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Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica.
Acta Pol Pharm. 2011 Jan-Feb; 68(1):23-9.AP

Abstract

This study was undertaken to assess in vitro lipid peroxidation inhibitions and anti-radical activities of methanolic, chloroform, ethyl acetate and water fractions of Mangifera indica leaf. Inhibition of Fe(2+)-induced lipid peroxidation (LPO) in egg, brain, and liver homogenates, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH-) radical scavenging activities were evaluated. Total phenol was assessed in all fractions, and the reducing power of methanolic fraction was compared to gallic acid and ascorbic acid. The results showed that Fe2+ induced significant lipid peroxidation (LPO) in all the homogenates. Ethyl acetate fraction showed the highest percentage inhibition of LPO in both egg yolk (68.3%) and brain (66.3%), while the aqueous fraction exerted the highest inhibition in liver homogenate (89.1%) at a concentration of 10 microg/mL. These observed inhibitions of LPO by these fractions were higher than that of ascorbic acid used as a standard. The DPPH radical scavenging ability exhibited by ethyl acetate fraction was found to be the highest with IC50 value of 1.5 microg/mL. The ethyl acetate and methanolic fractions had the highest OH- radical scavenging ability with the same IC50 value of 5 microg/mL. The total phenol content of ethyl acetate fraction was the highest with 0.127 microg/mg gallic acid equivalent (GAE). The reductive potential of methanolic fraction showed a concentration-dependent increase. This study showed that inhibition of LPO and the DPPH and OH- radicals scavenging abilities of Mangifera indica leaf could be related to the presence of phenolic compounds. Therefore, the ethyl acetate fraction of the leaf may be a good source of natural antioxidative agent.

Authors+Show Affiliations

Department of Biochemistry, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria. jabadmus@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

21485698

Citation

Badmus, Jelili A., et al. "Lipid Peroxidation Inhibition and Antiradical Activities of some Leaf Fractions of Mangifera Indica." Acta Poloniae Pharmaceutica, vol. 68, no. 1, 2011, pp. 23-9.
Badmus JA, Adedosu TO, Fatoki JO, et al. Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica. Acta Pol Pharm. 2011;68(1):23-9.
Badmus, J. A., Adedosu, T. O., Fatoki, J. O., Adegbite, V. A., Adaramoye, O. A., & Odunola, O. A. (2011). Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica. Acta Poloniae Pharmaceutica, 68(1), 23-9.
Badmus JA, et al. Lipid Peroxidation Inhibition and Antiradical Activities of some Leaf Fractions of Mangifera Indica. Acta Pol Pharm. 2011 Jan-Feb;68(1):23-9. PubMed PMID: 21485698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica. AU - Badmus,Jelili A, AU - Adedosu,Temitope O, AU - Fatoki,John O, AU - Adegbite,Victor A, AU - Adaramoye,Oluwatosin A, AU - Odunola,Oyeronke A, PY - 2011/4/14/entrez PY - 2011/4/14/pubmed PY - 2011/5/12/medline SP - 23 EP - 9 JF - Acta poloniae pharmaceutica JO - Acta Pol Pharm VL - 68 IS - 1 N2 - This study was undertaken to assess in vitro lipid peroxidation inhibitions and anti-radical activities of methanolic, chloroform, ethyl acetate and water fractions of Mangifera indica leaf. Inhibition of Fe(2+)-induced lipid peroxidation (LPO) in egg, brain, and liver homogenates, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl (OH-) radical scavenging activities were evaluated. Total phenol was assessed in all fractions, and the reducing power of methanolic fraction was compared to gallic acid and ascorbic acid. The results showed that Fe2+ induced significant lipid peroxidation (LPO) in all the homogenates. Ethyl acetate fraction showed the highest percentage inhibition of LPO in both egg yolk (68.3%) and brain (66.3%), while the aqueous fraction exerted the highest inhibition in liver homogenate (89.1%) at a concentration of 10 microg/mL. These observed inhibitions of LPO by these fractions were higher than that of ascorbic acid used as a standard. The DPPH radical scavenging ability exhibited by ethyl acetate fraction was found to be the highest with IC50 value of 1.5 microg/mL. The ethyl acetate and methanolic fractions had the highest OH- radical scavenging ability with the same IC50 value of 5 microg/mL. The total phenol content of ethyl acetate fraction was the highest with 0.127 microg/mg gallic acid equivalent (GAE). The reductive potential of methanolic fraction showed a concentration-dependent increase. This study showed that inhibition of LPO and the DPPH and OH- radicals scavenging abilities of Mangifera indica leaf could be related to the presence of phenolic compounds. Therefore, the ethyl acetate fraction of the leaf may be a good source of natural antioxidative agent. SN - 0001-6837 UR - https://www.unboundmedicine.com/medline/citation/21485698/Lipid_peroxidation_inhibition_and_antiradical_activities_of_some_leaf_fractions_of_Mangifera_indica_ L2 - http://www.ptfarm.pl/pub/File/Acta_Poloniae/2011/1/023.pdf DB - PRIME DP - Unbound Medicine ER -