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Measurement of carbon flux through the MEP pathway for isoprenoid synthesis by (31)P-NMR spectroscopy after specific inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate reductase. Effect of light and temperature.
Plant Cell Environ. 2011 Aug; 34(8):1241-7.PC

Abstract

The methylerythritol 4-phosphate (MEP) and the mevalonate pathways are the unique synthesis routes for the precursors of all isoprenoids. An original mean to measure the carbon flux through the MEP pathway in plants is proposed by using cadmium as a total short-term inhibitor of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (MEcDP) reductase (GcpE) and measuring the accumulation rate of its substrate MEcDP by (31) P-NMR spectroscopy. The MEP pathway metabolic flux was determined in spinach (Spinacia oleracea), pea (Pisum sativum), Oregon grape (Mahonia aquifolium) and boxwood (Buxus sempervirens) leaves. In spinach, flux values were compared with the synthesis rate of major isoprenoids. The flux increases with light intensity (fourfold in the 200-1200 µmol m(-2) s(-1) PPFR range) and temperature (sevenfold in the 25-37 °C range). The relationship with the light and the temperature dependency of isoprenoid production downstream of the MEP pathway is discussed.

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Authors+Show Affiliations

Mongélard G
CEA, IRTSV, Laboratoire de Physiologie Cellulaire Végétale, UMR 5168, F-38054 Grenoble, France.
Seemann M
No affiliation info available
Boisson AM
No affiliation info available
Rohmer M
No affiliation info available
Bligny R
No affiliation info available
Rivasseau C
No affiliation info available

MeSH

BuxusCadmiumCarbon CycleEnzyme InhibitorsErythritolLightMagnetic Resonance SpectroscopyMahoniaMetabolic Networks and PathwaysMevalonic AcidOxidoreductasesPeasPhosphorus RadioisotopesPlantsSpinacia oleraceaTemperatureTerpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21443577

Citation

Mongélard, Gaëlle, et al. "Measurement of Carbon Flux Through the MEP Pathway for Isoprenoid Synthesis By (31)P-NMR Spectroscopy After Specific Inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate Reductase. Effect of Light and Temperature." Plant, Cell & Environment, vol. 34, no. 8, 2011, pp. 1241-7.
Mongélard G, Seemann M, Boisson AM, et al. Measurement of carbon flux through the MEP pathway for isoprenoid synthesis by (31)P-NMR spectroscopy after specific inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate reductase. Effect of light and temperature. Plant Cell Environ. 2011;34(8):1241-7.
Mongélard, G., Seemann, M., Boisson, A. M., Rohmer, M., Bligny, R., & Rivasseau, C. (2011). Measurement of carbon flux through the MEP pathway for isoprenoid synthesis by (31)P-NMR spectroscopy after specific inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate reductase. Effect of light and temperature. Plant, Cell & Environment, 34(8), 1241-7. https://doi.org/10.1111/j.1365-3040.2011.02322.x
Mongélard G, et al. Measurement of Carbon Flux Through the MEP Pathway for Isoprenoid Synthesis By (31)P-NMR Spectroscopy After Specific Inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate Reductase. Effect of Light and Temperature. Plant Cell Environ. 2011;34(8):1241-7. PubMed PMID: 21443577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of carbon flux through the MEP pathway for isoprenoid synthesis by (31)P-NMR spectroscopy after specific inhibition of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate reductase. Effect of light and temperature. AU - Mongélard,Gaëlle, AU - Seemann,Myriam, AU - Boisson,Anne-Marie, AU - Rohmer,Michel, AU - Bligny,Richard, AU - Rivasseau,Corinne, Y1 - 2011/04/26/ PY - 2011/3/30/entrez PY - 2011/3/30/pubmed PY - 2011/12/13/medline SP - 1241 EP - 7 JF - Plant, cell & environment JO - Plant Cell Environ VL - 34 IS - 8 N2 - The methylerythritol 4-phosphate (MEP) and the mevalonate pathways are the unique synthesis routes for the precursors of all isoprenoids. An original mean to measure the carbon flux through the MEP pathway in plants is proposed by using cadmium as a total short-term inhibitor of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (MEcDP) reductase (GcpE) and measuring the accumulation rate of its substrate MEcDP by (31) P-NMR spectroscopy. The MEP pathway metabolic flux was determined in spinach (Spinacia oleracea), pea (Pisum sativum), Oregon grape (Mahonia aquifolium) and boxwood (Buxus sempervirens) leaves. In spinach, flux values were compared with the synthesis rate of major isoprenoids. The flux increases with light intensity (fourfold in the 200-1200 µmol m(-2) s(-1) PPFR range) and temperature (sevenfold in the 25-37 °C range). The relationship with the light and the temperature dependency of isoprenoid production downstream of the MEP pathway is discussed. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/21443577/Measurement_of_carbon_flux_through_the_MEP_pathway_for_isoprenoid_synthesis_by__31_P_NMR_spectroscopy_after_specific_inhibition_of_2_C_methyl_d_erythritol_24_cyclodiphosphate_reductase__Effect_of_light_and_temperature_ L2 - https://doi.org/10.1111/j.1365-3040.2011.02322.x DB - PRIME DP - Unbound Medicine ER -