TY - JOUR T1 - Validation of an accelerated solvent extraction liquid chromatography-tandem mass spectrometry method for Pacific ciguatoxin-1 in fish flesh and comparison with the mouse neuroblastoma assay. AU - Wu,Jia Jun, AU - Mak,Yim Ling, AU - Murphy,Margaret B, AU - Lam,James C W, AU - Chan,Wing Hei, AU - Wang,Mingfu, AU - Chan,Leo L, AU - Lam,Paul K S, Y1 - 2011/04/21/ PY - 2011/01/16/received PY - 2011/03/31/accepted PY - 2011/03/22/revised PY - 2011/4/21/entrez PY - 2011/4/21/pubmed PY - 2011/9/29/medline SP - 3165 EP - 75 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 400 IS - 9 N2 - Ciguatera fish poisoning (CFP) is a global foodborne illness caused by consumption of seafood containing ciguatoxins (CTXs) originating from dinoflagellates such as Gambierdiscus toxicus. P-CTX-1 has been suggested to be the most toxic CTX, causing ciguatera at 0.1 μg/kg in the flesh of carnivorous fish. CTXs are structurally complex and difficult to quantify, but there is a need for analytical methods for CFP toxins in coral reef fishes to protect human health. In this paper, we describe a sensitive and rapid extraction method using accelerated solvent extraction combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the detection and quantification of P-CTX-1 in fish flesh. By the use of a more sensitive MS system (5500 QTRAP), the validated method has a limit of quantification (LOQ) of 0.01 μg/kg, linearity correlation coefficients above 0.99 for both solvent- and matrix-based standard solutions as well as matrix spike recoveries ranging from 49% to 85% in 17 coral reef fish species. Compared with previous methods, this method has better overall recovery, extraction efficiency and LOQ. Fish flesh from 12 blue-spotted groupers (Cephalopholis argus) was assessed for the presence of CTXs using HPLC-MS/MS analysis and the commonly used mouse neuroblastoma assay, and the results of the two methods were strongly correlated. This method is capable of detecting low concentrations of P-CTX-1 in fish at levels that are relevant to human health, making it suitable for monitoring of suspected ciguateric fish both in the environment and in the marketplace. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/21505950/Validation_of_an_accelerated_solvent_extraction_liquid_chromatography_tandem_mass_spectrometry_method_for_Pacific_ciguatoxin_1_in_fish_flesh_and_comparison_with_the_mouse_neuroblastoma_assay_ L2 - https://dx.doi.org/10.1007/s00216-011-4977-4 DB - PRIME DP - Unbound Medicine ER -