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Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena).
Environ Sci Pollut Res Int. 2011 Sep; 18(8):1360-73.ES

Abstract

PURPOSE

The dyes and dye stuffs present in effluents released from textile dyeing industries are potentially mutagenic and carcinogenic. Phytoremediation technology can be used for remediating sites contaminated with such textile dyeing effluents. The purpose of the work was to explore the potential of Glandularia pulchella (Sweet) Tronc. to decolorize different textile dyes, textile dyeing effluent, and synthetic mixture of dyes.

METHODS

Enzymatic analysis of the plant roots was performed before and after decolorization of dye Green HE4B. Analysis of the metabolites of Green HE4B degradation was done using UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), and gas chromatography-mass spectroscopy (GC-MS). The ability of the plant to decolorize and detoxify a textile dyeing effluent and a synthetic mixture of dyes was studied by a determination of the American Dye Manufacturer's Institute (ADMI), biological oxygen demand (BOD), and chemical oxygen demand (COD). Phytotoxicity studies were performed.

RESULT

Induction of the activities of lignin peroxidase, laccase, tyrosinase, and 2,6-dichlorophenol indophenol reductase was obtained, suggesting their involvement in the dye degradation. UV-Vis spectroscopy, HPLC, and FTIR analysis confirmed the degradation of the dye. Three metabolites of the dye degradation were identified, namely, 1-(4-methylphenyl)-2-{7-[(Z)-phenyldiazenyl] naphthalen-2-yl} diazene; 7,8-diamino-2-(phenyldiazenyl) naphthalen-1-ol; and (Z)-1,1'-naphthalene-2,7-diylbis (phenyldiazene) using GC-MS. ADMI, BOD, and COD values were reduced. The non-toxic nature of the metabolites of Green HE4B degradation was revealed by phytotoxicity studies.

CONCLUSION

This study explored the phytoremediation ability of G. pulchella (Sweet) Tronc. in degrading Green HE4B into non-toxic metabolites.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Kabra AN
Department of Biotechnology, Shivaji University, Kolhapur, India.
Khandare RV
No affiliation info available
Kurade MB
No affiliation info available
Govindwar SP
No affiliation info available

MeSH

Azo CompoundsBiodegradation, EnvironmentalBiological Oxygen Demand AnalysisChromatography, High Pressure LiquidGas Chromatography-Mass SpectrometryGerminationPlant RootsSeedsSpectroscopy, Fourier Transform InfraredTextile IndustryVerbena

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21465161

Citation

Kabra, Akhil N., et al. "Phytoremediation of a Sulphonated Azo Dye Green HE4B By Glandularia Pulchella (Sweet) Tronc. (Moss Verbena)." Environmental Science and Pollution Research International, vol. 18, no. 8, 2011, pp. 1360-73.
Kabra AN, Khandare RV, Kurade MB, et al. Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena). Environ Sci Pollut Res Int. 2011;18(8):1360-73.
Kabra, A. N., Khandare, R. V., Kurade, M. B., & Govindwar, S. P. (2011). Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena). Environmental Science and Pollution Research International, 18(8), 1360-73. https://doi.org/10.1007/s11356-011-0491-7
Kabra AN, et al. Phytoremediation of a Sulphonated Azo Dye Green HE4B By Glandularia Pulchella (Sweet) Tronc. (Moss Verbena). Environ Sci Pollut Res Int. 2011;18(8):1360-73. PubMed PMID: 21465161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytoremediation of a sulphonated azo dye Green HE4B by Glandularia pulchella (Sweet) Tronc. (Moss Verbena). AU - Kabra,Akhil N, AU - Khandare,Rahul V, AU - Kurade,Mayur B, AU - Govindwar,Sanjay P, Y1 - 2011/04/05/ PY - 2010/06/11/received PY - 2011/03/11/accepted PY - 2011/4/6/entrez PY - 2011/4/6/pubmed PY - 2011/10/15/medline SP - 1360 EP - 73 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 18 IS - 8 N2 - PURPOSE: The dyes and dye stuffs present in effluents released from textile dyeing industries are potentially mutagenic and carcinogenic. Phytoremediation technology can be used for remediating sites contaminated with such textile dyeing effluents. The purpose of the work was to explore the potential of Glandularia pulchella (Sweet) Tronc. to decolorize different textile dyes, textile dyeing effluent, and synthetic mixture of dyes. METHODS: Enzymatic analysis of the plant roots was performed before and after decolorization of dye Green HE4B. Analysis of the metabolites of Green HE4B degradation was done using UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), and gas chromatography-mass spectroscopy (GC-MS). The ability of the plant to decolorize and detoxify a textile dyeing effluent and a synthetic mixture of dyes was studied by a determination of the American Dye Manufacturer's Institute (ADMI), biological oxygen demand (BOD), and chemical oxygen demand (COD). Phytotoxicity studies were performed. RESULT: Induction of the activities of lignin peroxidase, laccase, tyrosinase, and 2,6-dichlorophenol indophenol reductase was obtained, suggesting their involvement in the dye degradation. UV-Vis spectroscopy, HPLC, and FTIR analysis confirmed the degradation of the dye. Three metabolites of the dye degradation were identified, namely, 1-(4-methylphenyl)-2-{7-[(Z)-phenyldiazenyl] naphthalen-2-yl} diazene; 7,8-diamino-2-(phenyldiazenyl) naphthalen-1-ol; and (Z)-1,1'-naphthalene-2,7-diylbis (phenyldiazene) using GC-MS. ADMI, BOD, and COD values were reduced. The non-toxic nature of the metabolites of Green HE4B degradation was revealed by phytotoxicity studies. CONCLUSION: This study explored the phytoremediation ability of G. pulchella (Sweet) Tronc. in degrading Green HE4B into non-toxic metabolites. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/21465161/Phytoremediation_of_a_sulphonated_azo_dye_Green_HE4B_by_Glandularia_pulchella__Sweet__Tronc___Moss_Verbena__ L2 - https://dx.doi.org/10.1007/s11356-011-0491-7 DB - PRIME DP - Unbound Medicine ER -