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Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior.
Pancreas. 2011 May; 40(4):551-6.P

Abstract

OBJECTIVES

We examined the role of brain-derived neurotrophic factor (BDNF) in the pathogenesis of pain in an experimental model of chronic pancreatitis (CP).

METHODS

Pancreatitis was induced by retrograde infusion of trinitrobenzene sulfonic acid into the pancreatic duct of adult rats. Twenty-one days after injection, BDNF expression was examined in pancreas-specific dorsal root ganglia (DRGs) by immunohistochemistry, and protein levels were quantified from DRGs and spinal cord extracts. The effects of intrathecal infusion of a neutralizing antibody to BDNF on pancreatic hyperalgesia were assessed by the sensitivity of the abdominal wall to filament probing as well as the nocifensive behavior to electrical stimulation of the pancreas.

RESULTS

Levels of BDNF in DRGs and spinal cords (T9-13) were significantly higher in trinitrobenzene sulfonic acid rats compared with controls, accompanied by an increase in the number of pancreas-specific neurons expressing BDNF immunoreactivity. Brain-derived neurotrophic factor antagonism suppressed phospho-tropomyosin-related kinase B receptor levels in the spinal cord and significantly reduced behavioral responses in rats with CP.

CONCLUSIONS

Brain-derived neurotrophic factor is upregulated in pancreas-specific primary afferent neurons in rats with CP, and BDNF antagonism is associated with a reduction of pain-related behavior in these animals, suggesting an important role for this neurotransmitter in the nociception of CP.

Authors+Show Affiliations

Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21499209

Citation

Hughes, Michael S., et al. "Brain-derived Neurotrophic Factor Is Upregulated in Rats With Chronic Pancreatitis and Mediates Pain Behavior." Pancreas, vol. 40, no. 4, 2011, pp. 551-6.
Hughes MS, Shenoy M, Liu L, et al. Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. Pancreas. 2011;40(4):551-6.
Hughes, M. S., Shenoy, M., Liu, L., Colak, T., Mehta, K., & Pasricha, P. J. (2011). Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. Pancreas, 40(4), 551-6. https://doi.org/10.1097/MPA.0b013e318214fb77
Hughes MS, et al. Brain-derived Neurotrophic Factor Is Upregulated in Rats With Chronic Pancreatitis and Mediates Pain Behavior. Pancreas. 2011;40(4):551-6. PubMed PMID: 21499209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. AU - Hughes,Michael S, AU - Shenoy,Mohan, AU - Liu,Liansheng, AU - Colak,Tugba, AU - Mehta,Kshama, AU - Pasricha,Pankaj J, PY - 2011/4/19/entrez PY - 2011/4/19/pubmed PY - 2011/8/2/medline SP - 551 EP - 6 JF - Pancreas JO - Pancreas VL - 40 IS - 4 N2 - OBJECTIVES: We examined the role of brain-derived neurotrophic factor (BDNF) in the pathogenesis of pain in an experimental model of chronic pancreatitis (CP). METHODS: Pancreatitis was induced by retrograde infusion of trinitrobenzene sulfonic acid into the pancreatic duct of adult rats. Twenty-one days after injection, BDNF expression was examined in pancreas-specific dorsal root ganglia (DRGs) by immunohistochemistry, and protein levels were quantified from DRGs and spinal cord extracts. The effects of intrathecal infusion of a neutralizing antibody to BDNF on pancreatic hyperalgesia were assessed by the sensitivity of the abdominal wall to filament probing as well as the nocifensive behavior to electrical stimulation of the pancreas. RESULTS: Levels of BDNF in DRGs and spinal cords (T9-13) were significantly higher in trinitrobenzene sulfonic acid rats compared with controls, accompanied by an increase in the number of pancreas-specific neurons expressing BDNF immunoreactivity. Brain-derived neurotrophic factor antagonism suppressed phospho-tropomyosin-related kinase B receptor levels in the spinal cord and significantly reduced behavioral responses in rats with CP. CONCLUSIONS: Brain-derived neurotrophic factor is upregulated in pancreas-specific primary afferent neurons in rats with CP, and BDNF antagonism is associated with a reduction of pain-related behavior in these animals, suggesting an important role for this neurotransmitter in the nociception of CP. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/21499209/Brain_derived_neurotrophic_factor_is_upregulated_in_rats_with_chronic_pancreatitis_and_mediates_pain_behavior_ L2 - https://doi.org/10.1097/MPA.0b013e318214fb77 DB - PRIME DP - Unbound Medicine ER -