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HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.
J Pharm Biomed Anal. 2011 Jul 15; 55(5):934-48.JP

Abstract

In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis C(18) column (250mm×4.6mm I.D., 5μm), with a mobile phase composed by 0.1% formic acid in water and acetonitrile. Overall, the UV spectra, the MS and MS/MS data allowed the identification of 40 compounds. In the case of flavonoids, the triple quadrupole mass analyzer provided more collision energy if compared with the ion trap, originating product ions at best sensitivity. The HPLC method was validated in agreement with ICH guidelines: the correlation coefficients were >0.998; the limit of detection was in the range 1.6-4.6μg/ml; the recovery range was 96-105%; the intra- and inter-day %RSD values for retention times and peak areas were found to be <0.3 and 1.9%, respectively. The developed technique was applied to the analysis of hydroalcoholic extracts of propolis available on the Italian market. Although the chromatographic profile of the analyzed samples was similar, the quantitative analysis indicated that there is a great variability in the amount of the active compounds: the content of total phenolic acids ranged from 0.17 to 16.67mg/ml and the level of total flavonoids from 2.48 to 41.10mg/ml. The proposed method can be considered suitable for the phytochemical analysis of propolis extracts used in phytotherapy.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Via G. Campi 183, 41125 Modena, Italy. federica.pellati@unimore.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21497475

Citation

Pellati, Federica, et al. "HPLC-DAD and HPLC-ESI-MS/MS Methods for Metabolite Profiling of Propolis Extracts." Journal of Pharmaceutical and Biomedical Analysis, vol. 55, no. 5, 2011, pp. 934-48.
Pellati F, Orlandini G, Pinetti D, et al. HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. J Pharm Biomed Anal. 2011;55(5):934-48.
Pellati, F., Orlandini, G., Pinetti, D., & Benvenuti, S. (2011). HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. Journal of Pharmaceutical and Biomedical Analysis, 55(5), 934-48. https://doi.org/10.1016/j.jpba.2011.03.024
Pellati F, et al. HPLC-DAD and HPLC-ESI-MS/MS Methods for Metabolite Profiling of Propolis Extracts. J Pharm Biomed Anal. 2011 Jul 15;55(5):934-48. PubMed PMID: 21497475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts. AU - Pellati,Federica, AU - Orlandini,Giulia, AU - Pinetti,Diego, AU - Benvenuti,Stefania, Y1 - 2011/03/25/ PY - 2010/11/26/received PY - 2011/03/16/revised PY - 2011/03/17/accepted PY - 2011/4/19/entrez PY - 2011/4/19/pubmed PY - 2011/9/20/medline SP - 934 EP - 48 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 55 IS - 5 N2 - In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis C(18) column (250mm×4.6mm I.D., 5μm), with a mobile phase composed by 0.1% formic acid in water and acetonitrile. Overall, the UV spectra, the MS and MS/MS data allowed the identification of 40 compounds. In the case of flavonoids, the triple quadrupole mass analyzer provided more collision energy if compared with the ion trap, originating product ions at best sensitivity. The HPLC method was validated in agreement with ICH guidelines: the correlation coefficients were >0.998; the limit of detection was in the range 1.6-4.6μg/ml; the recovery range was 96-105%; the intra- and inter-day %RSD values for retention times and peak areas were found to be <0.3 and 1.9%, respectively. The developed technique was applied to the analysis of hydroalcoholic extracts of propolis available on the Italian market. Although the chromatographic profile of the analyzed samples was similar, the quantitative analysis indicated that there is a great variability in the amount of the active compounds: the content of total phenolic acids ranged from 0.17 to 16.67mg/ml and the level of total flavonoids from 2.48 to 41.10mg/ml. The proposed method can be considered suitable for the phytochemical analysis of propolis extracts used in phytotherapy. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/21497475/HPLC_DAD_and_HPLC_ESI_MS/MS_methods_for_metabolite_profiling_of_propolis_extracts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(11)00158-0 DB - PRIME DP - Unbound Medicine ER -