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Generation of mice with a novel conditional null allele of the Sox9 gene.
Biotechnol Lett. 2011 Aug; 33(8):1551-8.BL

Abstract

Sox9 is expressed in multiple tissues during mouse development and adulthood. Mutations in the Sox9 gene or changes in expression levels can be attributed to many congenital diseases. Heterozygous loss-of-function mutations in the human SOX9 gene cause Campomelic dysplasia, a semi-lethal skeletal malformation syndrome. Disruption of Sox9 by conventional gene targeting leads to perinatal lethality in heterozygous mice, hence hampering the feasibility to obtain the homozygous Sox9 null mice for in vivo functional studies. In this study, we generated a conditional allele of Sox9 (Sox9 (tm4.Tlu)) by flanking exon 1 with loxP sites. Homozygous mice for the Sox9 (tm4.Tlu) allele (Sox9 (flox/flox)) are viable, fertile and indistinguishable from wildtype (WT) mice, indicating that the Sox9 (tm4.Tlu) allele is a fully functional Sox9 allele. Furthermore, we demonstrated that Cre-mediated recombination using a Col2a1-Cre line resulted in specific ablation of Sox9 activity in cartilage tissues.

Authors+Show Affiliations

Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore. yaps@gis.a-star.edu.sgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21484342

Citation

Yap, Sook Peng, et al. "Generation of Mice With a Novel Conditional Null Allele of the Sox9 Gene." Biotechnology Letters, vol. 33, no. 8, 2011, pp. 1551-8.
Yap SP, Xing X, Kraus P, et al. Generation of mice with a novel conditional null allele of the Sox9 gene. Biotechnol Lett. 2011;33(8):1551-8.
Yap, S. P., Xing, X., Kraus, P., Sivakamasundari, V., Chan, H. Y., & Lufkin, T. (2011). Generation of mice with a novel conditional null allele of the Sox9 gene. Biotechnology Letters, 33(8), 1551-8. https://doi.org/10.1007/s10529-011-0608-6
Yap SP, et al. Generation of Mice With a Novel Conditional Null Allele of the Sox9 Gene. Biotechnol Lett. 2011;33(8):1551-8. PubMed PMID: 21484342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of mice with a novel conditional null allele of the Sox9 gene. AU - Yap,Sook Peng, AU - Xing,Xing, AU - Kraus,Petra, AU - Sivakamasundari,V, AU - Chan,Hsiao Yun, AU - Lufkin,Thomas, Y1 - 2011/04/12/ PY - 2011/01/14/received PY - 2011/03/29/accepted PY - 2011/4/13/entrez PY - 2011/4/13/pubmed PY - 2011/12/14/medline SP - 1551 EP - 8 JF - Biotechnology letters JO - Biotechnol. Lett. VL - 33 IS - 8 N2 - Sox9 is expressed in multiple tissues during mouse development and adulthood. Mutations in the Sox9 gene or changes in expression levels can be attributed to many congenital diseases. Heterozygous loss-of-function mutations in the human SOX9 gene cause Campomelic dysplasia, a semi-lethal skeletal malformation syndrome. Disruption of Sox9 by conventional gene targeting leads to perinatal lethality in heterozygous mice, hence hampering the feasibility to obtain the homozygous Sox9 null mice for in vivo functional studies. In this study, we generated a conditional allele of Sox9 (Sox9 (tm4.Tlu)) by flanking exon 1 with loxP sites. Homozygous mice for the Sox9 (tm4.Tlu) allele (Sox9 (flox/flox)) are viable, fertile and indistinguishable from wildtype (WT) mice, indicating that the Sox9 (tm4.Tlu) allele is a fully functional Sox9 allele. Furthermore, we demonstrated that Cre-mediated recombination using a Col2a1-Cre line resulted in specific ablation of Sox9 activity in cartilage tissues. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/21484342/Generation_of_mice_with_a_novel_conditional_null_allele_of_the_Sox9_gene_ L2 - https://doi.org/10.1007/s10529-011-0608-6 DB - PRIME DP - Unbound Medicine ER -