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Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms.
FEBS Lett. 2011 May 06; 585(9):1293-8.FL

Abstract

Heat shock transcription factor (HSF), an evolutionarily conserved heat-responsive regulator, binds to heat shock elements (HSEs) comprising continuous inverted repeats of the pentamer nGAAn. Here, we analyzed DNA-binding ability, particularly for the discontinuously arranged nGAAn units, of HSFs from various organisms, including Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio. Among these, only C. elegans HSF failed to bind the discontinuous nGAAn units, which suggests that the target genes of HSFs in many, but not all, organisms contain discontinuous HSEs, as well as continuous HSEs. Further analysis of alternatively spliced HSF isoforms showed the importance of the C-terminal region in HSF-HSE interaction.

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Authors+Show Affiliations

Enoki Y
Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan.
Sakurai H
No affiliation info available

MeSH

Amino Acid SequenceAnimalsArabidopsisBase SequenceBinding SitesBinding, CompetitiveCaenorhabditis elegansDNADNA-Binding ProteinsDrosophila melanogasterElectrophoretic Mobility Shift AssayHeLa CellsHeat Shock Transcription FactorsHeat-Shock ResponseHumansImmunoblottingMolecular Sequence DataProtein BindingRepetitive Sequences, Nucleic AcidResponse ElementsSequence Homology, Amino AcidTranscription FactorsZebrafish

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21510947

Citation

Enoki, Yasuaki, and Hiroshi Sakurai. "Diversity in DNA Recognition By Heat Shock Transcription Factors (HSFs) From Model Organisms." FEBS Letters, vol. 585, no. 9, 2011, pp. 1293-8.
Enoki Y, Sakurai H. Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms. FEBS Lett. 2011;585(9):1293-8.
Enoki, Y., & Sakurai, H. (2011). Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms. FEBS Letters, 585(9), 1293-8. https://doi.org/10.1016/j.febslet.2011.04.014
Enoki Y, Sakurai H. Diversity in DNA Recognition By Heat Shock Transcription Factors (HSFs) From Model Organisms. FEBS Lett. 2011 May 6;585(9):1293-8. PubMed PMID: 21510947.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity in DNA recognition by heat shock transcription factors (HSFs) from model organisms. AU - Enoki,Yasuaki, AU - Sakurai,Hiroshi, Y1 - 2011/04/14/ PY - 2011/02/10/received PY - 2011/03/30/revised PY - 2011/04/05/accepted PY - 2011/4/23/entrez PY - 2011/4/23/pubmed PY - 2011/8/4/medline SP - 1293 EP - 8 JF - FEBS letters JO - FEBS Lett VL - 585 IS - 9 N2 - Heat shock transcription factor (HSF), an evolutionarily conserved heat-responsive regulator, binds to heat shock elements (HSEs) comprising continuous inverted repeats of the pentamer nGAAn. Here, we analyzed DNA-binding ability, particularly for the discontinuously arranged nGAAn units, of HSFs from various organisms, including Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio. Among these, only C. elegans HSF failed to bind the discontinuous nGAAn units, which suggests that the target genes of HSFs in many, but not all, organisms contain discontinuous HSEs, as well as continuous HSEs. Further analysis of alternatively spliced HSF isoforms showed the importance of the C-terminal region in HSF-HSE interaction. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/21510947/Diversity_in_DNA_recognition_by_heat_shock_transcription_factors__HSFs__from_model_organisms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(11)00261-4 DB - PRIME DP - Unbound Medicine ER -