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Anti-inflammatory effects of the chloroform extract of Pulicaria guestii ameliorated the neutrophil infiltration and nitric oxide generation in rats.
Toxicol Ind Health. 2011 Nov; 27(10):899-910.TI

Abstract

Pulicaria guestii Rech.f. & Rawi is a fragrant, perennial herb, which grows wild, west of Al-Madinah, Saudi Arabia. Several reports were published on the anti-inflammatory activity of the sesquiterpene lactones, phenolics and flavonoids, which constitute the main active constituents of the members of the genus Pulicaria. The present study was designed to explore the potential anti-inflammatory effect of P. guestii in several experimental models. The methanol extract of the dried aerial parts of P. guestii was extracted with petroleum ether, chloroform and n-butanol. The chloroform extract was analysed on TLC and examined under UV and visible light in presence of AlCl(3) spray. The free radical scavenging activity and the total phenolic content in the CHCl(3) extract were estimated. The crude methanol extract and the CHCl(3) fraction were examined against carrageenin-induced paw edema and ear edema induced by croton oil application. The crude methanolic extract significantly reduced carrageenin-induced rat paw edema. After fractionation, the chloroform fraction caused significant reduction in carrageenin-induced rat paw edema in addition to diminishing prostaglandin E(2) (PGE(2)) in the inflammatory exudates. Topical application of chloroform fraction significantly reduced rat ear edema induced by croton oil application. In the same model, chloroform fraction reduced neutrophil infiltration, as indicated by the significant decrease in myeloperoxidase activity, and ameliorated histopathological changes induced by croton oil application. In lipopolysaccharide-induced inflammation in rat air pouch, chloroform fraction significantly reduced the nitric oxide level and tumor necrosis factor-α release. In conclusion, the chloroform fraction of P. guestii extract possesses anti-inflammatory activity in several experimental models. Further investigations are needed to identify the active constituents responsible for this anti-inflammatory activity.

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Authors+Show Affiliations

Alghaithy AA
Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawarah, Saudi Arabia.
El-Beshbishy HA
No affiliation info available
Abdel-Naim AB
No affiliation info available
Nagy AA
No affiliation info available
Abdel-Sattar EM
No affiliation info available

MeSH

AnimalsAnti-Inflammatory Agents, Non-SteroidalCarrageenanChemical FractionationDisease Models, AnimalEdemaFree Radical ScavengersHindlimbMaleNeutrophil InfiltrationNeutrophilsNitric OxidePhenolsPlant ExtractsPlant LeavesPulicariaRatsRats, Sprague-DawleyTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21505007

Citation

Alghaithy, A A., et al. "Anti-inflammatory Effects of the Chloroform Extract of Pulicaria Guestii Ameliorated the Neutrophil Infiltration and Nitric Oxide Generation in Rats." Toxicology and Industrial Health, vol. 27, no. 10, 2011, pp. 899-910.
Alghaithy AA, El-Beshbishy HA, Abdel-Naim AB, et al. Anti-inflammatory effects of the chloroform extract of Pulicaria guestii ameliorated the neutrophil infiltration and nitric oxide generation in rats. Toxicol Ind Health. 2011;27(10):899-910.
Alghaithy, A. A., El-Beshbishy, H. A., Abdel-Naim, A. B., Nagy, A. A., & Abdel-Sattar, E. M. (2011). Anti-inflammatory effects of the chloroform extract of Pulicaria guestii ameliorated the neutrophil infiltration and nitric oxide generation in rats. Toxicology and Industrial Health, 27(10), 899-910. https://doi.org/10.1177/0748233711399320
Alghaithy AA, et al. Anti-inflammatory Effects of the Chloroform Extract of Pulicaria Guestii Ameliorated the Neutrophil Infiltration and Nitric Oxide Generation in Rats. Toxicol Ind Health. 2011;27(10):899-910. PubMed PMID: 21505007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory effects of the chloroform extract of Pulicaria guestii ameliorated the neutrophil infiltration and nitric oxide generation in rats. AU - Alghaithy,A A, AU - El-Beshbishy,H A, AU - Abdel-Naim,A B, AU - Nagy,A A, AU - Abdel-Sattar,E M, Y1 - 2011/04/19/ PY - 2011/4/21/entrez PY - 2011/4/21/pubmed PY - 2012/3/9/medline SP - 899 EP - 910 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 27 IS - 10 N2 - Pulicaria guestii Rech.f. & Rawi is a fragrant, perennial herb, which grows wild, west of Al-Madinah, Saudi Arabia. Several reports were published on the anti-inflammatory activity of the sesquiterpene lactones, phenolics and flavonoids, which constitute the main active constituents of the members of the genus Pulicaria. The present study was designed to explore the potential anti-inflammatory effect of P. guestii in several experimental models. The methanol extract of the dried aerial parts of P. guestii was extracted with petroleum ether, chloroform and n-butanol. The chloroform extract was analysed on TLC and examined under UV and visible light in presence of AlCl(3) spray. The free radical scavenging activity and the total phenolic content in the CHCl(3) extract were estimated. The crude methanol extract and the CHCl(3) fraction were examined against carrageenin-induced paw edema and ear edema induced by croton oil application. The crude methanolic extract significantly reduced carrageenin-induced rat paw edema. After fractionation, the chloroform fraction caused significant reduction in carrageenin-induced rat paw edema in addition to diminishing prostaglandin E(2) (PGE(2)) in the inflammatory exudates. Topical application of chloroform fraction significantly reduced rat ear edema induced by croton oil application. In the same model, chloroform fraction reduced neutrophil infiltration, as indicated by the significant decrease in myeloperoxidase activity, and ameliorated histopathological changes induced by croton oil application. In lipopolysaccharide-induced inflammation in rat air pouch, chloroform fraction significantly reduced the nitric oxide level and tumor necrosis factor-α release. In conclusion, the chloroform fraction of P. guestii extract possesses anti-inflammatory activity in several experimental models. Further investigations are needed to identify the active constituents responsible for this anti-inflammatory activity. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/21505007/Anti_inflammatory_effects_of_the_chloroform_extract_of_Pulicaria_guestii_ameliorated_the_neutrophil_infiltration_and_nitric_oxide_generation_in_rats_ L2 - https://journals.sagepub.com/doi/10.1177/0748233711399320?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -