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The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.
EMBO J. 1990 Nov; 9(11):3667-73.EJ

Abstract

The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.

Authors+Show Affiliations

Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2145151

Citation

Maroney, P A., et al. "The Nematode Spliced Leader RNA Participates in Trans-splicing as an Sm SnRNP." The EMBO Journal, vol. 9, no. 11, 1990, pp. 3667-73.
Maroney PA, Hannon GJ, Denker JA, et al. The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP. EMBO J. 1990;9(11):3667-73.
Maroney, P. A., Hannon, G. J., Denker, J. A., & Nilsen, T. W. (1990). The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP. The EMBO Journal, 9(11), 3667-73.
Maroney PA, et al. The Nematode Spliced Leader RNA Participates in Trans-splicing as an Sm SnRNP. EMBO J. 1990;9(11):3667-73. PubMed PMID: 2145151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP. AU - Maroney,P A, AU - Hannon,G J, AU - Denker,J A, AU - Nilsen,T W, PY - 1990/11/1/pubmed PY - 1990/11/1/medline PY - 1990/11/1/entrez SP - 3667 EP - 73 JF - The EMBO journal JO - EMBO J. VL - 9 IS - 11 N2 - The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/2145151/The_nematode_spliced_leader_RNA_participates_in_trans_splicing_as_an_Sm_snRNP_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1990&volume=9&issue=11&spage=3667 DB - PRIME DP - Unbound Medicine ER -