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Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration.
BMC Plant Biol. 2011 Mar 28; 11:55.BP

Abstract

BACKGROUND

Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene.

RESULTS

In the present work, an apple spermidine synthase gene (MdSPDS1) was introduced into sweet orange (Citrus sinensis Osbeck 'Anliucheng') via Agrobacterium-mediated transformation of embryogenic calluses. Two transgenic lines (TG4 and TG9) varied in the transgene expression and cellular endogenous polyamine contents. Pinprick inoculation demonstrated that the transgenic lines were less susceptible to Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus canker, than the wild type plants (WT). In addition, our data showed that upon Xac attack TG9 had significantly higher free spermine (Spm) and polyamine oxidase (PAO) activity when compared with the WT, concurrent with an apparent hypersensitive response and the accumulation of more H₂O₂. Pretreatment of TG9 leaves with guazatine acetate, an inhibitor of PAO, repressed PAO activity and reduced H₂O₂ accumulation, leading to more conspicuous disease symptoms than the controls when both were challenged with Xac. Moreover, mRNA levels of most of the defense-related genes involved in synthesis of pathogenesis-related protein and jasmonic acid were upregulated in TG9 than in the WT regardless of Xac infection.

CONCLUSION

Our results demonstrated that overexpression of the MdSPDS1 gene prominently lowered the sensitivity of the transgenic plants to canker. This may be, at least partially, correlated with the generation of more H₂O₂ due to increased production of polyamines and enhanced PAO-mediated catabolism, triggering hypersensitive response or activation of defense-related genes.

Authors+Show Affiliations

Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China. liujihong@mail.hzau.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21439092

Citation

Fu, Xing-Zheng, et al. "Ectopic Expression of MdSPDS1 in Sweet Orange (Citrus Sinensis Osbeck) Reduces Canker Susceptibility: Involvement of H₂O₂ Production and Transcriptional Alteration." BMC Plant Biology, vol. 11, 2011, p. 55.
Fu XZ, Chen CW, Wang Y, et al. Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration. BMC Plant Biol. 2011;11:55.
Fu, X. Z., Chen, C. W., Wang, Y., Liu, J. H., & Moriguchi, T. (2011). Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration. BMC Plant Biology, 11, 55. https://doi.org/10.1186/1471-2229-11-55
Fu XZ, et al. Ectopic Expression of MdSPDS1 in Sweet Orange (Citrus Sinensis Osbeck) Reduces Canker Susceptibility: Involvement of H₂O₂ Production and Transcriptional Alteration. BMC Plant Biol. 2011 Mar 28;11:55. PubMed PMID: 21439092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ectopic expression of MdSPDS1 in sweet orange (Citrus sinensis Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration. AU - Fu,Xing-Zheng, AU - Chen,Chuan-Wu, AU - Wang,Yin, AU - Liu,Ji-Hong, AU - Moriguchi,Takaya, Y1 - 2011/03/28/ PY - 2010/12/16/received PY - 2011/03/28/accepted PY - 2011/3/29/entrez PY - 2011/3/29/pubmed PY - 2011/7/23/medline SP - 55 EP - 55 JF - BMC plant biology JO - BMC Plant Biol VL - 11 N2 - BACKGROUND: Enormous work has shown that polyamines are involved in a variety of physiological processes, but information is scarce on the potential of modifying disease response through genetic transformation of a polyamine biosynthetic gene. RESULTS: In the present work, an apple spermidine synthase gene (MdSPDS1) was introduced into sweet orange (Citrus sinensis Osbeck 'Anliucheng') via Agrobacterium-mediated transformation of embryogenic calluses. Two transgenic lines (TG4 and TG9) varied in the transgene expression and cellular endogenous polyamine contents. Pinprick inoculation demonstrated that the transgenic lines were less susceptible to Xanthomonas axonopodis pv. citri (Xac), the causal agent of citrus canker, than the wild type plants (WT). In addition, our data showed that upon Xac attack TG9 had significantly higher free spermine (Spm) and polyamine oxidase (PAO) activity when compared with the WT, concurrent with an apparent hypersensitive response and the accumulation of more H₂O₂. Pretreatment of TG9 leaves with guazatine acetate, an inhibitor of PAO, repressed PAO activity and reduced H₂O₂ accumulation, leading to more conspicuous disease symptoms than the controls when both were challenged with Xac. Moreover, mRNA levels of most of the defense-related genes involved in synthesis of pathogenesis-related protein and jasmonic acid were upregulated in TG9 than in the WT regardless of Xac infection. CONCLUSION: Our results demonstrated that overexpression of the MdSPDS1 gene prominently lowered the sensitivity of the transgenic plants to canker. This may be, at least partially, correlated with the generation of more H₂O₂ due to increased production of polyamines and enhanced PAO-mediated catabolism, triggering hypersensitive response or activation of defense-related genes. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/21439092/Ectopic_expression_of_MdSPDS1_in_sweet_orange__Citrus_sinensis_Osbeck__reduces_canker_susceptibility:_involvement_of_H₂O₂_production_and_transcriptional_alteration_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-11-55 DB - PRIME DP - Unbound Medicine ER -