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Structural features of polyacylated anthocyanins using matrix-assisted laser desorption/ionization and electrospray ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2011 Apr 30; 25(8):1051-60.RC

Abstract

In our continuing studies to isolate water-soluble vacuolar pigments, we expect to elucidate more structural details using mass spectrometry (MS). Because of its sensitivity, only a small amount of pigment extracted from natural plants is required for MS measurement. Nuclear magnetic resonance is also a useful spectroscopic method for structural determination. In this study, two soft ionization techniques, electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), on time-of-flight (TOF) mass spectrometers, were used to analyze five polyacylated anthocyanins with more than two aromatic acid molecules in the side chains. ESI is advantageous for the detection of individual molecular ions, while MALDI is essential for the detection of characteristic fragment ions originating from the anthocyanidin. Although 2,5-dihydroxybenzoic acid (DHBA) is an effective matrix in MALDI-TOFMS to obtain informative fragment ions of polyacylated anthocyanins, α-cyano-4-hydroxycinnamic acid (CHCA) is the preferred matrix for the identification of aglycones. In particular, in measurements of polyacylated anthocyanins with two acylated glycoside chains, fragment ions originating from anthocyanidin can only be observed in MALDI-TOFMS using CHCA as the matrix.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kasai HF
Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo, 142-8501, Japan. kasai@hoshi.ac.jp
Saito N
No affiliation info available
Honda T
No affiliation info available

MeSH

AcylationAnthocyaninsBenzopyransCaffeic AcidsCoumaric AcidsGlucosidesSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21452382

Citation

Kasai, Hiroko F., et al. "Structural Features of Polyacylated Anthocyanins Using Matrix-assisted Laser Desorption/ionization and Electrospray Ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 25, no. 8, 2011, pp. 1051-60.
Kasai HF, Saito N, Honda T. Structural features of polyacylated anthocyanins using matrix-assisted laser desorption/ionization and electrospray ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2011;25(8):1051-60.
Kasai, H. F., Saito, N., & Honda, T. (2011). Structural features of polyacylated anthocyanins using matrix-assisted laser desorption/ionization and electrospray ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 25(8), 1051-60. https://doi.org/10.1002/rcm.4956
Kasai HF, Saito N, Honda T. Structural Features of Polyacylated Anthocyanins Using Matrix-assisted Laser Desorption/ionization and Electrospray Ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2011 Apr 30;25(8):1051-60. PubMed PMID: 21452382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural features of polyacylated anthocyanins using matrix-assisted laser desorption/ionization and electrospray ionization time-of-flight mass spectrometry. AU - Kasai,Hiroko F, AU - Saito,Norio, AU - Honda,Toshio, Y1 - 2011/03/29/ PY - 2010/05/27/received PY - 2011/01/07/revised PY - 2011/01/22/accepted PY - 2011/4/1/entrez PY - 2011/4/1/pubmed PY - 2011/7/12/medline SP - 1051 EP - 60 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 25 IS - 8 N2 - In our continuing studies to isolate water-soluble vacuolar pigments, we expect to elucidate more structural details using mass spectrometry (MS). Because of its sensitivity, only a small amount of pigment extracted from natural plants is required for MS measurement. Nuclear magnetic resonance is also a useful spectroscopic method for structural determination. In this study, two soft ionization techniques, electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), on time-of-flight (TOF) mass spectrometers, were used to analyze five polyacylated anthocyanins with more than two aromatic acid molecules in the side chains. ESI is advantageous for the detection of individual molecular ions, while MALDI is essential for the detection of characteristic fragment ions originating from the anthocyanidin. Although 2,5-dihydroxybenzoic acid (DHBA) is an effective matrix in MALDI-TOFMS to obtain informative fragment ions of polyacylated anthocyanins, α-cyano-4-hydroxycinnamic acid (CHCA) is the preferred matrix for the identification of aglycones. In particular, in measurements of polyacylated anthocyanins with two acylated glycoside chains, fragment ions originating from anthocyanidin can only be observed in MALDI-TOFMS using CHCA as the matrix. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/21452382/Structural_features_of_polyacylated_anthocyanins_using_matrix_assisted_laser_desorption/ionization_and_electrospray_ionization_time_of_flight_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.4956 DB - PRIME DP - Unbound Medicine ER -
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