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Quantitation of branched-chain amino acids in ascites by capillary electrophoresis with light-emitting diode-induced fluorescence detection.
Electrophoresis. 2011 Apr; 32(9):1080-3.E

Abstract

Branched-chain amino acids (BCAAs) are one of the important biomarkers for monitoring liver disease such as hepatitis or hepatoma. In this communication, we present the determination of the concentrations of BCAA in ascites by CE light-emitted diode-induced fluorescence (LEDIF) using 1.5% m/v poly(ethylene oxide) (average M(v) : ~8 000 000 g/mol) that was prepared in 10 mM sodium tetraborate solution (pH 9.3). Naphthalene-2,3-dicarboxaldehyde was used to derivatize 15 amino acids (AAs) to form naphthalene-2,3-dicarboxaldehyde (NDA)-AA derivatives prior to CE analysis. The separation of 15 NDA-AA derivatives was accomplished within 15 min, with RSD values of <5.8% (within-day) and 7.4% (between-days) with respect to their migration times. The limits of detection for the tested BCAAs ranged from 10.6 to 10.9 nM. We determined the concentrations of three BCAAs--leucine, isoleucine and valine--in ascites by applying a standard addition method, with recovery percentages ranging from 93.9 to 111%. The results obtained from this CE-LEDIF method is in good agreement with those by a gold standard method using an AA analyzer. We have found that the concentrations of the three BCAAs in ascites obtained from patients suffering from liver diseases were lower than those from healthy individuals. Our approach is highly efficient, sensitive, and cost-effective, which holds great potential for the diagnosis of liver diseases.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chang PL
Department of Chemistry, Tunghai University, Taichung, Taiwan. poling@thu.edu.tw
Chiu TC
No affiliation info available
Wang TE
No affiliation info available
Hu KC
No affiliation info available
Tsai YH
No affiliation info available
Hu CC
No affiliation info available
Bair MJ
No affiliation info available
Chang HT
No affiliation info available

MeSH

Amino AcidsAscitic FluidElectrophoresis, CapillaryHumansLinear ModelsLiver DiseasesNaphthalenesPolyethylene GlycolsReproducibility of ResultsSensitivity and SpecificitySpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21449064

Citation

Chang, Po-Ling, et al. "Quantitation of Branched-chain Amino Acids in Ascites By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection." Electrophoresis, vol. 32, no. 9, 2011, pp. 1080-3.
Chang PL, Chiu TC, Wang TE, et al. Quantitation of branched-chain amino acids in ascites by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Electrophoresis. 2011;32(9):1080-3.
Chang, P. L., Chiu, T. C., Wang, T. E., Hu, K. C., Tsai, Y. H., Hu, C. C., Bair, M. J., & Chang, H. T. (2011). Quantitation of branched-chain amino acids in ascites by capillary electrophoresis with light-emitting diode-induced fluorescence detection. Electrophoresis, 32(9), 1080-3. https://doi.org/10.1002/elps.201000445
Chang PL, et al. Quantitation of Branched-chain Amino Acids in Ascites By Capillary Electrophoresis With Light-emitting Diode-induced Fluorescence Detection. Electrophoresis. 2011;32(9):1080-3. PubMed PMID: 21449064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of branched-chain amino acids in ascites by capillary electrophoresis with light-emitting diode-induced fluorescence detection. AU - Chang,Po-Ling, AU - Chiu,Tai-Chia, AU - Wang,Tsang-En, AU - Hu,Kuang-Chun, AU - Tsai,Yu-Hsien, AU - Hu,Cho-Chun, AU - Bair,Ming-Jong, AU - Chang,Huan-Tsung, Y1 - 2011/03/30/ PY - 2010/09/03/received PY - 2010/11/20/revised PY - 2010/11/22/accepted PY - 2011/3/31/entrez PY - 2011/3/31/pubmed PY - 2011/8/4/medline SP - 1080 EP - 3 JF - Electrophoresis JO - Electrophoresis VL - 32 IS - 9 N2 - Branched-chain amino acids (BCAAs) are one of the important biomarkers for monitoring liver disease such as hepatitis or hepatoma. In this communication, we present the determination of the concentrations of BCAA in ascites by CE light-emitted diode-induced fluorescence (LEDIF) using 1.5% m/v poly(ethylene oxide) (average M(v) : ~8 000 000 g/mol) that was prepared in 10 mM sodium tetraborate solution (pH 9.3). Naphthalene-2,3-dicarboxaldehyde was used to derivatize 15 amino acids (AAs) to form naphthalene-2,3-dicarboxaldehyde (NDA)-AA derivatives prior to CE analysis. The separation of 15 NDA-AA derivatives was accomplished within 15 min, with RSD values of <5.8% (within-day) and 7.4% (between-days) with respect to their migration times. The limits of detection for the tested BCAAs ranged from 10.6 to 10.9 nM. We determined the concentrations of three BCAAs--leucine, isoleucine and valine--in ascites by applying a standard addition method, with recovery percentages ranging from 93.9 to 111%. The results obtained from this CE-LEDIF method is in good agreement with those by a gold standard method using an AA analyzer. We have found that the concentrations of the three BCAAs in ascites obtained from patients suffering from liver diseases were lower than those from healthy individuals. Our approach is highly efficient, sensitive, and cost-effective, which holds great potential for the diagnosis of liver diseases. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/21449064/Quantitation_of_branched_chain_amino_acids_in_ascites_by_capillary_electrophoresis_with_light_emitting_diode_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.201000445 DB - PRIME DP - Unbound Medicine ER -