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Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flight-mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15; 879(13-14):938-44.JC

Abstract

Plant hormones play crucial roles in plant growth and development. However, up to date, identification and quantification of acidic plant hormones with trace amount in complicated plant matrix is still a challenge. In current study, we developed a high sensitive assay for the determination of acidic plant hormones in rice by combining capillary electrophoresis and electrospray ionization-time of flight-mass spectrometry (CE-ESI-TOF-MS). To improve the detection sensitivity of acidic plant hormones, 3-bromoactonyltrimethylammonium bromide (BTA) was synthesized as a new mass probe, which can react efficiently with acidic plant hormones in acetonitrile containing triethylamine (TEA). The positively charged BTA-derivatives were separated by CE using amino-coated capillary, which provided a reversed electroosmotic flow (EOF) at low pH, as well as reduced the adsorption of BTA-derivatives on the inner wall of capillary. Using the CE-ESI-TOF-MS method developed in current study, 15 acidic plant hormones, including 10 gibberellins (GAs), were identified and quantified with good linearities from 1.3 to 850 ng/mL with linear coefficient R(2) values of >0.99. The limits of detection (LODs) were in the range of 0.34-4.59 ng/mL. Recoveries of compounds from spiked beverage samples ranged from 84.6 to 112.2%. And a good reproducibility was obtained by evaluating the intra and inter-day precisions with relative standard deviations (RSDs) less than 6.7 and 9.9%, respectively.

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Authors+Show Affiliations

Chen ML
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
Huang YQ
No affiliation info available
Liu JQ
No affiliation info available
Yuan BF
No affiliation info available
Feng YQ
No affiliation info available

MeSH

AdsorptionElectrophoresis, CapillaryFormatesHydrogen-Ion ConcentrationMass SpectrometryOryzaPlant Growth RegulatorsQuaternary Ammonium CompoundsRegression AnalysisReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21444253

Citation

Chen, Ming-Luan, et al. "Highly Sensitive Profiling Assay of Acidic Plant Hormones Using a Novel Mass Probe By Capillary Electrophoresis-time of Flight-mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 879, no. 13-14, 2011, pp. 938-44.
Chen ML, Huang YQ, Liu JQ, et al. Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flight-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879(13-14):938-44.
Chen, M. L., Huang, Y. Q., Liu, J. Q., Yuan, B. F., & Feng, Y. Q. (2011). Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flight-mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 879(13-14), 938-44. https://doi.org/10.1016/j.jchromb.2011.03.003
Chen ML, et al. Highly Sensitive Profiling Assay of Acidic Plant Hormones Using a Novel Mass Probe By Capillary Electrophoresis-time of Flight-mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15;879(13-14):938-44. PubMed PMID: 21444253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive profiling assay of acidic plant hormones using a novel mass probe by capillary electrophoresis-time of flight-mass spectrometry. AU - Chen,Ming-Luan, AU - Huang,Yun-Qing, AU - Liu,Jia-Qi, AU - Yuan,Bi-Feng, AU - Feng,Yu-Qi, Y1 - 2011/03/10/ PY - 2010/12/17/received PY - 2011/02/26/revised PY - 2011/03/02/accepted PY - 2011/3/30/entrez PY - 2011/3/30/pubmed PY - 2011/8/4/medline SP - 938 EP - 44 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 13-14 N2 - Plant hormones play crucial roles in plant growth and development. However, up to date, identification and quantification of acidic plant hormones with trace amount in complicated plant matrix is still a challenge. In current study, we developed a high sensitive assay for the determination of acidic plant hormones in rice by combining capillary electrophoresis and electrospray ionization-time of flight-mass spectrometry (CE-ESI-TOF-MS). To improve the detection sensitivity of acidic plant hormones, 3-bromoactonyltrimethylammonium bromide (BTA) was synthesized as a new mass probe, which can react efficiently with acidic plant hormones in acetonitrile containing triethylamine (TEA). The positively charged BTA-derivatives were separated by CE using amino-coated capillary, which provided a reversed electroosmotic flow (EOF) at low pH, as well as reduced the adsorption of BTA-derivatives on the inner wall of capillary. Using the CE-ESI-TOF-MS method developed in current study, 15 acidic plant hormones, including 10 gibberellins (GAs), were identified and quantified with good linearities from 1.3 to 850 ng/mL with linear coefficient R(2) values of >0.99. The limits of detection (LODs) were in the range of 0.34-4.59 ng/mL. Recoveries of compounds from spiked beverage samples ranged from 84.6 to 112.2%. And a good reproducibility was obtained by evaluating the intra and inter-day precisions with relative standard deviations (RSDs) less than 6.7 and 9.9%, respectively. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/21444253/Highly_sensitive_profiling_assay_of_acidic_plant_hormones_using_a_novel_mass_probe_by_capillary_electrophoresis_time_of_flight_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00149-8 DB - PRIME DP - Unbound Medicine ER -
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