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Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods.
J Sep Sci. 2011 Jun; 34(11):1261-7.JS

Abstract

Comparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion (Allium cepa), parsley (Petroselinum crispum) roots and leaves, celery (Apium graveolens) roots and leaves and leaves of dill (Anethum graveolens) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) RSA of extracts with the results obtained using reversed-phase chromatographic separation with on-line post-column radical scavenging reaction detection, good correlation was obtained (R(2)=0.848). Studies using HPLC system with electrochemical detector showed that bioactive phytochemicals can be separated and antioxidant activities of individual compounds evaluated without the need of a complex HPLC system with reaction detector. The results obtained using electrochemical detection correlate with the RSA assayed using spectrophotometric method (R(2)=0.893).

Authors+Show Affiliations

Department of Biochemistry and Biotechnologies, Faculty of Natural Sciences, Vytautas Magnus University, Lithuania.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21504067

Citation

Stankevičius, Mantas, et al. "Comparative Analysis of Radical Scavenging and Antioxidant Activity of Phenolic Compounds Present in Everyday Use Spice Plants By Means of Spectrophotometric and Chromatographic Methods." Journal of Separation Science, vol. 34, no. 11, 2011, pp. 1261-7.
Stankevičius M, Akuņeca I, Jãkobsone I, et al. Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods. J Sep Sci. 2011;34(11):1261-7.
Stankevičius, M., Akuņeca, I., Jãkobsone, I., & Maruška, A. (2011). Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods. Journal of Separation Science, 34(11), 1261-7. https://doi.org/10.1002/jssc.201000915
Stankevičius M, et al. Comparative Analysis of Radical Scavenging and Antioxidant Activity of Phenolic Compounds Present in Everyday Use Spice Plants By Means of Spectrophotometric and Chromatographic Methods. J Sep Sci. 2011;34(11):1261-7. PubMed PMID: 21504067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods. AU - Stankevičius,Mantas, AU - Akuņeca,Ieva, AU - Jãkobsone,Ida, AU - Maruška,Audrius, Y1 - 2011/04/18/ PY - 2010/12/20/received PY - 2011/02/22/revised PY - 2011/03/08/accepted PY - 2011/4/20/entrez PY - 2011/4/20/pubmed PY - 2011/12/13/medline SP - 1261 EP - 7 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 11 N2 - Comparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion (Allium cepa), parsley (Petroselinum crispum) roots and leaves, celery (Apium graveolens) roots and leaves and leaves of dill (Anethum graveolens) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) RSA of extracts with the results obtained using reversed-phase chromatographic separation with on-line post-column radical scavenging reaction detection, good correlation was obtained (R(2)=0.848). Studies using HPLC system with electrochemical detector showed that bioactive phytochemicals can be separated and antioxidant activities of individual compounds evaluated without the need of a complex HPLC system with reaction detector. The results obtained using electrochemical detection correlate with the RSA assayed using spectrophotometric method (R(2)=0.893). SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21504067/Comparative_analysis_of_radical_scavenging_and_antioxidant_activity_of_phenolic_compounds_present_in_everyday_use_spice_plants_by_means_of_spectrophotometric_and_chromatographic_methods_ L2 - https://doi.org/10.1002/jssc.201000915 DB - PRIME DP - Unbound Medicine ER -