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A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat.
Theor Appl Genet. 2011 Jun; 123(1):159-67.TA

Abstract

Stem rust (Puccinia graminis f. sp. tritici Eriks. & E. Henn.) (the causal agent of wheat stem rust) race Ug99 (also designated TTKSK) and its derivatives have defeated several important stem rust resistance genes widely used in wheat (Triticum aestivum L.) production, rendering much of the worldwide wheat acreage susceptible. In order to identify new resistance sources, a large collection of wheat relatives and genetic stocks maintained at the Wheat Genetic and Genomic Resources Center was screened. The results revealed that most accessions of the diploid relative Dasypyrum villosum (L.) Candargy were highly resistant. The screening of a set of wheat-D. villosum chromosome addition lines revealed that the wheat-D. villosum disomic addition line DA6V#3 was moderately resistant to race Ug99. The objective of the present study was to produce and characterize compensating wheat-D. villosum whole arm Robertsonian translocations (RobTs) involving chromosomes 6D of wheat and 6V#3 of D. villosum through the mechanism of centric breakage-fusion. Seven 6V#3-specific EST-STS markers were developed for screening F(2) progeny derived from plants double-monosomic for chromosomes 6D and 6V#3. Surprisingly, although 6D was the target chromosome, all recovered RobTs involved chromosome 6A implying a novel mechanism for the origin of RobTs. Homozygous translocations (T6AS·6V#3L and T6AL·6V#3S) with good plant vigor and full fertility were selected from F(3) families. A stem rust resistance gene was mapped to the long arm 6V#3L in T6AS·6V#3L and was designated as Sr52. Sr52 is temperature-sensitive and is most effective at 16°C, partially effective at 24°C, and ineffective at 28°C. The T6AS·6V#3L stock is a new source of resistance to Ug99, is cytogenetically stable, and may be useful in wheat improvement.

Authors+Show Affiliations

USDA-ARS, Northern Crop Science Lab, Fargo, ND 58102-2765, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21437597

Citation

Qi, L L., et al. "A Novel Robertsonian Translocation Event Leads to Transfer of a Stem Rust Resistance Gene (Sr52) Effective Against Race Ug99 From Dasypyrum Villosum Into Bread Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 123, no. 1, 2011, pp. 159-67.
Qi LL, Pumphrey MO, Friebe B, et al. A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet. 2011;123(1):159-67.
Qi, L. L., Pumphrey, M. O., Friebe, B., Zhang, P., Qian, C., Bowden, R. L., Rouse, M. N., Jin, Y., & Gill, B. S. (2011). A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 123(1), 159-67. https://doi.org/10.1007/s00122-011-1574-z
Qi LL, et al. A Novel Robertsonian Translocation Event Leads to Transfer of a Stem Rust Resistance Gene (Sr52) Effective Against Race Ug99 From Dasypyrum Villosum Into Bread Wheat. Theor Appl Genet. 2011;123(1):159-67. PubMed PMID: 21437597.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. AU - Qi,L L, AU - Pumphrey,M O, AU - Friebe,Bernd, AU - Zhang,P, AU - Qian,C, AU - Bowden,R L, AU - Rouse,M N, AU - Jin,Y, AU - Gill,B S, Y1 - 2011/03/25/ PY - 2010/12/14/received PY - 2011/03/11/accepted PY - 2011/3/26/entrez PY - 2011/3/26/pubmed PY - 2011/9/9/medline SP - 159 EP - 67 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 123 IS - 1 N2 - Stem rust (Puccinia graminis f. sp. tritici Eriks. & E. Henn.) (the causal agent of wheat stem rust) race Ug99 (also designated TTKSK) and its derivatives have defeated several important stem rust resistance genes widely used in wheat (Triticum aestivum L.) production, rendering much of the worldwide wheat acreage susceptible. In order to identify new resistance sources, a large collection of wheat relatives and genetic stocks maintained at the Wheat Genetic and Genomic Resources Center was screened. The results revealed that most accessions of the diploid relative Dasypyrum villosum (L.) Candargy were highly resistant. The screening of a set of wheat-D. villosum chromosome addition lines revealed that the wheat-D. villosum disomic addition line DA6V#3 was moderately resistant to race Ug99. The objective of the present study was to produce and characterize compensating wheat-D. villosum whole arm Robertsonian translocations (RobTs) involving chromosomes 6D of wheat and 6V#3 of D. villosum through the mechanism of centric breakage-fusion. Seven 6V#3-specific EST-STS markers were developed for screening F(2) progeny derived from plants double-monosomic for chromosomes 6D and 6V#3. Surprisingly, although 6D was the target chromosome, all recovered RobTs involved chromosome 6A implying a novel mechanism for the origin of RobTs. Homozygous translocations (T6AS·6V#3L and T6AL·6V#3S) with good plant vigor and full fertility were selected from F(3) families. A stem rust resistance gene was mapped to the long arm 6V#3L in T6AS·6V#3L and was designated as Sr52. Sr52 is temperature-sensitive and is most effective at 16°C, partially effective at 24°C, and ineffective at 28°C. The T6AS·6V#3L stock is a new source of resistance to Ug99, is cytogenetically stable, and may be useful in wheat improvement. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/21437597/A_novel_Robertsonian_translocation_event_leads_to_transfer_of_a_stem_rust_resistance_gene__Sr52__effective_against_race_Ug99_from_Dasypyrum_villosum_into_bread_wheat_ L2 - https://dx.doi.org/10.1007/s00122-011-1574-z DB - PRIME DP - Unbound Medicine ER -