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Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors.
BMC Microbiol 2011; 11:72BM

Abstract

BACKGROUND

SecDF is an accessory factor of the conserved Sec protein translocation machinery and belongs to the resistance-nodulation-cell division (RND) family of multidrug exporters. SecDF has been shown in Escherichia coli and Bacillus subtilis to be involved in the export of proteins. RND proteins can mediate resistance against various substances and might be of relevance in antimicrobial therapy. The role of RND proteins in Staphylococcus aureus has not yet been determined.

RESULTS

Markerless deletion mutants were constructed to analyze the impact of the so far uncharacterized RND proteins in S. aureus. While the lack of Sa2056 and Sa2339 caused no phenotype regarding growth and resistance, the secDF mutant resulted in a pleiotropic phenotype. The secDF mutant was cold sensitive, but grew normally in rich medium at 37°C. Resistance to beta-lactams, glycopeptides and the RND substrates acriflavine, ethidium bromide and sodium dodecyl sulfate was reduced. The secDF mutant showed an aberrant cell separation and increased spontaneous and Triton X-100 induced autolysis, although the amounts of penicillin-binding proteins in the membrane were unchanged. The impact of secDF deletion on transcription and expression of specific virulence determinants varied: While coagulase transcription and activity were reduced, the opposite was observed for the autolysin Atl. A reduction of the transcription of the cell wall anchored protein A (spa) was also found. The accumulation of SpA in the membrane and lowered amounts in the cell wall pointed to an impaired translocation.

CONCLUSIONS

The combination of different effects of secDF deletion on transcription, regulation and translocation lead to impaired cell division, reduced resistance and altered expression of virulence determinants suggesting SecDF to be of major relevance in S. aureus. Thus SecDF could be a potential target for the control and eradication of S. aureus in the future.

Authors+Show Affiliations

Institute of Medical Microbiology, University of Zurich, Gloriastr 32, 8006 Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21486434

Citation

Quiblier, Chantal, et al. "Contribution of SecDF to Staphylococcus Aureus Resistance and Expression of Virulence Factors." BMC Microbiology, vol. 11, 2011, p. 72.
Quiblier C, Zinkernagel AS, Schuepbach RA, et al. Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors. BMC Microbiol. 2011;11:72.
Quiblier, C., Zinkernagel, A. S., Schuepbach, R. A., Berger-Bächi, B., & Senn, M. M. (2011). Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors. BMC Microbiology, 11, p. 72. doi:10.1186/1471-2180-11-72.
Quiblier C, et al. Contribution of SecDF to Staphylococcus Aureus Resistance and Expression of Virulence Factors. BMC Microbiol. 2011 Apr 12;11:72. PubMed PMID: 21486434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Contribution of SecDF to Staphylococcus aureus resistance and expression of virulence factors. AU - Quiblier,Chantal, AU - Zinkernagel,Annelies S, AU - Schuepbach,Reto A, AU - Berger-Bächi,Brigitte, AU - Senn,Maria M, Y1 - 2011/04/12/ PY - 2011/01/20/received PY - 2011/04/12/accepted PY - 2011/4/14/entrez PY - 2011/4/14/pubmed PY - 2011/7/26/medline SP - 72 EP - 72 JF - BMC microbiology JO - BMC Microbiol. VL - 11 N2 - BACKGROUND: SecDF is an accessory factor of the conserved Sec protein translocation machinery and belongs to the resistance-nodulation-cell division (RND) family of multidrug exporters. SecDF has been shown in Escherichia coli and Bacillus subtilis to be involved in the export of proteins. RND proteins can mediate resistance against various substances and might be of relevance in antimicrobial therapy. The role of RND proteins in Staphylococcus aureus has not yet been determined. RESULTS: Markerless deletion mutants were constructed to analyze the impact of the so far uncharacterized RND proteins in S. aureus. While the lack of Sa2056 and Sa2339 caused no phenotype regarding growth and resistance, the secDF mutant resulted in a pleiotropic phenotype. The secDF mutant was cold sensitive, but grew normally in rich medium at 37°C. Resistance to beta-lactams, glycopeptides and the RND substrates acriflavine, ethidium bromide and sodium dodecyl sulfate was reduced. The secDF mutant showed an aberrant cell separation and increased spontaneous and Triton X-100 induced autolysis, although the amounts of penicillin-binding proteins in the membrane were unchanged. The impact of secDF deletion on transcription and expression of specific virulence determinants varied: While coagulase transcription and activity were reduced, the opposite was observed for the autolysin Atl. A reduction of the transcription of the cell wall anchored protein A (spa) was also found. The accumulation of SpA in the membrane and lowered amounts in the cell wall pointed to an impaired translocation. CONCLUSIONS: The combination of different effects of secDF deletion on transcription, regulation and translocation lead to impaired cell division, reduced resistance and altered expression of virulence determinants suggesting SecDF to be of major relevance in S. aureus. Thus SecDF could be a potential target for the control and eradication of S. aureus in the future. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/21486434/Contribution_of_SecDF_to_Staphylococcus_aureus_resistance_and_expression_of_virulence_factors_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-11-72 DB - PRIME DP - Unbound Medicine ER -