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Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria.
Folia Microbiol (Praha) 1990; 35(4):273-7FM

Abstract

A new shuttle vector pCEM500 replicating in Escherichia coli and in Brevibacterium flavum was constructed. It carries two antibiotic resistance determinants (Kmr/Gmr from plasmid pSa of Gram-negative bacteria and Smr/Spr from plasmid pCG4 of Corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of DNA fragments into the unique restriction endonuclease sites located within them. This vector was found to be stably maintained in B. flavum and can be used for transfer of the cloned genes into this amino-acid-producing coryneform bacterium.

Authors+Show Affiliations

Department of Microbial Genetics, Czechoslovak Academy of Sciences, Prague.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2148164

Citation

Nesvera, J, et al. "Plasmid Shuttle Vector With Two Insertionally Inactivable Markers for Coryneform Bacteria." Folia Microbiologica, vol. 35, no. 4, 1990, pp. 273-7.
Nesvera J, Pátek M, Hochmannová J, et al. Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. Folia Microbiol (Praha). 1990;35(4):273-7.
Nesvera, J., Pátek, M., Hochmannová, J., & Pinkas, P. (1990). Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. Folia Microbiologica, 35(4), pp. 273-7.
Nesvera J, et al. Plasmid Shuttle Vector With Two Insertionally Inactivable Markers for Coryneform Bacteria. Folia Microbiol (Praha). 1990;35(4):273-7. PubMed PMID: 2148164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. AU - Nesvera,J, AU - Pátek,M, AU - Hochmannová,J, AU - Pinkas,P, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 273 EP - 7 JF - Folia microbiologica JO - Folia Microbiol. (Praha) VL - 35 IS - 4 N2 - A new shuttle vector pCEM500 replicating in Escherichia coli and in Brevibacterium flavum was constructed. It carries two antibiotic resistance determinants (Kmr/Gmr from plasmid pSa of Gram-negative bacteria and Smr/Spr from plasmid pCG4 of Corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of DNA fragments into the unique restriction endonuclease sites located within them. This vector was found to be stably maintained in B. flavum and can be used for transfer of the cloned genes into this amino-acid-producing coryneform bacterium. SN - 0015-5632 UR - https://www.unboundmedicine.com/medline/citation/2148164/Plasmid_shuttle_vector_with_two_insertionally_inactivable_markers_for_coryneform_bacteria_ DB - PRIME DP - Unbound Medicine ER -