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In vitro antioxidant and radical scavenging of Guinean kinkeliba leaf (Combretum micranthum G. Don) extracts.
Nat Prod Res. 2011 Jul; 25(11):1025-36.NP

Abstract

The antioxidant properties of the water and ethanol leaf extracts of kinkeliba (Combretum micranthum) were investigated, including scavenging of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (IC₅₀ values: 8.02 ± 0.34 for the ethanol extract [KE] and 9.1 ± 0.28 for the water extract [KW]), the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical (IC(50) values: 7.4 ± 0.14 for KE and 11.8 ± 0.01 for KW) and the hydroxyl radical (58.1% for KE and 61.1% for KW). The ferric thiocyanate method, reducing power, metal chelating activity, an assay of protein oxidation and the β-carotene-linoleic bleaching assay were also used. Butylated hydroxytoluene and ascorbic acid were used as the reference antioxidant compounds. At 20 mg mL⁻¹ concentration, KW and KE provided 36.8% and 75.1% inhibition of lipid peroxidation of linoleic acid emulsion, respectively. The IC₅₀ values of the ethanol extract in ABTS and DPPH tests were significantly lower than those from the water extract. Furthermore, crude polyphenols were extracted from kinkeliba leaf with 90% ethanol solution using a water bath treatment and then purified by a macroporous resin, AB-8. The polyphenols from kinkeliba leaf were subjected to analyses by RP-HPLC and ESI-MS. The dominant polyphenols in kinkeliba leaf were identified as gallic acid, rutin trihydrate, (+)-catechin and benzoic acid.

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Authors+Show Affiliations

Touré A
State Key Laboratory of the School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, P.R. China.
Xu X
No affiliation info available
Michel T
No affiliation info available
Bangoura M
No affiliation info available

MeSH

AntioxidantsBenzoic AcidCatechinChromatography, High Pressure LiquidCombretumFree Radical ScavengersLipid PeroxidationMass SpectrometryPlant ExtractsPlant LeavesPolyphenolsRutin

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21480049

Citation

Touré, Alhassane, et al. "In Vitro Antioxidant and Radical Scavenging of Guinean Kinkeliba Leaf (Combretum Micranthum G. Don) Extracts." Natural Product Research, vol. 25, no. 11, 2011, pp. 1025-36.
Touré A, Xu X, Michel T, et al. In vitro antioxidant and radical scavenging of Guinean kinkeliba leaf (Combretum micranthum G. Don) extracts. Nat Prod Res. 2011;25(11):1025-36.
Touré, A., Xu, X., Michel, T., & Bangoura, M. (2011). In vitro antioxidant and radical scavenging of Guinean kinkeliba leaf (Combretum micranthum G. Don) extracts. Natural Product Research, 25(11), 1025-36. https://doi.org/10.1080/14786419.2010.482048
Touré A, et al. In Vitro Antioxidant and Radical Scavenging of Guinean Kinkeliba Leaf (Combretum Micranthum G. Don) Extracts. Nat Prod Res. 2011;25(11):1025-36. PubMed PMID: 21480049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant and radical scavenging of Guinean kinkeliba leaf (Combretum micranthum G. Don) extracts. AU - Touré,Alhassane, AU - Xu,Xueming, AU - Michel,T, AU - Bangoura,M, PY - 2011/4/12/entrez PY - 2011/4/12/pubmed PY - 2011/12/13/medline SP - 1025 EP - 36 JF - Natural product research JO - Nat Prod Res VL - 25 IS - 11 N2 - The antioxidant properties of the water and ethanol leaf extracts of kinkeliba (Combretum micranthum) were investigated, including scavenging of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (IC₅₀ values: 8.02 ± 0.34 for the ethanol extract [KE] and 9.1 ± 0.28 for the water extract [KW]), the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical (IC(50) values: 7.4 ± 0.14 for KE and 11.8 ± 0.01 for KW) and the hydroxyl radical (58.1% for KE and 61.1% for KW). The ferric thiocyanate method, reducing power, metal chelating activity, an assay of protein oxidation and the β-carotene-linoleic bleaching assay were also used. Butylated hydroxytoluene and ascorbic acid were used as the reference antioxidant compounds. At 20 mg mL⁻¹ concentration, KW and KE provided 36.8% and 75.1% inhibition of lipid peroxidation of linoleic acid emulsion, respectively. The IC₅₀ values of the ethanol extract in ABTS and DPPH tests were significantly lower than those from the water extract. Furthermore, crude polyphenols were extracted from kinkeliba leaf with 90% ethanol solution using a water bath treatment and then purified by a macroporous resin, AB-8. The polyphenols from kinkeliba leaf were subjected to analyses by RP-HPLC and ESI-MS. The dominant polyphenols in kinkeliba leaf were identified as gallic acid, rutin trihydrate, (+)-catechin and benzoic acid. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/21480049/In_vitro_antioxidant_and_radical_scavenging_of_Guinean_kinkeliba_leaf__Combretum_micranthum_G__Don__extracts_ L2 - https://www.tandfonline.com/doi/full/10.1080/14786419.2010.482048 DB - PRIME DP - Unbound Medicine ER -