Tags

Type your tag names separated by a space and hit enter

Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: epidemiologic and molecular characteristics of methicillin-resistant S. aureus clones.
Diagn Microbiol Infect Dis 2011; 70(1):22-30DM

Abstract

Between May 2008 and October 2009, a total of 2103 interns were randomly tested for nasal colonization of S. aureus and methicillin-resistant Staphylococcus aureus (MRSA). The prevalence of S. aureus among staphylococci specimens was 23.1%, and among the total S. aureus the MRSA prevalence was 9.4%. MRSA isolates were further subtyped using genetic element staphylococcal cassette chromosome mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE) band pattern, and multilocus sequence typing. SCCmec type IVa was the most prevalent strain, at 45.4 %. Eleven PFGE patterns were identified in MRSA strains, with 1 predominant (pulsotype A, 45.5%). Eight strains which belonged to clonal complex 78 carried type IVa SCCmec and produced type 3 coagulase. Panton-Valentine leukocidin (PVL) genes (lukS and F-PV) were identified in 10 (45.4%) MRSA strains; these predominately carried ϕSa2958type and ϕSa108PVL-like type PVL phages. After inducing prophages, 8 strains infected other S. aureus isolates and could generate novel PVL-positive strains of S. aureus. The present study demonstrates that interns can carry certain MRSA strains asymptomatically and contribute to the spread of MRSA between the community and hospital.

Authors+Show Affiliations

Department of Medical Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, People's Republic of China. xxma@mail.cmu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21513841

Citation

Ma, Xiao Xue, et al. "Nasal Carriage of Methicillin-resistant Staphylococcus Aureus Among Preclinical Medical Students: Epidemiologic and Molecular Characteristics of Methicillin-resistant S. Aureus Clones." Diagnostic Microbiology and Infectious Disease, vol. 70, no. 1, 2011, pp. 22-30.
Ma XX, Sun DD, Wang S, et al. Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: epidemiologic and molecular characteristics of methicillin-resistant S. aureus clones. Diagn Microbiol Infect Dis. 2011;70(1):22-30.
Ma, X. X., Sun, D. D., Wang, S., Wang, M. L., Li, M., Shang, H., ... Luo, E. J. (2011). Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: epidemiologic and molecular characteristics of methicillin-resistant S. aureus clones. Diagnostic Microbiology and Infectious Disease, 70(1), pp. 22-30. doi:10.1016/j.diagmicrobio.2010.12.004.
Ma XX, et al. Nasal Carriage of Methicillin-resistant Staphylococcus Aureus Among Preclinical Medical Students: Epidemiologic and Molecular Characteristics of Methicillin-resistant S. Aureus Clones. Diagn Microbiol Infect Dis. 2011;70(1):22-30. PubMed PMID: 21513841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: epidemiologic and molecular characteristics of methicillin-resistant S. aureus clones. AU - Ma,Xiao Xue, AU - Sun,Dan Dan, AU - Wang,Si, AU - Wang,Mei Lian, AU - Li,Miao, AU - Shang,Hong, AU - Wang,En Hua, AU - Luo,En Jie, PY - 2010/09/01/received PY - 2010/12/03/revised PY - 2010/12/04/accepted PY - 2011/4/26/entrez PY - 2011/4/26/pubmed PY - 2011/8/4/medline SP - 22 EP - 30 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 70 IS - 1 N2 - Between May 2008 and October 2009, a total of 2103 interns were randomly tested for nasal colonization of S. aureus and methicillin-resistant Staphylococcus aureus (MRSA). The prevalence of S. aureus among staphylococci specimens was 23.1%, and among the total S. aureus the MRSA prevalence was 9.4%. MRSA isolates were further subtyped using genetic element staphylococcal cassette chromosome mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE) band pattern, and multilocus sequence typing. SCCmec type IVa was the most prevalent strain, at 45.4 %. Eleven PFGE patterns were identified in MRSA strains, with 1 predominant (pulsotype A, 45.5%). Eight strains which belonged to clonal complex 78 carried type IVa SCCmec and produced type 3 coagulase. Panton-Valentine leukocidin (PVL) genes (lukS and F-PV) were identified in 10 (45.4%) MRSA strains; these predominately carried ϕSa2958type and ϕSa108PVL-like type PVL phages. After inducing prophages, 8 strains infected other S. aureus isolates and could generate novel PVL-positive strains of S. aureus. The present study demonstrates that interns can carry certain MRSA strains asymptomatically and contribute to the spread of MRSA between the community and hospital. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/21513841/Nasal_carriage_of_methicillin_resistant_Staphylococcus_aureus_among_preclinical_medical_students:_epidemiologic_and_molecular_characteristics_of_methicillin_resistant_S__aureus_clones_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(10)00557-2 DB - PRIME DP - Unbound Medicine ER -