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Role of cannabinoid receptors and RAGE in inflammatory bowel disease.

Abstract

BACKGROUND

The endocannabinoid system is involved in many inflammatory diseases, such as Crohn's disease (CD) and ulcerative colitis (UC). The distribution and expression of cannabinoid receptors 1 (CNR1) and 2 (CNR2) in combination with inflammatory cytokines and RAGE (receptor of advanced glycation end products), which is also overactive in these diseases, in dependency of the extent of inflammation and alteration of the colon barrier is still unclear and needs to be elucidated.

MATERIAL AND METHODS

10 specimens of CD patients who underwent colectomy and 14 colectomy specimens of patients suffering from UC were investigated histologically for inflammatory infiltrate, extent of fibrosis and for disturbance of the intestinal barrier. Immunohistochemistry was carried out to examine the distribution and localization of CNR1, CNR2 and RAGE. Additionally, qRT-PCR was performed to study the expression of CNR1, CNR2, RAGE and inflammatory cytokines (TNFα, TGFβ, CTGF, IL12, IFNγ). 35 morphological and histological normal specimens of colectomy cases served as controls.

RESULTS

The expression level of CNR2 did not differ between the control group and the group of patients with IBD, while CNR1 displayed a significant up regulation, especially in cases of CD. A differential association between the expression of CNR1/CNR2 and RAGE with morphological changes and expression of molecular markers of inflammation could be established.

CONCLUSION

We showed that cannabinoid receptors are expressed differentially in inflammatory bowel disease and that the expression seems to be influenced by the underlying disease and by localized inflammation.

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  • Authors+Show Affiliations

    ,

    Department of Medicine III, University Hospital Grosshadern, Marchioninistrasse 15, Munich, Germany. sebastian.stintzing@med.uni-muenchen.de

    , , , ,

    Source

    Histology and histopathology 26:6 2011 06 pg 735-45

    MeSH

    Adolescent
    Adult
    Aged
    Aged, 80 and over
    Blotting, Western
    Female
    Gene Expression
    Gene Expression Profiling
    Humans
    Immunohistochemistry
    Inflammatory Bowel Diseases
    Male
    Middle Aged
    Receptor for Advanced Glycation End Products
    Receptor, Cannabinoid, CB1
    Receptor, Cannabinoid, CB2
    Receptors, Immunologic
    Reverse Transcriptase Polymerase Chain Reaction
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21472688

    Citation

    * When formatting your citation, note that all book, journal, and database titles should be italicized* Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Role of cannabinoid receptors and RAGE in inflammatory bowel disease. AU - Stintzing,Sebastian, AU - Wissniowski,Till Th, AU - Lohwasser,Christina, AU - Alinger,Beate, AU - Neureiter,Daniel, AU - Ocker,Matthias, PY - 2011/4/8/entrez PY - 2011/4/8/pubmed PY - 2011/7/29/medline SP - 735 EP - 45 JF - Histology and histopathology JO - Histol. Histopathol. VL - 26 IS - 6 N2 - BACKGROUND: The endocannabinoid system is involved in many inflammatory diseases, such as Crohn's disease (CD) and ulcerative colitis (UC). The distribution and expression of cannabinoid receptors 1 (CNR1) and 2 (CNR2) in combination with inflammatory cytokines and RAGE (receptor of advanced glycation end products), which is also overactive in these diseases, in dependency of the extent of inflammation and alteration of the colon barrier is still unclear and needs to be elucidated. MATERIAL AND METHODS: 10 specimens of CD patients who underwent colectomy and 14 colectomy specimens of patients suffering from UC were investigated histologically for inflammatory infiltrate, extent of fibrosis and for disturbance of the intestinal barrier. Immunohistochemistry was carried out to examine the distribution and localization of CNR1, CNR2 and RAGE. Additionally, qRT-PCR was performed to study the expression of CNR1, CNR2, RAGE and inflammatory cytokines (TNFα, TGFβ, CTGF, IL12, IFNγ). 35 morphological and histological normal specimens of colectomy cases served as controls. RESULTS: The expression level of CNR2 did not differ between the control group and the group of patients with IBD, while CNR1 displayed a significant up regulation, especially in cases of CD. A differential association between the expression of CNR1/CNR2 and RAGE with morphological changes and expression of molecular markers of inflammation could be established. CONCLUSION: We showed that cannabinoid receptors are expressed differentially in inflammatory bowel disease and that the expression seems to be influenced by the underlying disease and by localized inflammation. SN - 1699-5848 UR - https://www.unboundmedicine.com/medline/citation/21472688/abstract/Role_of_cannabinoid_receptors_and_RAGE_in_inflammatory_bowel_disease_ L2 - http://www.hh.um.es/Abstracts/Vol_26/26_6/26_6_735.htm DB - PRIME DP - Unbound Medicine ER -