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Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence.
Vet Microbiol. 2011 Aug 05; 151(3-4):400-3.VM

Abstract

Corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. Although some genes have been studied for diagnostic and phylogenetic analysis within the genus Corynebacterium, at subspecies level the pathogen has been poorly analyzed. The aim of this study was to characterize C. pseudotuberculosis strains isolated from domestic animals, through the sequencing of a hypervariable rpoB gene segment. As result, there were identified host associated rpoB polymorphisms in strains infecting sheep, goats and horses from Chile. These differences suggest the existence of bacterial genotypes, in which the nucleotide similarity values were ranging from 98.8 to 99.8%. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can be used as a diagnostic tool that differentiates C. pseudotuberculosis strains at subspecies level.

Authors+Show Affiliations

Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile. Casilla 2, Correo 15, Santiago, Chile. pretamal@uchile.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21482046

Citation

Retamal, Patricio, et al. "Host Associated Polymorphisms in the Corynebacterium Pseudotuberculosis rpoB Gene Sequence." Veterinary Microbiology, vol. 151, no. 3-4, 2011, pp. 400-3.
Retamal P, Ríos M, Cheuquepán F, et al. Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence. Vet Microbiol. 2011;151(3-4):400-3.
Retamal, P., Ríos, M., Cheuquepán, F., Abalos, P., Pizarro-Lucero, J., Borie, C., & Gutierrez, J. (2011). Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence. Veterinary Microbiology, 151(3-4), 400-3. https://doi.org/10.1016/j.vetmic.2011.03.012
Retamal P, et al. Host Associated Polymorphisms in the Corynebacterium Pseudotuberculosis rpoB Gene Sequence. Vet Microbiol. 2011 Aug 5;151(3-4):400-3. PubMed PMID: 21482046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Host associated polymorphisms in the Corynebacterium pseudotuberculosis rpoB gene sequence. AU - Retamal,Patricio, AU - Ríos,Marco, AU - Cheuquepán,Felipe, AU - Abalos,Pedro, AU - Pizarro-Lucero,José, AU - Borie,Consuelo, AU - Gutierrez,Josefina, Y1 - 2011/03/21/ PY - 2010/10/22/received PY - 2011/03/08/revised PY - 2011/03/14/accepted PY - 2011/4/13/entrez PY - 2011/4/13/pubmed PY - 2011/10/19/medline SP - 400 EP - 3 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 151 IS - 3-4 N2 - Corynebacterium pseudotuberculosis is a widespread facultative intracellular pathogen that causes caseous lymphadenitis disease in sheep and goats, and generates cutaneous abscesses and granulomas in horses and cattle. Although some genes have been studied for diagnostic and phylogenetic analysis within the genus Corynebacterium, at subspecies level the pathogen has been poorly analyzed. The aim of this study was to characterize C. pseudotuberculosis strains isolated from domestic animals, through the sequencing of a hypervariable rpoB gene segment. As result, there were identified host associated rpoB polymorphisms in strains infecting sheep, goats and horses from Chile. These differences suggest the existence of bacterial genotypes, in which the nucleotide similarity values were ranging from 98.8 to 99.8%. In conclusion, the analysis of polymorphisms in the partial rpoB sequence can be used as a diagnostic tool that differentiates C. pseudotuberculosis strains at subspecies level. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/21482046/Host_associated_polymorphisms_in_the_Corynebacterium_pseudotuberculosis_rpoB_gene_sequence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(11)00150-7 DB - PRIME DP - Unbound Medicine ER -