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A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages.
Inflammation. 2012 Feb; 35(1):321-31.I

Abstract

The biological activity of Wercklea insignis (WI) in inflammation and the underlying mechanisms of action of extracts of this plant are largely unknown. In the present study, we investigated the effects of a WI methanolic extract on lipopolysaccharide-stimulated inflammation in the mouse macrophage cell line, RAW 264.7. A WI methanolic extract significantly inhibited NO, PGE(2), IL-6, IL-1β, and TNF-α production in LPS-stimulated RAW 264.7 cells. Expression of iNOS, COX-2, IL-6, IL-1β, and TNF-α were suppressed by the extract at both the mRNA and protein levels in lipopolysaccharide (LPS)-stimulated cells. Additionally, the attenuation of inflammatory responses in RAW 264.7 cells by the WI extract was closely associated with suppression of phosphorylation of mitogen-activated protein kinase (MAPK) molecules, including ERK, JNK1/2, and p38 MAPK and translocation of the nuclear factor (NF)-κB p65 subunit into the nucleus. The effect of WI extract was investigated against carrageenan-induced paw edema in female (20-25 g). Our results collectively indicate that the WI extract inhibits LPS-induced inflammatory responses by blocking the NF-κB signaling pathway in macrophages, supporting use of the extract as a therapeutic anti-inflammatory treatment.

Authors+Show Affiliations

Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-4 Yangchung-ri, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21465277

Citation

Park, Ji-Won, et al. "A Leaf Methanolic Extract of Wercklea Insignis Attenuates the Lipopolysaccharide-induced Inflammatory Response By Blocking the NF-κB Signaling Pathway in RAW 264.7 Macrophages." Inflammation, vol. 35, no. 1, 2012, pp. 321-31.
Park JW, Kwon OK, Jang HY, et al. A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages. Inflammation. 2012;35(1):321-31.
Park, J. W., Kwon, O. K., Jang, H. Y., Jeong, H., Oh, S. R., Lee, H. K., Han, S. B., & Ahn, K. S. (2012). A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages. Inflammation, 35(1), 321-31. https://doi.org/10.1007/s10753-011-9322-8
Park JW, et al. A Leaf Methanolic Extract of Wercklea Insignis Attenuates the Lipopolysaccharide-induced Inflammatory Response By Blocking the NF-κB Signaling Pathway in RAW 264.7 Macrophages. Inflammation. 2012;35(1):321-31. PubMed PMID: 21465277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages. AU - Park,Ji-Won, AU - Kwon,Ok-Kyoung, AU - Jang,Ha-young, AU - Jeong,Hyeok, AU - Oh,Sei-Ryang, AU - Lee,Hyeong-Kyu, AU - Han,Sang-Bae, AU - Ahn,Kyung-Seop, PY - 2011/4/6/entrez PY - 2011/4/6/pubmed PY - 2012/8/1/medline SP - 321 EP - 31 JF - Inflammation JO - Inflammation VL - 35 IS - 1 N2 - The biological activity of Wercklea insignis (WI) in inflammation and the underlying mechanisms of action of extracts of this plant are largely unknown. In the present study, we investigated the effects of a WI methanolic extract on lipopolysaccharide-stimulated inflammation in the mouse macrophage cell line, RAW 264.7. A WI methanolic extract significantly inhibited NO, PGE(2), IL-6, IL-1β, and TNF-α production in LPS-stimulated RAW 264.7 cells. Expression of iNOS, COX-2, IL-6, IL-1β, and TNF-α were suppressed by the extract at both the mRNA and protein levels in lipopolysaccharide (LPS)-stimulated cells. Additionally, the attenuation of inflammatory responses in RAW 264.7 cells by the WI extract was closely associated with suppression of phosphorylation of mitogen-activated protein kinase (MAPK) molecules, including ERK, JNK1/2, and p38 MAPK and translocation of the nuclear factor (NF)-κB p65 subunit into the nucleus. The effect of WI extract was investigated against carrageenan-induced paw edema in female (20-25 g). Our results collectively indicate that the WI extract inhibits LPS-induced inflammatory responses by blocking the NF-κB signaling pathway in macrophages, supporting use of the extract as a therapeutic anti-inflammatory treatment. SN - 1573-2576 UR - https://www.unboundmedicine.com/medline/citation/21465277/A_leaf_methanolic_extract_of_Wercklea_insignis_attenuates_the_lipopolysaccharide_induced_inflammatory_response_by_blocking_the_NF_κB_signaling_pathway_in_RAW_264_7_macrophages_ L2 - https://doi.org/10.1007/s10753-011-9322-8 DB - PRIME DP - Unbound Medicine ER -