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Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro.
Peptides. 2011 Jun; 32(6):1244-8.P

Abstract

Neuregulin-1β (NRG-1β) signaling has multiple functions in neurons. To assess NRG-1β on neurite outgrowth and neuronal migration in vitro, organotypic dorsal root ganglion (DRG) neuronal culture model was established. Neurite outgrowth and neuronal migration were evaluated using this culture model in the presence (5nmol/L, 10nmol/L, 20nmol/L) or absence of NRG-1β. Neurofilament 200 (NF-200)-immunoreactive (IR) neurons were determined as the migrating neurons. The number of nerve fiber bundles extended from DRG explant increased significantly in the presence of NRG-1β (5nmol/L, 23.0±2.2, P<0.05; 10nmol/L, 27.0±2.7, P<0.001; 20nmol/L, 30.8±3.7, P<0.001) as compared with that in the absence of NRG-1β (19.0±2.2). The number of neurons migrating from DRG explants increased significantly in the presence of NRG-1β (5nmol/L, 39.6±5.0, P<0.05; 10nmol/L, 54.6±6.7, P<0.001; 20nmol/L, 62.2±5.7, P<0.001) as compared with that in the absence of NRG-1β (31.6±4.0). Moreover, the increase of the number of nerve fiber bundles and the number of migrating NF-200-IR neurons was dose-dependent for NRG-1β addition. The data in this study imply that NRG-1β promotes neurite outgrowth and neuronal migration from DRG explants in vitro.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, 44 Wenhua Xi Road, Jinan, Shandong Province 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21515322

Citation

Liu, Zhen, et al. "Neuregulin-1β Regulates Outgrowth of Neurites and Migration of Neurofilament 200 Neurons From Dorsal Root Ganglial Explants in Vitro." Peptides, vol. 32, no. 6, 2011, pp. 1244-8.
Liu Z, Gao W, Wang Y, et al. Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro. Peptides. 2011;32(6):1244-8.
Liu, Z., Gao, W., Wang, Y., Zhang, W., Liu, H., & Li, Z. (2011). Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro. Peptides, 32(6), 1244-8. https://doi.org/10.1016/j.peptides.2011.04.005
Liu Z, et al. Neuregulin-1β Regulates Outgrowth of Neurites and Migration of Neurofilament 200 Neurons From Dorsal Root Ganglial Explants in Vitro. Peptides. 2011;32(6):1244-8. PubMed PMID: 21515322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuregulin-1β regulates outgrowth of neurites and migration of neurofilament 200 neurons from dorsal root ganglial explants in vitro. AU - Liu,Zhen, AU - Gao,Wei, AU - Wang,Yanping, AU - Zhang,Weiwei, AU - Liu,Huaxiang, AU - Li,Zhenzhong, Y1 - 2011/04/15/ PY - 2011/03/05/received PY - 2011/04/04/revised PY - 2011/04/07/accepted PY - 2011/4/26/entrez PY - 2011/4/26/pubmed PY - 2011/9/29/medline SP - 1244 EP - 8 JF - Peptides JO - Peptides VL - 32 IS - 6 N2 - Neuregulin-1β (NRG-1β) signaling has multiple functions in neurons. To assess NRG-1β on neurite outgrowth and neuronal migration in vitro, organotypic dorsal root ganglion (DRG) neuronal culture model was established. Neurite outgrowth and neuronal migration were evaluated using this culture model in the presence (5nmol/L, 10nmol/L, 20nmol/L) or absence of NRG-1β. Neurofilament 200 (NF-200)-immunoreactive (IR) neurons were determined as the migrating neurons. The number of nerve fiber bundles extended from DRG explant increased significantly in the presence of NRG-1β (5nmol/L, 23.0±2.2, P<0.05; 10nmol/L, 27.0±2.7, P<0.001; 20nmol/L, 30.8±3.7, P<0.001) as compared with that in the absence of NRG-1β (19.0±2.2). The number of neurons migrating from DRG explants increased significantly in the presence of NRG-1β (5nmol/L, 39.6±5.0, P<0.05; 10nmol/L, 54.6±6.7, P<0.001; 20nmol/L, 62.2±5.7, P<0.001) as compared with that in the absence of NRG-1β (31.6±4.0). Moreover, the increase of the number of nerve fiber bundles and the number of migrating NF-200-IR neurons was dose-dependent for NRG-1β addition. The data in this study imply that NRG-1β promotes neurite outgrowth and neuronal migration from DRG explants in vitro. SN - 1873-5169 UR - https://www.unboundmedicine.com/medline/citation/21515322/Neuregulin_1β_regulates_outgrowth_of_neurites_and_migration_of_neurofilament_200_neurons_from_dorsal_root_ganglial_explants_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(11)00153-7 DB - PRIME DP - Unbound Medicine ER -