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Molecularly imprinted polymer for selective extraction of domoic acid from seafood coupled with high-performance liquid chromatographic determination.
Talanta. 2011 May 15; 84(3):777-82.T

Abstract

In this work, a highly selective sample cleanup procedure that combining molecular imprinting technique (MIT) and solid phase extraction (SPE) was developed for the isolation of domoic acid (a fascinating marine toxin) from seafood samples. The molecular imprinting polymer (MIP) for domoic acid was prepared using 1,3,5-pentanetricarboxylic acid as the template molecule instead of domoic acid. 4-Vinyl pyridine was used as the functional monomer and ethylene glycol dimethacrylate was used as the cross-linking monomer. The obtained imprinted polymer showed high affinity to domoic acid and was used as selective sorbent for the SPE of domoic acid from seafood samples. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by high-performance liquid chromatography (HPLC) with diode-array detection for the detection of domoic acid was also established. Good linearity was obtained from 0.5 mg L(-1) to 25 mg L(-1) (R(2)>0.99) with a quantitation limit of 0.1 mg L(-1), which was sufficient to determine domoic acid at the maximum level permitted by several authorities. The mean recoveries of domoic acid from mussel extracts were 93.4-96.7%. It was demonstrated that the proposed MISPE-HPLC method could be applied to direct determination of domoic acid from seafood samples.

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Authors+Show Affiliations

Zhou WH
The Key Laboratory for Special Functional Materials of MOE, Henan University, Kaifeng, PR China. zhouwh@henu.edu.cn
Guo XC
No affiliation info available
Zhao HQ
No affiliation info available
Wu SX
No affiliation info available
Yang HH
No affiliation info available
Wang XR
No affiliation info available

MeSH

AnimalsBivalviaChromatography, High Pressure LiquidKainic AcidLimit of DetectionPolymersReproducibility of ResultsSeafoodSolid Phase Extraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21482282

Citation

Zhou, Wen-Hui, et al. "Molecularly Imprinted Polymer for Selective Extraction of Domoic Acid From Seafood Coupled With High-performance Liquid Chromatographic Determination." Talanta, vol. 84, no. 3, 2011, pp. 777-82.
Zhou WH, Guo XC, Zhao HQ, et al. Molecularly imprinted polymer for selective extraction of domoic acid from seafood coupled with high-performance liquid chromatographic determination. Talanta. 2011;84(3):777-82.
Zhou, W. H., Guo, X. C., Zhao, H. Q., Wu, S. X., Yang, H. H., & Wang, X. R. (2011). Molecularly imprinted polymer for selective extraction of domoic acid from seafood coupled with high-performance liquid chromatographic determination. Talanta, 84(3), 777-82. https://doi.org/10.1016/j.talanta.2011.02.004
Zhou WH, et al. Molecularly Imprinted Polymer for Selective Extraction of Domoic Acid From Seafood Coupled With High-performance Liquid Chromatographic Determination. Talanta. 2011 May 15;84(3):777-82. PubMed PMID: 21482282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted polymer for selective extraction of domoic acid from seafood coupled with high-performance liquid chromatographic determination. AU - Zhou,Wen-Hui, AU - Guo,Xiu-Chun, AU - Zhao,Heng-Qiang, AU - Wu,Si-Xin, AU - Yang,Huang-Hao, AU - Wang,Xiao-Ru, Y1 - 2011/02/25/ PY - 2010/11/03/received PY - 2011/01/27/revised PY - 2011/02/08/accepted PY - 2011/4/13/entrez PY - 2011/4/13/pubmed PY - 2011/7/23/medline SP - 777 EP - 82 JF - Talanta JO - Talanta VL - 84 IS - 3 N2 - In this work, a highly selective sample cleanup procedure that combining molecular imprinting technique (MIT) and solid phase extraction (SPE) was developed for the isolation of domoic acid (a fascinating marine toxin) from seafood samples. The molecular imprinting polymer (MIP) for domoic acid was prepared using 1,3,5-pentanetricarboxylic acid as the template molecule instead of domoic acid. 4-Vinyl pyridine was used as the functional monomer and ethylene glycol dimethacrylate was used as the cross-linking monomer. The obtained imprinted polymer showed high affinity to domoic acid and was used as selective sorbent for the SPE of domoic acid from seafood samples. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by high-performance liquid chromatography (HPLC) with diode-array detection for the detection of domoic acid was also established. Good linearity was obtained from 0.5 mg L(-1) to 25 mg L(-1) (R(2)>0.99) with a quantitation limit of 0.1 mg L(-1), which was sufficient to determine domoic acid at the maximum level permitted by several authorities. The mean recoveries of domoic acid from mussel extracts were 93.4-96.7%. It was demonstrated that the proposed MISPE-HPLC method could be applied to direct determination of domoic acid from seafood samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/21482282/Molecularly_imprinted_polymer_for_selective_extraction_of_domoic_acid_from_seafood_coupled_with_high_performance_liquid_chromatographic_determination_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00135-4 DB - PRIME DP - Unbound Medicine ER -