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One-step separation of three flavonoids from Poacynum hendersonii by high-speed counter-current chromatography.
Phytochem Anal. 2011 Sep-Oct; 22(5):450-4.PA

Abstract

INTRODUCTION

Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii.

OBJECTIVE

To develop an efficient method for the preparative isolation and purification of flavonoids from the leaves of Poacynum hendersonii by high-speed counter-current chromatography (HSCCC).

METHODOLOGY

Powdered Poacynum hendersonii lead was extracted three times with 75% ethanol at 60 °C for 3 h. The distribution constant (K(D)) was measured to select an optimal two-phase solvent system for HSCCC separation. The purities of the target compounds were tested using HPLC and their structures were identified by ¹H-NMR and ¹³C-NMR.

RESULTS

Using a two-phase solvent system composed of n-butanol-petroleum ether-0.5% acetic acid (5:3:5, v/v), three main flavonoids, i.e. isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6''-O-malonyl)-β-D-glucoside, were separated from 240 mg crude sample in a one-step separation by using HSCCC method. After further purification with a Sepdex-LH20 column, 5.7 mg isoquercitrin (LC purity 98.72%), 4.9 mg quercetin-3-O-sophoroside (LC purity 99.06%) and 7.4 mg quercetin-3-O-(6''-O-malonyl)-β-D-glucoside (LC purity 99.31%) were obtained, respectively.

CONCLUSION

The optimised high-speed counter-current chromatography method is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii.

Authors+Show Affiliations

Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China; Graduate School of the Chinese Academy of Sciences, Beijing 100049, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21465596

Citation

Shi, Junyou, et al. "One-step Separation of Three Flavonoids From Poacynum Hendersonii By High-speed Counter-current Chromatography." Phytochemical Analysis : PCA, vol. 22, no. 5, 2011, pp. 450-4.
Shi J, Li G, Wang H, et al. One-step separation of three flavonoids from Poacynum hendersonii by high-speed counter-current chromatography. Phytochem Anal. 2011;22(5):450-4.
Shi, J., Li, G., Wang, H., Zheng, J., Suo, Y., You, J., & Liu, Y. (2011). One-step separation of three flavonoids from Poacynum hendersonii by high-speed counter-current chromatography. Phytochemical Analysis : PCA, 22(5), 450-4. https://doi.org/10.1002/pca.1301
Shi J, et al. One-step Separation of Three Flavonoids From Poacynum Hendersonii By High-speed Counter-current Chromatography. Phytochem Anal. 2011 Sep-Oct;22(5):450-4. PubMed PMID: 21465596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One-step separation of three flavonoids from Poacynum hendersonii by high-speed counter-current chromatography. AU - Shi,Junyou, AU - Li,Guoliang, AU - Wang,Honglun, AU - Zheng,Jie, AU - Suo,Yourui, AU - You,Jinmao, AU - Liu,Yongjun, Y1 - 2011/04/04/ PY - 2010/07/01/received PY - 2010/10/09/revised PY - 2010/10/14/accepted PY - 2011/4/6/entrez PY - 2011/4/6/pubmed PY - 2012/3/2/medline SP - 450 EP - 4 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 22 IS - 5 N2 - INTRODUCTION: Owing to them having the same traditional name, the leaves of Apoacynum venetum and Poacynum hendersonii are used indiscriminately in some areas of China. Although a series of studies have been conducted on Apoacynum venetum, there are only a few studies on Poacynum hendersonii. OBJECTIVE: To develop an efficient method for the preparative isolation and purification of flavonoids from the leaves of Poacynum hendersonii by high-speed counter-current chromatography (HSCCC). METHODOLOGY: Powdered Poacynum hendersonii lead was extracted three times with 75% ethanol at 60 °C for 3 h. The distribution constant (K(D)) was measured to select an optimal two-phase solvent system for HSCCC separation. The purities of the target compounds were tested using HPLC and their structures were identified by ¹H-NMR and ¹³C-NMR. RESULTS: Using a two-phase solvent system composed of n-butanol-petroleum ether-0.5% acetic acid (5:3:5, v/v), three main flavonoids, i.e. isoquercitrin, quercetin-3-O-sophoroside and quercetin-3-O-(6''-O-malonyl)-β-D-glucoside, were separated from 240 mg crude sample in a one-step separation by using HSCCC method. After further purification with a Sepdex-LH20 column, 5.7 mg isoquercitrin (LC purity 98.72%), 4.9 mg quercetin-3-O-sophoroside (LC purity 99.06%) and 7.4 mg quercetin-3-O-(6''-O-malonyl)-β-D-glucoside (LC purity 99.31%) were obtained, respectively. CONCLUSION: The optimised high-speed counter-current chromatography method is fast, simple and efficient for the preparative separation of flavonoids from the leave of Poacynum hendersonii. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/21465596/One_step_separation_of_three_flavonoids_from_Poacynum_hendersonii_by_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/pca.1301 DB - PRIME DP - Unbound Medicine ER -