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Brain histological changes in young mice submitted to diets with different ratios of n-6/n-3 polyunsaturated fatty acids during maternal pregnancy and lactation.
Clin Nutr. 2011 Oct; 30(5):659-67.CN

Abstract

N-3 polyunsaturated fatty acids (n-3 PUFAs) are essential for brain development and function, but the appropriate quantity of dietary n-3 PUFAs and ratio of n-6/n-3 PUFAs have not been clearly determined. In this study, we investigated the effects of different dietary ratios of n-6/n-3 PUFAs on the brain structural development in mice and the expression of associated transcription factors. C57 BL/6J mice were fed with one of two categories of n-3 PUFA-containing diets (a flaxseed oil diet and a flaxseed/fish oil mixed diet) or an n-3 PUFA-deficient diet. For each of the n-3 PUFA diets, flaxseed oil or flaxseed/fish oil was combined with other oils to yield three different n-6/n-3 ratios, which ranged from 15.7:1 to 1.6:1. The feeding regimens began two months before mouse conception and continued throughout lactation for new pups. As compared with the n-3 PUFA-deficient diet, both the flaxseed oil n-3 PUFA diets and the flaxseed/fish oil n-3 PUFA diets significantly increased the expression levels of brain neuron-specific enolase, glial fibrillary acidic protein and myelin basic protein, somewhat dose-dependently, in new pup mice at 21 d and 42 d of age. The expression of PPAR-γ in the brains of pup mice was increased only at 7 d of age with the n-3 PUFA diet, and no changes in the expression of PPAR-α and PPAR-β were found among all the diet groups. These results suggest that the higher intake amount of n-3 PUFAs with a low ratio of n-6/n-3 PUFAs at about 1-2:1, supplied during both maternal pregnancy and lactation, may be more beneficial for early brain development, and PPAR-γ may act in one of the pathways by which n-3 PUFAs promote early brain development.

Authors+Show Affiliations

Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, No. 56 Nan-li-shi Road, Beijing 100045, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21459495

Citation

Tian, Chunyu, et al. "Brain Histological Changes in Young Mice Submitted to Diets With Different Ratios of N-6/n-3 Polyunsaturated Fatty Acids During Maternal Pregnancy and Lactation." Clinical Nutrition (Edinburgh, Scotland), vol. 30, no. 5, 2011, pp. 659-67.
Tian C, Fan C, Liu X, et al. Brain histological changes in young mice submitted to diets with different ratios of n-6/n-3 polyunsaturated fatty acids during maternal pregnancy and lactation. Clin Nutr. 2011;30(5):659-67.
Tian, C., Fan, C., Liu, X., Xu, F., & Qi, K. (2011). Brain histological changes in young mice submitted to diets with different ratios of n-6/n-3 polyunsaturated fatty acids during maternal pregnancy and lactation. Clinical Nutrition (Edinburgh, Scotland), 30(5), 659-67. https://doi.org/10.1016/j.clnu.2011.03.002
Tian C, et al. Brain Histological Changes in Young Mice Submitted to Diets With Different Ratios of N-6/n-3 Polyunsaturated Fatty Acids During Maternal Pregnancy and Lactation. Clin Nutr. 2011;30(5):659-67. PubMed PMID: 21459495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain histological changes in young mice submitted to diets with different ratios of n-6/n-3 polyunsaturated fatty acids during maternal pregnancy and lactation. AU - Tian,Chunyu, AU - Fan,Chaonan, AU - Liu,Xinli, AU - Xu,Feng, AU - Qi,Kemin, Y1 - 2011/04/02/ PY - 2010/10/20/received PY - 2011/02/24/revised PY - 2011/03/10/accepted PY - 2011/4/5/entrez PY - 2011/4/5/pubmed PY - 2012/2/24/medline SP - 659 EP - 67 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 30 IS - 5 N2 - N-3 polyunsaturated fatty acids (n-3 PUFAs) are essential for brain development and function, but the appropriate quantity of dietary n-3 PUFAs and ratio of n-6/n-3 PUFAs have not been clearly determined. In this study, we investigated the effects of different dietary ratios of n-6/n-3 PUFAs on the brain structural development in mice and the expression of associated transcription factors. C57 BL/6J mice were fed with one of two categories of n-3 PUFA-containing diets (a flaxseed oil diet and a flaxseed/fish oil mixed diet) or an n-3 PUFA-deficient diet. For each of the n-3 PUFA diets, flaxseed oil or flaxseed/fish oil was combined with other oils to yield three different n-6/n-3 ratios, which ranged from 15.7:1 to 1.6:1. The feeding regimens began two months before mouse conception and continued throughout lactation for new pups. As compared with the n-3 PUFA-deficient diet, both the flaxseed oil n-3 PUFA diets and the flaxseed/fish oil n-3 PUFA diets significantly increased the expression levels of brain neuron-specific enolase, glial fibrillary acidic protein and myelin basic protein, somewhat dose-dependently, in new pup mice at 21 d and 42 d of age. The expression of PPAR-γ in the brains of pup mice was increased only at 7 d of age with the n-3 PUFA diet, and no changes in the expression of PPAR-α and PPAR-β were found among all the diet groups. These results suggest that the higher intake amount of n-3 PUFAs with a low ratio of n-6/n-3 PUFAs at about 1-2:1, supplied during both maternal pregnancy and lactation, may be more beneficial for early brain development, and PPAR-γ may act in one of the pathways by which n-3 PUFAs promote early brain development. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/21459495/Brain_histological_changes_in_young_mice_submitted_to_diets_with_different_ratios_of_n_6/n_3_polyunsaturated_fatty_acids_during_maternal_pregnancy_and_lactation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(11)00054-9 DB - PRIME DP - Unbound Medicine ER -