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CXCR2 antagonists block the N-Ac-PGP-induced neutrophil influx in the airways of mice, but not the production of the chemokine CXCL1.
Eur J Pharmacol. 2011 Oct 15; 668(3):443-9.EJ

Abstract

Neutrophils are innate immune cells in chronic inflammatory diseases including chronic obstructive pulmonary disease (COPD) and can be attracted to the site of inflammation via the collagen breakdown product N-acetyl Proline-Glycine-Proline (N-Ac-PGP). To elucidate whether CXCR2 is involved in N-Ac-PGP-induced neutrophil migration and activation, studies using specific antagonists were performed in vivo. N-Ac-PGP and keratinocyte cell-derived chemokine (KC; CXCL1) were administered in C57Bl/6 mice via oropharyngeal aspiration. Intraperitoneal applications of CXCR2 antagonist SB225002 or SB332235 were administered 1h prior and 1h after oropharyngeal aspiration. Six hours after oropharyngeal aspiration mice were sacrificed. Neutrophil counts and CXCL1 levels were determined in bronchoalveolar lavage fluid, myleoperoxidase (MPO) levels were measured in lung tissue homogenates and an immunohistological staining for neutrophils was performed on lung tissue. N-Ac-PGP and CXCL1 induced a neutrophil influx in the bronchoalveolar lavage fluid and lung tissue, which was also reflected by increased MPO levels in lung tissue. The N-Ac-PGP- and CXCL1-induced neutrophil influx and the increased pulmonary tissue MPO levels were inhibited by the CXCR2 antagonists SB225002 and SB332235. Moreover, N-Ac-PGP administration enhanced the CXCL1 levels in bronchoalveolar lavage fluid, which could not be attenuated by both CXCR2 antagonists. In conclusion, neutrophil migration induced by N-Ac-PGP is mediated via direct CXCR2 interaction. The N-Ac-PGP-induced release of CXCL1 is independent of CXCR2. Related to the maximal effect of CXCL1, N-Ac-PGP is more potent at inducing neutrophil migration in the pulmonary tissue than into the bronchoalveolar lavage fluid, or N-ac-PGP may be more potent at inducing MPO levels in the lung tissue.

Authors+Show Affiliations

Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands. s.braber@uu.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21458445

Citation

Braber, Saskia, et al. "CXCR2 Antagonists Block the N-Ac-PGP-induced Neutrophil Influx in the Airways of Mice, but Not the Production of the Chemokine CXCL1." European Journal of Pharmacology, vol. 668, no. 3, 2011, pp. 443-9.
Braber S, Overbeek SA, Koelink PJ, et al. CXCR2 antagonists block the N-Ac-PGP-induced neutrophil influx in the airways of mice, but not the production of the chemokine CXCL1. Eur J Pharmacol. 2011;668(3):443-9.
Braber, S., Overbeek, S. A., Koelink, P. J., Henricks, P. A., Zaman, G. J., Garssen, J., Kraneveld, A. D., & Folkerts, G. (2011). CXCR2 antagonists block the N-Ac-PGP-induced neutrophil influx in the airways of mice, but not the production of the chemokine CXCL1. European Journal of Pharmacology, 668(3), 443-9. https://doi.org/10.1016/j.ejphar.2011.03.025
Braber S, et al. CXCR2 Antagonists Block the N-Ac-PGP-induced Neutrophil Influx in the Airways of Mice, but Not the Production of the Chemokine CXCL1. Eur J Pharmacol. 2011 Oct 15;668(3):443-9. PubMed PMID: 21458445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CXCR2 antagonists block the N-Ac-PGP-induced neutrophil influx in the airways of mice, but not the production of the chemokine CXCL1. AU - Braber,Saskia, AU - Overbeek,Saskia A, AU - Koelink,Pim J, AU - Henricks,Paul A J, AU - Zaman,Guido J R, AU - Garssen,Johan, AU - Kraneveld,Aletta D, AU - Folkerts,Gert, Y1 - 2011/03/31/ PY - 2010/12/20/received PY - 2011/02/04/revised PY - 2011/03/08/accepted PY - 2011/4/5/entrez PY - 2011/4/5/pubmed PY - 2012/1/20/medline SP - 443 EP - 9 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 668 IS - 3 N2 - Neutrophils are innate immune cells in chronic inflammatory diseases including chronic obstructive pulmonary disease (COPD) and can be attracted to the site of inflammation via the collagen breakdown product N-acetyl Proline-Glycine-Proline (N-Ac-PGP). To elucidate whether CXCR2 is involved in N-Ac-PGP-induced neutrophil migration and activation, studies using specific antagonists were performed in vivo. N-Ac-PGP and keratinocyte cell-derived chemokine (KC; CXCL1) were administered in C57Bl/6 mice via oropharyngeal aspiration. Intraperitoneal applications of CXCR2 antagonist SB225002 or SB332235 were administered 1h prior and 1h after oropharyngeal aspiration. Six hours after oropharyngeal aspiration mice were sacrificed. Neutrophil counts and CXCL1 levels were determined in bronchoalveolar lavage fluid, myleoperoxidase (MPO) levels were measured in lung tissue homogenates and an immunohistological staining for neutrophils was performed on lung tissue. N-Ac-PGP and CXCL1 induced a neutrophil influx in the bronchoalveolar lavage fluid and lung tissue, which was also reflected by increased MPO levels in lung tissue. The N-Ac-PGP- and CXCL1-induced neutrophil influx and the increased pulmonary tissue MPO levels were inhibited by the CXCR2 antagonists SB225002 and SB332235. Moreover, N-Ac-PGP administration enhanced the CXCL1 levels in bronchoalveolar lavage fluid, which could not be attenuated by both CXCR2 antagonists. In conclusion, neutrophil migration induced by N-Ac-PGP is mediated via direct CXCR2 interaction. The N-Ac-PGP-induced release of CXCL1 is independent of CXCR2. Related to the maximal effect of CXCL1, N-Ac-PGP is more potent at inducing neutrophil migration in the pulmonary tissue than into the bronchoalveolar lavage fluid, or N-ac-PGP may be more potent at inducing MPO levels in the lung tissue. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21458445/CXCR2_antagonists_block_the_N_Ac_PGP_induced_neutrophil_influx_in_the_airways_of_mice_but_not_the_production_of_the_chemokine_CXCL1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)00337-2 DB - PRIME DP - Unbound Medicine ER -