter-day relative standard deviation less than 7.6%. The linearity was ranged from 1.0 to 100 mg L(-1) for matrix standard solution (r=0.9990). The limit of detection was approximately 0.35 mg kg(-1) and the limit of quantification was approximately 0.98 mg kg(-1). There was cleaner chromatogram by using MISPE than C(18) and HLB SPE.

Authors+Show Affiliations

Department of Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, PC 510642, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21435486

Citation

Zheng, Yaqiu, et al. "Molecularly Imprinted Solid-phase Extraction for Determination of Tilmicosin in Feed Using High Performance Liquid Chromatography." Analytica Chimica Acta, vol. 690, no. 2, 2011, pp. 269-74.
Zheng Y, Liu Y, Guo H, et al. Molecularly imprinted solid-phase extraction for determination of tilmicosin in feed using high performance liquid chromatography. Anal Chim Acta. 2011;690(2):269-74.
Zheng, Y., Liu, Y., Guo, H., He, L., Fang, B., & Zeng, Z. (2011). Molecularly imprinted solid-phase extraction for determination of tilmicosin in feed using high performance liquid chromatography. Analytica Chimica Acta, 690(2), 269-74. https://doi.org/10.1016/j.aca.2011.02.029
Zheng Y, et al. Molecularly Imprinted Solid-phase Extraction for Determination of Tilmicosin in Feed Using High Performance Liquid Chromatography. Anal Chim Acta. 2011 Apr 1;690(2):269-74. PubMed PMID: 21435486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid-phase extraction for determination of tilmicosin in feed using high performance liquid chromatography. AU - Zheng,Yaqiu, AU - Liu,Yahong, AU - Guo,Hongbin, AU - He,Limin, AU - Fang,Binghu, AU - Zeng,Zhenling, Y1 - 2011/02/18/ PY - 2010/10/07/received PY - 2011/02/09/revised PY - 2011/02/13/accepted PY - 2011/3/26/entrez PY - 2011/3/26/pubmed PY - 2011/7/8/medline SP - 269 EP - 74 JF - Analytica chimica acta JO - Anal Chim Acta VL - 690 IS - 2 N2 - A simple, sensitive and reproducible molecularly imprinted solid-phase extraction (MISPE) coupled with high performance liquid chromatographic method was developed for monitoring tilmicosin in feeds. The polymers were prepared using tylosin as mimic template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linking monomer, and chloroform as a solvent by bulk polymerization. Under the optimum MISPE conditions, the novel polymer sorbent can selectively extract and enrich tilmicosin from variety of feeds. The MISPE cartridge was better than non-imprinted, C(18) and HLB cartridges in terms of both recovery and precision. Mean recoveries of tilmicosin from five kinds of feeds spiked at 1, 10 and 50 mg kg(-1) ranged from 76.9% to 95.6%, with intra-day and inter-day relative standard deviation less than 7.6%. The linearity was ranged from 1.0 to 100 mg L(-1) for matrix standard solution (r=0.9990). The limit of detection was approximately 0.35 mg kg(-1) and the limit of quantification was approximately 0.98 mg kg(-1). There was cleaner chromatogram by using MISPE than C(18) and HLB SPE. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/21435486/Molecularly_imprinted_solid_phase_extraction_for_determination_of_tilmicosin_in_feed_using_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)00254-6 DB - PRIME DP - Unbound Medicine ER -