Tags

Type your tag names separated by a space and hit enter

Simultaneous determination of HFBA-derivatized amphetamines and ketamines in urine by gas chromatography-mass spectrometry.
J Anal Toxicol. 2011 Apr; 35(3):162-9.JA

Abstract

To facilitate the analysis of targeted drugs under high sample volume testing environment, an extraction, derivatization and gas chromatographic-mass spectrometric analysis method was developed for simultaneously determination of amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), ketamine, and norketamine in urine. This method utilized solid-phase extraction in conjunction with derivatization using heptafluorobutyric anhydride (HFBA) as the derivatization reagent. Using a 1-mL sample, the limits of quantitation achieved for the analysis of AMP, MAMP, MDA, MDMA, MDEA, ketamine, and norketamine were 25, 15, 60, 60, 70, 25, and 30 ng/mL, respectively. Upper limits of quantitation were 8000 ng/mL for all amphetamines and 6000 ng/mL for ketamine and norketamine. Except for dehydronorketamine (DHNK), within-day and between-day precisions (as expressed in CV%) for quality control samples were ≤ 3.1% and ≤ 4.95%, respectively. Except DHNK, the within-day accuracy ranged between 96.0% and 110.7% and the between-day accuracy ranged between 96.9% and 108.7%. A group of 107 urine samples previously determined to contain the target analytes were analyzed by this new approach. Quantitative data produced by both methods agreed well. With this new approach, we were able to use a single analytical protocol to conduct the confirmation test for samples that preliminarily tested positive (by immunoassay) for amphetamines, ketamine, or both.

Authors+Show Affiliations

Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21439152

Citation

Lee, Hei Hwa, et al. "Simultaneous Determination of HFBA-derivatized Amphetamines and Ketamines in Urine By Gas Chromatography-mass Spectrometry." Journal of Analytical Toxicology, vol. 35, no. 3, 2011, pp. 162-9.
Lee HH, Lee JF, Lin SY, et al. Simultaneous determination of HFBA-derivatized amphetamines and ketamines in urine by gas chromatography-mass spectrometry. J Anal Toxicol. 2011;35(3):162-9.
Lee, H. H., Lee, J. F., Lin, S. Y., Chen, P. H., & Chen, B. H. (2011). Simultaneous determination of HFBA-derivatized amphetamines and ketamines in urine by gas chromatography-mass spectrometry. Journal of Analytical Toxicology, 35(3), 162-9.
Lee HH, et al. Simultaneous Determination of HFBA-derivatized Amphetamines and Ketamines in Urine By Gas Chromatography-mass Spectrometry. J Anal Toxicol. 2011;35(3):162-9. PubMed PMID: 21439152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of HFBA-derivatized amphetamines and ketamines in urine by gas chromatography-mass spectrometry. AU - Lee,Hei Hwa, AU - Lee,Jong Feng, AU - Lin,Sin Yu, AU - Chen,Ping Ho, AU - Chen,Bai Hsiun, PY - 2011/3/29/entrez PY - 2011/3/29/pubmed PY - 2011/6/15/medline SP - 162 EP - 9 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 35 IS - 3 N2 - To facilitate the analysis of targeted drugs under high sample volume testing environment, an extraction, derivatization and gas chromatographic-mass spectrometric analysis method was developed for simultaneously determination of amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), ketamine, and norketamine in urine. This method utilized solid-phase extraction in conjunction with derivatization using heptafluorobutyric anhydride (HFBA) as the derivatization reagent. Using a 1-mL sample, the limits of quantitation achieved for the analysis of AMP, MAMP, MDA, MDMA, MDEA, ketamine, and norketamine were 25, 15, 60, 60, 70, 25, and 30 ng/mL, respectively. Upper limits of quantitation were 8000 ng/mL for all amphetamines and 6000 ng/mL for ketamine and norketamine. Except for dehydronorketamine (DHNK), within-day and between-day precisions (as expressed in CV%) for quality control samples were ≤ 3.1% and ≤ 4.95%, respectively. Except DHNK, the within-day accuracy ranged between 96.0% and 110.7% and the between-day accuracy ranged between 96.9% and 108.7%. A group of 107 urine samples previously determined to contain the target analytes were analyzed by this new approach. Quantitative data produced by both methods agreed well. With this new approach, we were able to use a single analytical protocol to conduct the confirmation test for samples that preliminarily tested positive (by immunoassay) for amphetamines, ketamine, or both. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/21439152/Simultaneous_determination_of_HFBA_derivatized_amphetamines_and_ketamines_in_urine_by_gas_chromatography_mass_spectrometry_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/anatox/35.3.162 DB - PRIME DP - Unbound Medicine ER -