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Generation of Osr1 conditional mutant mice.
Genesis. 2011 May; 49(5):419-22.G

Abstract

The Odd-skipped related 1 (Osr1) gene encodes a zinc finger protein homologous to the Drosophila Odd-skipped transcription factor. During mouse embryogenesis, Osr1 is expressed in multiple tissues, including the developing heart, kidney, limb, lung, and craniofacial structures. Although characterization of targeted mutant mice has revealed essential roles for Osr1 in heart and kidney development, the embryonic lethality of the Osr1 null mice has hindered investigation of its role in many other developmental processes. We report here the generation of conditional mutant mice containing two loxP sites flanking exon 2 of the Osr1 gene. Mice homozygous for this targeted Osr1(fneo) allele are normal and fertile. Crossing the Osr1(fneo/fneo) mice with the EIIa-Cre transgenic mice resulted in Cre-mediated deletion of the loxP-flanked Exon2 in the germ line and mice homozygous for this deletion recapitulated the Osr1 null mutant phenotypes. The Osr1(fneo) conditional mice will be valuable for tissue-specific analysis of the roles of Osr1 in embryonic and postnatal developmental processes. genesis 49:419-422, 2011.

Authors+Show Affiliations

Center for Oral Biology and Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21462293

Citation

Lan, Yu, et al. "Generation of Osr1 Conditional Mutant Mice." Genesis (New York, N.Y. : 2000), vol. 49, no. 5, 2011, pp. 419-22.
Lan Y, Liu H, Ovitt CE, et al. Generation of Osr1 conditional mutant mice. Genesis. 2011;49(5):419-22.
Lan, Y., Liu, H., Ovitt, C. E., & Jiang, R. (2011). Generation of Osr1 conditional mutant mice. Genesis (New York, N.Y. : 2000), 49(5), 419-22. https://doi.org/10.1002/dvg.20734
Lan Y, et al. Generation of Osr1 Conditional Mutant Mice. Genesis. 2011;49(5):419-22. PubMed PMID: 21462293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of Osr1 conditional mutant mice. AU - Lan,Yu, AU - Liu,Han, AU - Ovitt,Catherine E, AU - Jiang,Rulang, Y1 - 2011/04/02/ PY - 2010/12/10/received PY - 2011/01/16/revised PY - 2011/02/04/accepted PY - 2011/4/5/entrez PY - 2011/4/5/pubmed PY - 2011/10/1/medline SP - 419 EP - 22 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 49 IS - 5 N2 - The Odd-skipped related 1 (Osr1) gene encodes a zinc finger protein homologous to the Drosophila Odd-skipped transcription factor. During mouse embryogenesis, Osr1 is expressed in multiple tissues, including the developing heart, kidney, limb, lung, and craniofacial structures. Although characterization of targeted mutant mice has revealed essential roles for Osr1 in heart and kidney development, the embryonic lethality of the Osr1 null mice has hindered investigation of its role in many other developmental processes. We report here the generation of conditional mutant mice containing two loxP sites flanking exon 2 of the Osr1 gene. Mice homozygous for this targeted Osr1(fneo) allele are normal and fertile. Crossing the Osr1(fneo/fneo) mice with the EIIa-Cre transgenic mice resulted in Cre-mediated deletion of the loxP-flanked Exon2 in the germ line and mice homozygous for this deletion recapitulated the Osr1 null mutant phenotypes. The Osr1(fneo) conditional mice will be valuable for tissue-specific analysis of the roles of Osr1 in embryonic and postnatal developmental processes. genesis 49:419-422, 2011. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/21462293/Generation_of_Osr1_conditional_mutant_mice_ L2 - https://doi.org/10.1002/dvg.20734 DB - PRIME DP - Unbound Medicine ER -