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Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity.
Eur J Pharmacol. 2011 Jun 25; 660(2-3):283-90.EJ

Abstract

1-methyl-4-phenylpyridinium (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin for inducing a cell model of Parkinson's disease. This study aimed to evaluate the effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, on MPP+-induced cytotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. The results from the MTT and lactate dehydrogenase (LDH) assays showed that incubating cells with 500 μM MPP+ for 24 h decreased cell viability and increased LDH leakage, whereas preincubating cells with 3.125 to 50 μM TSG for 24 h protected the cells against MPP+-induced cell damage. Using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and rhodamine 123, respectively, we found that TSG inhibited both the elevation of intracellular reactive oxygen species and the disruption of mitochondrial membrane potential induced by MPP+. In addition, TSG suppressed both the upregulation of the ratio of Bax to Bcl-2 and the activation of caspase-3 induced by MPP+, and TSG inhibited apoptosis as detected by flow cytometric analysis using Annexin-V and propidium (PI) label. These results suggest that TSG may protect neurons against MPP+-induced cell death through improving mitochondrial function, decreasing oxidative stress and inhibiting apoptosis, and this may provide a potentially new strategy for preventing and treating neurodegenerative disorders such as Parkinson's disease.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sun FL
Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China.
Zhang L
No affiliation info available
Zhang RY
No affiliation info available
Li L
No affiliation info available

MeSH

1-Methyl-4-phenylpyridiniumCaspase 3Cell Line, TumorCell MembraneCell SurvivalCytoprotectionGene Expression RegulationGlucosidesHumansMitochondriaNeuroblastomaNeuroprotective AgentsOxidative StressProto-Oncogene Proteins c-bcl-2Reactive Oxygen SpeciesStilbenesbcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21497157

Citation

Sun, Fang-ling, et al. "Tetrahydroxystilbene Glucoside Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity." European Journal of Pharmacology, vol. 660, no. 2-3, 2011, pp. 283-90.
Sun FL, Zhang L, Zhang RY, et al. Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. Eur J Pharmacol. 2011;660(2-3):283-90.
Sun, F. L., Zhang, L., Zhang, R. Y., & Li, L. (2011). Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. European Journal of Pharmacology, 660(2-3), 283-90. https://doi.org/10.1016/j.ejphar.2011.03.046
Sun FL, et al. Tetrahydroxystilbene Glucoside Protects Human Neuroblastoma SH-SY5Y Cells Against MPP+-induced Cytotoxicity. Eur J Pharmacol. 2011 Jun 25;660(2-3):283-90. PubMed PMID: 21497157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity. AU - Sun,Fang-ling, AU - Zhang,Lan, AU - Zhang,Ru-yi, AU - Li,Lin, Y1 - 2011/04/09/ PY - 2010/11/14/received PY - 2011/03/05/revised PY - 2011/03/21/accepted PY - 2011/4/19/entrez PY - 2011/4/19/pubmed PY - 2011/9/7/medline SP - 283 EP - 90 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 660 IS - 2-3 N2 - 1-methyl-4-phenylpyridinium (MPP+), an inhibitor of mitochondrial complex I, has been widely used as a neurotoxin for inducing a cell model of Parkinson's disease. This study aimed to evaluate the effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, on MPP+-induced cytotoxicity in human dopaminergic neuroblastoma SH-SY5Y cells. The results from the MTT and lactate dehydrogenase (LDH) assays showed that incubating cells with 500 μM MPP+ for 24 h decreased cell viability and increased LDH leakage, whereas preincubating cells with 3.125 to 50 μM TSG for 24 h protected the cells against MPP+-induced cell damage. Using 2',7'-dichlorofluorescin diacetate (DCFH-DA) and rhodamine 123, respectively, we found that TSG inhibited both the elevation of intracellular reactive oxygen species and the disruption of mitochondrial membrane potential induced by MPP+. In addition, TSG suppressed both the upregulation of the ratio of Bax to Bcl-2 and the activation of caspase-3 induced by MPP+, and TSG inhibited apoptosis as detected by flow cytometric analysis using Annexin-V and propidium (PI) label. These results suggest that TSG may protect neurons against MPP+-induced cell death through improving mitochondrial function, decreasing oxidative stress and inhibiting apoptosis, and this may provide a potentially new strategy for preventing and treating neurodegenerative disorders such as Parkinson's disease. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21497157/Tetrahydroxystilbene_glucoside_protects_human_neuroblastoma_SH_SY5Y_cells_against_MPP+_induced_cytotoxicity_ DB - PRIME DP - Unbound Medicine ER -