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Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease.
Immunology. 1990 Dec; 71(4):460-6.I

Abstract

Expression of CD45RO on intraepithelial lymphocytes (IEL) bearing the T-cell receptor (TcR) alpha/beta or gamma/delta was studied in situ by three-colour immunofluorescence on jejunal tissue sections from 21 patients with coeliac disease and eight controls. CD45RA-TcR alpha/beta+ IEL expressed CD45RO significantly more often (75%) than the preferentially expanded TcR gamma/delta+ counterpart (59%). Triple staining for CD3, CD4/8 and CD45RA or CD45RB revealed that all CD3 + 4 - 8 - IEL (taken to be TcR gamma/delta+) expressed CD45RB and none were CD45RA. CD45RO positivity was of the same magnitude (66%) on the predominating monoclonal antibody delta TCS1-reactive fraction of TcR gamma/delta+ cells as on the remainder of the TcR gamma/delta+ subset. These results suggest that gluten exposition in patients with coeliac disease leads to accumulation of CD45RA-, putative antigen-primed memory cells of both TcR phenotypes. The less marked CD45RO expression within the preferentially expanded TcR gamma/delta+ subset of IEL may be of particular biological interest.

Authors+Show Affiliations

Institute of Pathology, University of Oslo, National Hospital, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2149120

Citation

Halstensen, T S., et al. "Intraepithelial TcR Alpha/beta+ Lymphocytes Express CD45RO More Often Than the TcR Gamma/delta+ Counterparts in Coeliac Disease." Immunology, vol. 71, no. 4, 1990, pp. 460-6.
Halstensen TS, Farstad IN, Scott H, et al. Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease. Immunology. 1990;71(4):460-6.
Halstensen, T. S., Farstad, I. N., Scott, H., Fausa, O., & Brandtzaeg, P. (1990). Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease. Immunology, 71(4), 460-6.
Halstensen TS, et al. Intraepithelial TcR Alpha/beta+ Lymphocytes Express CD45RO More Often Than the TcR Gamma/delta+ Counterparts in Coeliac Disease. Immunology. 1990;71(4):460-6. PubMed PMID: 2149120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease. AU - Halstensen,T S, AU - Farstad,I N, AU - Scott,H, AU - Fausa,O, AU - Brandtzaeg,P, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 460 EP - 6 JF - Immunology JO - Immunology VL - 71 IS - 4 N2 - Expression of CD45RO on intraepithelial lymphocytes (IEL) bearing the T-cell receptor (TcR) alpha/beta or gamma/delta was studied in situ by three-colour immunofluorescence on jejunal tissue sections from 21 patients with coeliac disease and eight controls. CD45RA-TcR alpha/beta+ IEL expressed CD45RO significantly more often (75%) than the preferentially expanded TcR gamma/delta+ counterpart (59%). Triple staining for CD3, CD4/8 and CD45RA or CD45RB revealed that all CD3 + 4 - 8 - IEL (taken to be TcR gamma/delta+) expressed CD45RB and none were CD45RA. CD45RO positivity was of the same magnitude (66%) on the predominating monoclonal antibody delta TCS1-reactive fraction of TcR gamma/delta+ cells as on the remainder of the TcR gamma/delta+ subset. These results suggest that gluten exposition in patients with coeliac disease leads to accumulation of CD45RA-, putative antigen-primed memory cells of both TcR phenotypes. The less marked CD45RO expression within the preferentially expanded TcR gamma/delta+ subset of IEL may be of particular biological interest. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/2149120/Intraepithelial_TcR_alpha/beta+_lymphocytes_express_CD45RO_more_often_than_the_TcR_gamma/delta+_counterparts_in_coeliac_disease_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/2149120/ DB - PRIME DP - Unbound Medicine ER -