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Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat.
Proc Natl Acad Sci U S A. 2011 May 10; 108(19):7727-32.PN

Abstract

Powdery mildew resistance gene Pm21, located on the chromosome 6V short arm of Haynaldia villosa and transferred to wheat as a 6VS·6AL translocation (T6VS·6AL), confers durable and broad-spectrum resistance to wheat powdery mildew. Pm21 has become a key gene resource for powdery mildew resistance breeding all over the world. In China, 12 wheat varieties containing Pm21 have been planted on more than 3.4 million hectares since 2002. Pm21 has been intractable to molecular genetic mapping because the 6VS does not pair and recombine with the 6AS. Moreover, all known accessions of H. villosa are immune to powdery mildew fungus. Pm21 is still defined by cytogenetics as a locus. In the present study, a putative serine and threonine protein kinase gene Stpk-V was cloned and characterized with an integrative strategy of molecular and cytogenetic techniques. Stpk-V is located on the Pm21 locus. The results of a single cell transient expression assay showed that Stpk-V could decrease the haustorium index dramatically. After the Stpk-V was transformed into a susceptible wheat variety Yangmai158, the characterized transgenic plants showed high and broad-spectrum powdery mildew resistance similar to T6VS·6AL. Silencing of the Stpk-V by virus-induced gene silencing in both T6VS·6AL and H. villosa resulted in their increased susceptibility. Stpk-V could be induced by Bgt and exogenous H(2)O(2), but it also mediated the increase of endogenous H(2)O(2), leading to cell death and plant resistance when the plant was attacked by Bgt.

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Authors+Show Affiliations

Cao A
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Xing L
No affiliation info available
Wang X
No affiliation info available
Yang X
No affiliation info available
Wang W
No affiliation info available
Sun Y
No affiliation info available
Qian C
No affiliation info available
Ni J
No affiliation info available
Chen Y
No affiliation info available
Liu D
No affiliation info available
Wang X
No affiliation info available
Chen P
No affiliation info available

MeSH

AscomycotaCell DeathChromosome MappingChromosomes, PlantGene Expression Regulation, PlantGene SilencingGenes, PlantHydrogen PeroxideIn Situ Hybridization, FluorescenceMolecular Sequence DataPlant DiseasesPlant ProteinsPlants, Genetically ModifiedPoaceaeProtein-Serine-Threonine KinasesTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21508323

Citation

Cao, Aizhong, et al. "Serine/threonine Kinase Gene Stpk-V, a Key Member of Powdery Mildew Resistance Gene Pm21, Confers Powdery Mildew Resistance in Wheat." Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 19, 2011, pp. 7727-32.
Cao A, Xing L, Wang X, et al. Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci U S A. 2011;108(19):7727-32.
Cao, A., Xing, L., Wang, X., Yang, X., Wang, W., Sun, Y., Qian, C., Ni, J., Chen, Y., Liu, D., Wang, X., & Chen, P. (2011). Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proceedings of the National Academy of Sciences of the United States of America, 108(19), 7727-32. https://doi.org/10.1073/pnas.1016981108
Cao A, et al. Serine/threonine Kinase Gene Stpk-V, a Key Member of Powdery Mildew Resistance Gene Pm21, Confers Powdery Mildew Resistance in Wheat. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7727-32. PubMed PMID: 21508323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. AU - Cao,Aizhong, AU - Xing,Liping, AU - Wang,Xiaoyun, AU - Yang,Xueming, AU - Wang,Wei, AU - Sun,Yulei, AU - Qian,Chen, AU - Ni,Jinlong, AU - Chen,Yaping, AU - Liu,Dajun, AU - Wang,Xiue, AU - Chen,Peidu, Y1 - 2011/04/20/ PY - 2011/4/22/entrez PY - 2011/4/22/pubmed PY - 2011/7/16/medline SP - 7727 EP - 32 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 108 IS - 19 N2 - Powdery mildew resistance gene Pm21, located on the chromosome 6V short arm of Haynaldia villosa and transferred to wheat as a 6VS·6AL translocation (T6VS·6AL), confers durable and broad-spectrum resistance to wheat powdery mildew. Pm21 has become a key gene resource for powdery mildew resistance breeding all over the world. In China, 12 wheat varieties containing Pm21 have been planted on more than 3.4 million hectares since 2002. Pm21 has been intractable to molecular genetic mapping because the 6VS does not pair and recombine with the 6AS. Moreover, all known accessions of H. villosa are immune to powdery mildew fungus. Pm21 is still defined by cytogenetics as a locus. In the present study, a putative serine and threonine protein kinase gene Stpk-V was cloned and characterized with an integrative strategy of molecular and cytogenetic techniques. Stpk-V is located on the Pm21 locus. The results of a single cell transient expression assay showed that Stpk-V could decrease the haustorium index dramatically. After the Stpk-V was transformed into a susceptible wheat variety Yangmai158, the characterized transgenic plants showed high and broad-spectrum powdery mildew resistance similar to T6VS·6AL. Silencing of the Stpk-V by virus-induced gene silencing in both T6VS·6AL and H. villosa resulted in their increased susceptibility. Stpk-V could be induced by Bgt and exogenous H(2)O(2), but it also mediated the increase of endogenous H(2)O(2), leading to cell death and plant resistance when the plant was attacked by Bgt. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/21508323/Serine/threonine_kinase_gene_Stpk_V_a_key_member_of_powdery_mildew_resistance_gene_Pm21_confers_powdery_mildew_resistance_in_wheat_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=21508323 DB - PRIME DP - Unbound Medicine ER -