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Stability of ceftazidime in 1% and 5% buffered eye drops determined with HPLC method.
Acta Pol Pharm. 2011 Jan-Feb; 68(1):99-107.AP

Abstract

The aim of the studies was to determine with HPLC method the stability of ceftazidime in buffered 1% and 5% eye drops of proposed formulary composition, which were stored for 30 days at the temperature of 4 degrees C and 20 degrees C and protected from light. The 1% and 5% eye drops were prepared under aseptic conditions by dissolving Biotum (ceftazidimum), dry injection formulation, in citrate buffer of pH 6.10-6.24. The viscosity of the eye drops was increased with polyvinyl alcohol, phenylmercuric borate combined with 2-phenylethanol was used to preserve the eye drops. The eye drops were stored for 30 days in sterile glass bottles at the temperature of 4 degrees C and 20 degrees C and protected from light. The concentration of ceftazidime and pyridine was analyzed simultaneously with HPLC method every three days; pH, osmotic pressure and viscosity were examined as well as the organoleptic analysis of the eye drops (clarity, color, odor). Storage temperature had the biggest impact on ceftazidime stability in the eye drops. The stability of the drops depended also on ceftazidime concentration in the eye drops, the presence of preservatives and polyvinyl alcohol. The time of 10% ceftazidime degradation in buffered 1% and 5% eye drops, stored at the temperature of 4 degrees C, was from 27 to 18 days in 1% eye drops and from 21 to 12 days in 5% eye drops, depending on their composition. In the eye drops which were stored at the temperature of 20 degrees C 10% ceftazidime degradation occurred on the 3rd day of storage in all 1% and 5% formulary versions.

Authors+Show Affiliations

Department of Pharmaceutical Technology, Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, M. Skłodowskiej-Curie 9, Bydgoszcz, Poland. anna.kodym@cm.umk.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21485707

Citation

Kodym, Anna, et al. "Stability of Ceftazidime in 1% and 5% Buffered Eye Drops Determined With HPLC Method." Acta Poloniae Pharmaceutica, vol. 68, no. 1, 2011, pp. 99-107.
Kodym A, Hapka-Zmich D, Gołab M, et al. Stability of ceftazidime in 1% and 5% buffered eye drops determined with HPLC method. Acta Pol Pharm. 2011;68(1):99-107.
Kodym, A., Hapka-Zmich, D., Gołab, M., & Gwizdala, M. (2011). Stability of ceftazidime in 1% and 5% buffered eye drops determined with HPLC method. Acta Poloniae Pharmaceutica, 68(1), 99-107.
Kodym A, et al. Stability of Ceftazidime in 1% and 5% Buffered Eye Drops Determined With HPLC Method. Acta Pol Pharm. 2011 Jan-Feb;68(1):99-107. PubMed PMID: 21485707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of ceftazidime in 1% and 5% buffered eye drops determined with HPLC method. AU - Kodym,Anna, AU - Hapka-Zmich,Dominika, AU - Gołab,Marta, AU - Gwizdala,Magdalena, PY - 2011/4/14/entrez PY - 2011/4/14/pubmed PY - 2011/5/12/medline SP - 99 EP - 107 JF - Acta poloniae pharmaceutica JO - Acta Pol Pharm VL - 68 IS - 1 N2 - The aim of the studies was to determine with HPLC method the stability of ceftazidime in buffered 1% and 5% eye drops of proposed formulary composition, which were stored for 30 days at the temperature of 4 degrees C and 20 degrees C and protected from light. The 1% and 5% eye drops were prepared under aseptic conditions by dissolving Biotum (ceftazidimum), dry injection formulation, in citrate buffer of pH 6.10-6.24. The viscosity of the eye drops was increased with polyvinyl alcohol, phenylmercuric borate combined with 2-phenylethanol was used to preserve the eye drops. The eye drops were stored for 30 days in sterile glass bottles at the temperature of 4 degrees C and 20 degrees C and protected from light. The concentration of ceftazidime and pyridine was analyzed simultaneously with HPLC method every three days; pH, osmotic pressure and viscosity were examined as well as the organoleptic analysis of the eye drops (clarity, color, odor). Storage temperature had the biggest impact on ceftazidime stability in the eye drops. The stability of the drops depended also on ceftazidime concentration in the eye drops, the presence of preservatives and polyvinyl alcohol. The time of 10% ceftazidime degradation in buffered 1% and 5% eye drops, stored at the temperature of 4 degrees C, was from 27 to 18 days in 1% eye drops and from 21 to 12 days in 5% eye drops, depending on their composition. In the eye drops which were stored at the temperature of 20 degrees C 10% ceftazidime degradation occurred on the 3rd day of storage in all 1% and 5% formulary versions. SN - 0001-6837 UR - https://www.unboundmedicine.com/medline/citation/21485707/Stability_of_ceftazidime_in_1_and_5_buffered_eye_drops_determined_with_HPLC_method_ L2 - http://www.ptfarm.pl/pub/File/Acta_Poloniae/2011/1/099.pdf DB - PRIME DP - Unbound Medicine ER -