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Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
Eur J Pharmacol. 2011 Jun 25; 660(2-3):283-90.EJ

Abstract

1-methyl-4-phenylpyridinium (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin for inducing a cell model of Parkinson's disease. This study aimed to evaluate the effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, on MPP+-induced cytotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. The results from the MTT and lactate dehydrogenase (LDH) assays showed that incubating cells with 500 μM MPP+ for 24 h decreased cell viability and increased LDH leakage, whereas preincubating cells with 3.125 to 50 μM TSG for 24 h protected the cells against MPP+-induced cell damage. Using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and rhodamine 123, respectively, we found that TSG inhibited both the elevation of intracellular reactive oxygen species and the disruption of mitochondrial membrane potential induced by MPP+. In addition, TSG suppressed both the upregulation of the ratio of Bax to Bcl-2 and the activation of caspase-3 induced by MPP+, and TSG inhibited apoptosis as detected by flow cytometric analysis using Annexin-V and propidium (PI) label. These results suggest that TSG may protect neurons against MPP+-induced cell death through improving mitochondrial function, decreasing oxidative stress and inhibiting apoptosis, and this may provide a potentially new strategy for preventing and treating neurodegenerative disorders such as Parkinson's disease.

Authors+Show Affiliations

Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21497157

Citation

Sun, Fang-ling, et al. "Tetrahydroxystilbene Glucoside Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity." European Journal of Pharmacology, vol. 660, no. 2-3, 2011, pp. 283-90.
Sun FL, Zhang L, Zhang RY, et al. Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Eur J Pharmacol. 2011;660(2-3):283-90.
Sun, F. L., Zhang, L., Zhang, R. Y., & Li, L. (2011). Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. European Journal of Pharmacology, 660(2-3), 283-90. https://doi.org/10.1016/j.ejphar.2011.03.046
Sun FL, et al. Tetrahydroxystilbene Glucoside Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity. Eur J Pharmacol. 2011 Jun 25;660(2-3):283-90. PubMed PMID: 21497157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. AU - Sun,Fang-ling, AU - Zhang,Lan, AU - Zhang,Ru-yi, AU - Li,Lin, Y1 - 2011/04/09/ PY - 2010/11/14/received PY - 2011/03/05/revised PY - 2011/03/21/accepted PY - 2011/4/19/entrez PY - 2011/4/19/pubmed PY - 2011/9/7/medline SP - 283 EP - 90 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 660 IS - 2-3 N2 - 1-methyl-4-phenylpyridinium (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin for inducing a cell model of Parkinson's disease. This study aimed to evaluate the effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, on MPP+-induced cytotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. The results from the MTT and lactate dehydrogenase (LDH) assays showed that incubating cells with 500 μM MPP+ for 24 h decreased cell viability and increased LDH leakage, whereas preincubating cells with 3.125 to 50 μM TSG for 24 h protected the cells against MPP+-induced cell damage. Using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and rhodamine 123, respectively, we found that TSG inhibited both the elevation of intracellular reactive oxygen species and the disruption of mitochondrial membrane potential induced by MPP+. In addition, TSG suppressed both the upregulation of the ratio of Bax to Bcl-2 and the activation of caspase-3 induced by MPP+, and TSG inhibited apoptosis as detected by flow cytometric analysis using Annexin-V and propidium (PI) label. These results suggest that TSG may protect neurons against MPP+-induced cell death through improving mitochondrial function, decreasing oxidative stress and inhibiting apoptosis, and this may provide a potentially new strategy for preventing and treating neurodegenerative disorders such as Parkinson's disease. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21497157/Tetrahydroxystilbene_glucoside_protects_human_neuroblastoma_SH_SY5Y_cells_against_MPP+_induced_cytotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)00362-1 DB - PRIME DP - Unbound Medicine ER -