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Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters.
Syst Appl Microbiol. 2011 Jun; 34(4):303-10.SA

Abstract

This study presents a genetic characterization of 27 potentially toxic cyanobacterial strains isolated from seven reservoirs located in the north and centre of Tunisia. These strains belonged mainly to Microcystis aeruginosa, Cylindrospermopsis raciborskii and Planktothrix agardhii species. Their toxicological potential was evaluated by molecular biology tools, which showed that none of the isolated strains carried segments of the gene cluster responsible for the production of cylindrospermopsin and saxitoxin. The majority of Microcystis isolates were able to synthesize microcystin, since they presented the six characteristic segments of the microcystin synthetase mcy cluster (mcyA, -B, -C, -D, -E and -G). This was further confirmed by MALDI-TOF analysis that showed the presence of eight microcystin variants, including microcystin-LR. The taxonomic identification of the strains was assessed based on the variability of the 16S rRNA gene sequences. Furthermore, the 16S-23S rRNA ITS sequences of Microcystis isolates and rpoC1 sequences of Cylindrospermopsis strains were also used in the phylogenetic analysis.

Authors+Show Affiliations

Unité de Recherche Ecosystèmes et Ressources Aquatiques, Institut National Agronomique de Tunisie, Tunis Mahrajène, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21493030

Citation

Fathalli, Afef, et al. "Molecular and Phylogenetic Characterization of Potentially Toxic Cyanobacteria in Tunisian Freshwaters." Systematic and Applied Microbiology, vol. 34, no. 4, 2011, pp. 303-10.
Fathalli A, Jenhani AB, Moreira C, et al. Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters. Syst Appl Microbiol. 2011;34(4):303-10.
Fathalli, A., Jenhani, A. B., Moreira, C., Welker, M., Romdhane, M., Antunes, A., & Vasconcelos, V. (2011). Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters. Systematic and Applied Microbiology, 34(4), 303-10. https://doi.org/10.1016/j.syapm.2010.12.003
Fathalli A, et al. Molecular and Phylogenetic Characterization of Potentially Toxic Cyanobacteria in Tunisian Freshwaters. Syst Appl Microbiol. 2011;34(4):303-10. PubMed PMID: 21493030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters. AU - Fathalli,Afef, AU - Jenhani,Amel Ben Rejeb, AU - Moreira,Cristiana, AU - Welker,Martin, AU - Romdhane,Mohamed, AU - Antunes,Agostinho, AU - Vasconcelos,Vitor, Y1 - 2011/04/13/ PY - 2010/06/28/received PY - 2010/12/17/revised PY - 2010/12/17/accepted PY - 2011/4/16/entrez PY - 2011/4/16/pubmed PY - 2011/8/13/medline SP - 303 EP - 10 JF - Systematic and applied microbiology JO - Syst Appl Microbiol VL - 34 IS - 4 N2 - This study presents a genetic characterization of 27 potentially toxic cyanobacterial strains isolated from seven reservoirs located in the north and centre of Tunisia. These strains belonged mainly to Microcystis aeruginosa, Cylindrospermopsis raciborskii and Planktothrix agardhii species. Their toxicological potential was evaluated by molecular biology tools, which showed that none of the isolated strains carried segments of the gene cluster responsible for the production of cylindrospermopsin and saxitoxin. The majority of Microcystis isolates were able to synthesize microcystin, since they presented the six characteristic segments of the microcystin synthetase mcy cluster (mcyA, -B, -C, -D, -E and -G). This was further confirmed by MALDI-TOF analysis that showed the presence of eight microcystin variants, including microcystin-LR. The taxonomic identification of the strains was assessed based on the variability of the 16S rRNA gene sequences. Furthermore, the 16S-23S rRNA ITS sequences of Microcystis isolates and rpoC1 sequences of Cylindrospermopsis strains were also used in the phylogenetic analysis. SN - 1618-0984 UR - https://www.unboundmedicine.com/medline/citation/21493030/Molecular_and_phylogenetic_characterization_of_potentially_toxic_cyanobacteria_in_Tunisian_freshwaters_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0723-2020(11)00057-9 DB - PRIME DP - Unbound Medicine ER -