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Inhibition of HCV 3a core gene through Silymarin and its fractions.
Virol J. 2011 Apr 01; 8:153.VJ

Abstract

Hepatitis C is a major health problem affecting 270 million individuals in world including Pakistan. Current treatment regimen, interferon alpha and ribavirin only cure half of patients due to side effects and high cost.

RESULTS

In the present study Silybum marianum (Milk thistle) seeds were collected, extracted and analyzed against HCV 3a core gene by transiently transfecting the liver cells with HCV core plasmid. Our results demonstrated that Silymarin (SM) dose dependently inhibit the expression or function of HCV core gene at a non toxic concentration while the GAPDH remained constant. To identify the active ingredient, SM was fractioned by thin layer chromatography (TLC), column chromatography and HPLC. Purified fractions were tested for HCV core gene and western blotting results showed that two factions of SM (S1 and S2) inhibit HCV 3a core expression or function in liver cells

CONCLUSION

Our results suggest SM and its fractions (S1 and S2) inhibit HCV core gene of 3a genotype and combination of SM and its fractions with interferon will be a better option to treat HCV infection.

Authors+Show Affiliations

Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan. usmancemb@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21453551

Citation

Ashfaq, Usman Ali, et al. "Inhibition of HCV 3a Core Gene Through Silymarin and Its Fractions." Virology Journal, vol. 8, 2011, p. 153.
Ashfaq UA, Javed T, Rehman S, et al. Inhibition of HCV 3a core gene through Silymarin and its fractions. Virol J. 2011;8:153.
Ashfaq, U. A., Javed, T., Rehman, S., Nawaz, Z., & Riazuddin, S. (2011). Inhibition of HCV 3a core gene through Silymarin and its fractions. Virology Journal, 8, 153. https://doi.org/10.1186/1743-422X-8-153
Ashfaq UA, et al. Inhibition of HCV 3a Core Gene Through Silymarin and Its Fractions. Virol J. 2011 Apr 1;8:153. PubMed PMID: 21453551.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of HCV 3a core gene through Silymarin and its fractions. AU - Ashfaq,Usman Ali, AU - Javed,Tariq, AU - Rehman,Sidra, AU - Nawaz,Zafar, AU - Riazuddin,Sheikh, Y1 - 2011/04/01/ PY - 2011/02/09/received PY - 2011/04/01/accepted PY - 2011/4/2/entrez PY - 2011/4/2/pubmed PY - 2011/8/9/medline SP - 153 EP - 153 JF - Virology journal JO - Virol. J. VL - 8 N2 - UNLABELLED: Hepatitis C is a major health problem affecting 270 million individuals in world including Pakistan. Current treatment regimen, interferon alpha and ribavirin only cure half of patients due to side effects and high cost. RESULTS: In the present study Silybum marianum (Milk thistle) seeds were collected, extracted and analyzed against HCV 3a core gene by transiently transfecting the liver cells with HCV core plasmid. Our results demonstrated that Silymarin (SM) dose dependently inhibit the expression or function of HCV core gene at a non toxic concentration while the GAPDH remained constant. To identify the active ingredient, SM was fractioned by thin layer chromatography (TLC), column chromatography and HPLC. Purified fractions were tested for HCV core gene and western blotting results showed that two factions of SM (S1 and S2) inhibit HCV 3a core expression or function in liver cells CONCLUSION: Our results suggest SM and its fractions (S1 and S2) inhibit HCV core gene of 3a genotype and combination of SM and its fractions with interferon will be a better option to treat HCV infection. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/21453551/Inhibition_of_HCV_3a_core_gene_through_Silymarin_and_its_fractions_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-153 DB - PRIME DP - Unbound Medicine ER -