Tags

Type your tag names separated by a space and hit enter

Nerve growth factor modulates TRPV1 expression and function and mediates pain in chronic pancreatitis.
Gastroenterology. 2011 Jul; 141(1):370-7.G

Abstract

BACKGROUND & AIMS

The pathogenesis of pain in chronic pancreatitis (CP) is poorly understood and treatment remains difficult. We hypothesized that nerve growth factor (NGF) plays a key role in this process via its effects on the transient receptor potential vanilloid 1, TRPV1.

METHODS

CP was induced by intraductal injection of trinitrobenzene sulfonic acid in rats. After 3 weeks, anti-NGF antibody or control serum was administered daily for 1 week. Pancreatic hyperalgesia was assessed by nocifensive behavioral response to electrical stimulation of the pancreas as well as by referred somatic pain assessed by von Frey filament testing. TRPV1 currents in pancreatic sensory neurons were examined by patch-clamp. The expression and function of TRPV1 in pancreas-specific nociceptors was examined by immunostaining and quantification of messenger RNA levels.

RESULTS

Blockade of NGF significantly attenuated pancreatic hyperalgesia and referred somatic pain compared with controls. It also decreased TRPV1 current density and open probability and reduced the proportion of pancreatic sensory neurons that expressed TRPV1 as well as levels of TRPV1 in these neurons.

CONCLUSIONS

These findings emphasize a key role for NGF in pancreatic pain and highlight the role it plays in the modulation of TRPV1 expression and activity in CP.

Authors+Show Affiliations

Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21473865

Citation

Zhu, Yaohui, et al. "Nerve Growth Factor Modulates TRPV1 Expression and Function and Mediates Pain in Chronic Pancreatitis." Gastroenterology, vol. 141, no. 1, 2011, pp. 370-7.
Zhu Y, Colak T, Shenoy M, et al. Nerve growth factor modulates TRPV1 expression and function and mediates pain in chronic pancreatitis. Gastroenterology. 2011;141(1):370-7.
Zhu, Y., Colak, T., Shenoy, M., Liu, L., Pai, R., Li, C., Mehta, K., & Pasricha, P. J. (2011). Nerve growth factor modulates TRPV1 expression and function and mediates pain in chronic pancreatitis. Gastroenterology, 141(1), 370-7. https://doi.org/10.1053/j.gastro.2011.03.046
Zhu Y, et al. Nerve Growth Factor Modulates TRPV1 Expression and Function and Mediates Pain in Chronic Pancreatitis. Gastroenterology. 2011;141(1):370-7. PubMed PMID: 21473865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nerve growth factor modulates TRPV1 expression and function and mediates pain in chronic pancreatitis. AU - Zhu,Yaohui, AU - Colak,Tugba, AU - Shenoy,Mohan, AU - Liu,Liansheng, AU - Pai,Reetesh, AU - Li,Cuiping, AU - Mehta,Kshama, AU - Pasricha,Pankaj Jay, Y1 - 2011/04/05/ PY - 2010/12/23/received PY - 2011/02/09/revised PY - 2011/03/07/accepted PY - 2011/4/9/entrez PY - 2011/4/9/pubmed PY - 2011/9/8/medline SP - 370 EP - 7 JF - Gastroenterology JO - Gastroenterology VL - 141 IS - 1 N2 - BACKGROUND & AIMS: The pathogenesis of pain in chronic pancreatitis (CP) is poorly understood and treatment remains difficult. We hypothesized that nerve growth factor (NGF) plays a key role in this process via its effects on the transient receptor potential vanilloid 1, TRPV1. METHODS: CP was induced by intraductal injection of trinitrobenzene sulfonic acid in rats. After 3 weeks, anti-NGF antibody or control serum was administered daily for 1 week. Pancreatic hyperalgesia was assessed by nocifensive behavioral response to electrical stimulation of the pancreas as well as by referred somatic pain assessed by von Frey filament testing. TRPV1 currents in pancreatic sensory neurons were examined by patch-clamp. The expression and function of TRPV1 in pancreas-specific nociceptors was examined by immunostaining and quantification of messenger RNA levels. RESULTS: Blockade of NGF significantly attenuated pancreatic hyperalgesia and referred somatic pain compared with controls. It also decreased TRPV1 current density and open probability and reduced the proportion of pancreatic sensory neurons that expressed TRPV1 as well as levels of TRPV1 in these neurons. CONCLUSIONS: These findings emphasize a key role for NGF in pancreatic pain and highlight the role it plays in the modulation of TRPV1 expression and activity in CP. SN - 1528-0012 UR - https://www.unboundmedicine.com/medline/citation/21473865/Nerve_growth_factor_modulates_TRPV1_expression_and_function_and_mediates_pain_in_chronic_pancreatitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5085(11)00417-3 DB - PRIME DP - Unbound Medicine ER -