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Headspace solid-phase microextraction combined with GC×GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes.
J Sep Sci. 2011 May; 34(10):1157-66.JS

Abstract

In this study, the investigation of the volatile compounds of dried rhizomes of Coptis chinensis Franch, C. deltoidea C. Y. Cheng et Hsiao, and C. teeta Wall was carried out to complete the chemical composition of these valuable natural products. Volatile profiles were established and compared after headspace solid-phase microextraction (HS-SPME) using a polydimethylsiloxane/divinylbenzene (PDMS/DVB, 65 μm) fibre coupled to comprehensive 2D gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS). Analyses were performed and compared on two column-phase combinations (non-polar/polar and polar/non-polar). The majority of the identified compounds eluted as well-separated (pure) components as a result of high-resolution capability of the GC×GC method, which significantly reduces co-elution. Therefore, this increases the likelihood that pure mass spectra can be obtained. More than 290 volatile and semi-volatile organic compounds were tentatively characterized by means of GC×GC in tandem with TOFMS detection. Improved result interpretations were obtained in terms of compound classification based on the organized structure of the peaks of structurally related compounds in the GC×GC contour plot. These compounds are distributed over the chemical groups of hydrocarbons, acids, alkenes, alkynes, aldehydes, ketones, alcohols, esters, furans, and terpenoids. Among all the chemical groups, terpenoids present the higher number of identified compounds (44), alkenes (41), and aldehydes and ketones (28). This study completed the volatile phytochemical analysis of the headspace composition of various Coptis species rhizomes, and should serve as a means to identify the difference between the rhizomes and may also be useful to confirm individual species based on their volatile chemical profile.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gao X
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Health Science Centre, Peking University, Beijing, P. R. China.
Yang X
No affiliation info available
Mitrevski BS
No affiliation info available
Marriott PJ
No affiliation info available

MeSH

CoptisDrugs, Chinese HerbalGas Chromatography-Mass SpectrometrySolid Phase MicroextractionVolatile Organic Compounds

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21491598

Citation

Gao, Xin, et al. "Headspace Solid-phase Microextraction Combined With GC×GC-TOFMS for the Analysis of Volatile Compounds of Coptis Species Rhizomes." Journal of Separation Science, vol. 34, no. 10, 2011, pp. 1157-66.
Gao X, Yang X, Mitrevski BS, et al. Headspace solid-phase microextraction combined with GC×GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes. J Sep Sci. 2011;34(10):1157-66.
Gao, X., Yang, X., Mitrevski, B. S., & Marriott, P. J. (2011). Headspace solid-phase microextraction combined with GC×GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes. Journal of Separation Science, 34(10), 1157-66. https://doi.org/10.1002/jssc.201100022
Gao X, et al. Headspace Solid-phase Microextraction Combined With GC×GC-TOFMS for the Analysis of Volatile Compounds of Coptis Species Rhizomes. J Sep Sci. 2011;34(10):1157-66. PubMed PMID: 21491598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Headspace solid-phase microextraction combined with GC×GC-TOFMS for the analysis of volatile compounds of Coptis species rhizomes. AU - Gao,Xin, AU - Yang,Xiuwei, AU - Mitrevski,Blagoj S, AU - Marriott,Philip J, Y1 - 2011/04/13/ PY - 2011/01/10/received PY - 2011/02/15/revised PY - 2011/02/17/accepted PY - 2011/4/15/entrez PY - 2011/4/15/pubmed PY - 2011/8/31/medline SP - 1157 EP - 66 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 10 N2 - In this study, the investigation of the volatile compounds of dried rhizomes of Coptis chinensis Franch, C. deltoidea C. Y. Cheng et Hsiao, and C. teeta Wall was carried out to complete the chemical composition of these valuable natural products. Volatile profiles were established and compared after headspace solid-phase microextraction (HS-SPME) using a polydimethylsiloxane/divinylbenzene (PDMS/DVB, 65 μm) fibre coupled to comprehensive 2D gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS). Analyses were performed and compared on two column-phase combinations (non-polar/polar and polar/non-polar). The majority of the identified compounds eluted as well-separated (pure) components as a result of high-resolution capability of the GC×GC method, which significantly reduces co-elution. Therefore, this increases the likelihood that pure mass spectra can be obtained. More than 290 volatile and semi-volatile organic compounds were tentatively characterized by means of GC×GC in tandem with TOFMS detection. Improved result interpretations were obtained in terms of compound classification based on the organized structure of the peaks of structurally related compounds in the GC×GC contour plot. These compounds are distributed over the chemical groups of hydrocarbons, acids, alkenes, alkynes, aldehydes, ketones, alcohols, esters, furans, and terpenoids. Among all the chemical groups, terpenoids present the higher number of identified compounds (44), alkenes (41), and aldehydes and ketones (28). This study completed the volatile phytochemical analysis of the headspace composition of various Coptis species rhizomes, and should serve as a means to identify the difference between the rhizomes and may also be useful to confirm individual species based on their volatile chemical profile. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21491598/Headspace_solid_phase_microextraction_combined_with_GC×GC_TOFMS_for_the_analysis_of_volatile_compounds_of_Coptis_species_rhizomes_ L2 - https://doi.org/10.1002/jssc.201100022 DB - PRIME DP - Unbound Medicine ER -
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