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Development of immature thymocytes: initiation of CD3, CD4, and CD8 acquisition parallels down-regulation of the interleukin 2 receptor alpha chain.
Eur J Immunol. 1990 Dec; 20(12):2813-5.EJ

Abstract

We have previously identified a developmental sequence among immature thymocytes, prior to their expression of the lineage markers CD3, CD4, and CD8. This sequence is marked by transient expression of the interleukin 2 receptor alpha chain (IL 2R alpha). The most mature cells in this sequence (surface phenotype heat-stable antigen (HSA)++ Pgp-1- IL 2R alpha-) are the immediate precursors to CD4+CD8+ small cortical thymocytes, and have by definition been considered to be CD4-CD8-. We now show that these cells display low levels of surface CD4 and CD8, but not CD3. This low-level expression begins to appear immediately after the loss of IL 2R alpha expression. Northern blot analysis for mRNA expression confirms that these IL 2R alpha- cells are transcribing CD4 and CD8 mRNA, in contrast to their immediate (IL 2R alpha+) precursor. Upon unstimulated culture, these IL 2R alpha- cells gradually acquire high levels of CD4 and CD8, as well as low levels of CD3, whereas IL 2R alpha+ cells do not. These findings suggest that the IL 2R alpha+ subset is the end of the true CD3-CD4-CD8- phase, and that the intracellular signals for CD3, CD4, and CD8 acquisition occur simultaneously with, or immediately prior to, the signal for down-regulation of IL 2R alpha.

Authors+Show Affiliations

Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2148525

Citation

Petrie, H T., et al. "Development of Immature Thymocytes: Initiation of CD3, CD4, and CD8 Acquisition Parallels Down-regulation of the Interleukin 2 Receptor Alpha Chain." European Journal of Immunology, vol. 20, no. 12, 1990, pp. 2813-5.
Petrie HT, Pearse M, Scollay R, et al. Development of immature thymocytes: initiation of CD3, CD4, and CD8 acquisition parallels down-regulation of the interleukin 2 receptor alpha chain. Eur J Immunol. 1990;20(12):2813-5.
Petrie, H. T., Pearse, M., Scollay, R., & Shortman, K. (1990). Development of immature thymocytes: initiation of CD3, CD4, and CD8 acquisition parallels down-regulation of the interleukin 2 receptor alpha chain. European Journal of Immunology, 20(12), 2813-5.
Petrie HT, et al. Development of Immature Thymocytes: Initiation of CD3, CD4, and CD8 Acquisition Parallels Down-regulation of the Interleukin 2 Receptor Alpha Chain. Eur J Immunol. 1990;20(12):2813-5. PubMed PMID: 2148525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of immature thymocytes: initiation of CD3, CD4, and CD8 acquisition parallels down-regulation of the interleukin 2 receptor alpha chain. AU - Petrie,H T, AU - Pearse,M, AU - Scollay,R, AU - Shortman,K, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 2813 EP - 5 JF - European journal of immunology JO - Eur J Immunol VL - 20 IS - 12 N2 - We have previously identified a developmental sequence among immature thymocytes, prior to their expression of the lineage markers CD3, CD4, and CD8. This sequence is marked by transient expression of the interleukin 2 receptor alpha chain (IL 2R alpha). The most mature cells in this sequence (surface phenotype heat-stable antigen (HSA)++ Pgp-1- IL 2R alpha-) are the immediate precursors to CD4+CD8+ small cortical thymocytes, and have by definition been considered to be CD4-CD8-. We now show that these cells display low levels of surface CD4 and CD8, but not CD3. This low-level expression begins to appear immediately after the loss of IL 2R alpha expression. Northern blot analysis for mRNA expression confirms that these IL 2R alpha- cells are transcribing CD4 and CD8 mRNA, in contrast to their immediate (IL 2R alpha+) precursor. Upon unstimulated culture, these IL 2R alpha- cells gradually acquire high levels of CD4 and CD8, as well as low levels of CD3, whereas IL 2R alpha+ cells do not. These findings suggest that the IL 2R alpha+ subset is the end of the true CD3-CD4-CD8- phase, and that the intracellular signals for CD3, CD4, and CD8 acquisition occur simultaneously with, or immediately prior to, the signal for down-regulation of IL 2R alpha. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/2148525/Development_of_immature_thymocytes:_initiation_of_CD3_CD4_and_CD8_acquisition_parallels_down_regulation_of_the_interleukin_2_receptor_alpha_chain_ L2 - https://doi.org/10.1002/eji.1830201243 DB - PRIME DP - Unbound Medicine ER -