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Development of a double antibody sandwich ELISA assay for the diagnosis of angiostrongyliasis.
J Parasitol. 2011 Aug; 97(4):721-4.JP

Abstract

With the use of 2 monoclonal antibodies (MAbs) against excretory/secretory (ES) antigens of adult Angiostrongylus cantonensis, a new method was developed for double antibody sandwich ELISA for the detection of circulating antigens (CAg). To evaluate the sensitivity of the new procedure, the CAg in sera of rats (80) and mice (15) infected with A. cantonensis, as well as CAg in sera of clinically confirmed angiostrongyliasis patients (70), were evaluated. Cross-reaction testing was used to determine the specificity of serum from patients infected with Ascaris lumbricoides, Trichinella spiralis , Toxoplasma gondii , Schistosoma japonicum, Paragonimus westermani, Clonorchis sinensis, Echinococcus granulosus, Spirometra, and Taenia solium, as well as normal healthy people. The results proved that the sensitivity and the specificity of the new method were totally effective for the detection of A. cantonensis CAg. The assay is highly sensitive, specific, and reproducible, with easy handling and excellent cost effectiveness, and thereby provides a new method for the accurate diagnosis of angiostrongyliasis.

Authors+Show Affiliations

Shenzhen Center for Diseases Control and Prevention, Shenzhen, Guangdong Province 518020, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21506824

Citation

Chen, Mu-Xin, et al. "Development of a Double Antibody Sandwich ELISA Assay for the Diagnosis of Angiostrongyliasis." The Journal of Parasitology, vol. 97, no. 4, 2011, pp. 721-4.
Chen MX, Wang K, Ai L, et al. Development of a double antibody sandwich ELISA assay for the diagnosis of angiostrongyliasis. J Parasitol. 2011;97(4):721-4.
Chen, M. X., Wang, K., Ai, L., Yan, W. H., Peng, L., & Zhang, R. L. (2011). Development of a double antibody sandwich ELISA assay for the diagnosis of angiostrongyliasis. The Journal of Parasitology, 97(4), 721-4. https://doi.org/10.1645/GE-2590.1
Chen MX, et al. Development of a Double Antibody Sandwich ELISA Assay for the Diagnosis of Angiostrongyliasis. J Parasitol. 2011;97(4):721-4. PubMed PMID: 21506824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a double antibody sandwich ELISA assay for the diagnosis of angiostrongyliasis. AU - Chen,Mu-Xin, AU - Wang,Kun, AU - Ai,Lin, AU - Yan,Wen-Hao, AU - Peng,Liang, AU - Zhang,Ren-Li, Y1 - 2011/02/25/ PY - 2011/4/22/entrez PY - 2011/4/22/pubmed PY - 2011/9/29/medline SP - 721 EP - 4 JF - The Journal of parasitology JO - J Parasitol VL - 97 IS - 4 N2 - With the use of 2 monoclonal antibodies (MAbs) against excretory/secretory (ES) antigens of adult Angiostrongylus cantonensis, a new method was developed for double antibody sandwich ELISA for the detection of circulating antigens (CAg). To evaluate the sensitivity of the new procedure, the CAg in sera of rats (80) and mice (15) infected with A. cantonensis, as well as CAg in sera of clinically confirmed angiostrongyliasis patients (70), were evaluated. Cross-reaction testing was used to determine the specificity of serum from patients infected with Ascaris lumbricoides, Trichinella spiralis , Toxoplasma gondii , Schistosoma japonicum, Paragonimus westermani, Clonorchis sinensis, Echinococcus granulosus, Spirometra, and Taenia solium, as well as normal healthy people. The results proved that the sensitivity and the specificity of the new method were totally effective for the detection of A. cantonensis CAg. The assay is highly sensitive, specific, and reproducible, with easy handling and excellent cost effectiveness, and thereby provides a new method for the accurate diagnosis of angiostrongyliasis. SN - 1937-2345 UR - https://www.unboundmedicine.com/medline/citation/21506824/Development_of_a_double_antibody_sandwich_ELISA_assay_for_the_diagnosis_of_angiostrongyliasis_ DB - PRIME DP - Unbound Medicine ER -