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Inhibitory effects of Physalis angulata on tumor metastasis and angiogenesis.
J Ethnopharmacol. 2011 Jun 01; 135(3):762-71.JE

Abstract

ETHNOPHARMACOLOGICAL RELAVENCE: Physalis angulata is well-known in traditional Chinese medicine as a ingredient for various herbal formulation; also, it has been shown to exhibit anti-cancer and anti-inflammatory effects. In this study, the ability of P. angulata to inhibit tumor metastasis and angiogenesis was investigated.

MATERIALS AND METHODS

Anti-proliferative activity of ethyl acetate extracts of P. angulata (PA extracts), was determined against human oral squamous carcinoma (HSC-3) and human umbilical vein endothelial cells (HUVECs) by trypan blue exclusion method. Wound-healing migration, trans-well invasion, Western blotting and chick chorioallantoic membrane assay were carried out to determine the anti-metastatic and anti-angiogenic effects of PA extracts in vitro and in vivo.

RESULTS

We demonstrated that at sub-cytotoxic concentrations of PA extracts (5-15 μg/mL) markedly inhibited the migration and invasion of highly metastatic HSC-3 cells as shown by wound-healing repair assay and trans-well assay. Gelatin zymography assay showed that PA extracts suppressed the activity of matrix metalloproteinase (MMP)-9 and -2, and urokinase plasminogen activator (u-PA) in HSC-3 cells. In addition, Western blot analysis confirmed that PA extracts significantly decreased MMP-2 and u-PA protein expression in HSC-3 cells. Notably, PA extracts significantly augmented the expression of their endogenous inhibitors, including tissue inhibitors of MMP (TIMP-1 and -2), and plasminogen activator inhibitors (PAI-1 and -2). Further investigations revealed that non-cytotoxic concentration of PA extracts (5-15 μg/mL) inhibited vascular endothelial growth factor (VEGF)-induced proliferation, and migration/invasion of HUVECs in vitro. PA extracts also suppressed the activity of MMP-9, but not MMP-2, in HUVECs. Further, we observed, PA extracts strongly suppressed neovessel formation in the chorioallantoic membrane of chick embryos in vivo.

CONCLUSIONS

These results strongly support an anti-metastatic and anti-angiogenic activity of P. angulata that may contribute to the development of better chemopreventive agent for cancer and inflammation.

Authors+Show Affiliations

Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21515352

Citation

Hseu, You-Cheng, et al. "Inhibitory Effects of Physalis Angulata On Tumor Metastasis and Angiogenesis." Journal of Ethnopharmacology, vol. 135, no. 3, 2011, pp. 762-71.
Hseu YC, Wu CR, Chang HW, et al. Inhibitory effects of Physalis angulata on tumor metastasis and angiogenesis. J Ethnopharmacol. 2011;135(3):762-71.
Hseu, Y. C., Wu, C. R., Chang, H. W., Kumar, K. J., Lin, M. K., Chen, C. S., Cho, H. J., Huang, C. Y., Huang, C. Y., Lee, H. Z., Hsieh, W. T., Chung, J. G., Wang, H. M., & Yang, H. L. (2011). Inhibitory effects of Physalis angulata on tumor metastasis and angiogenesis. Journal of Ethnopharmacology, 135(3), 762-71. https://doi.org/10.1016/j.jep.2011.04.016
Hseu YC, et al. Inhibitory Effects of Physalis Angulata On Tumor Metastasis and Angiogenesis. J Ethnopharmacol. 2011 Jun 1;135(3):762-71. PubMed PMID: 21515352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of Physalis angulata on tumor metastasis and angiogenesis. AU - Hseu,You-Cheng, AU - Wu,Chi-Rei, AU - Chang,Hsueh-Wei, AU - Kumar,K J Senthil, AU - Lin,Ming-Kuem, AU - Chen,Chih-Sheng, AU - Cho,Hsin-Ju, AU - Huang,Chun-Yin, AU - Huang,Chih-Yang, AU - Lee,Hong-Zin, AU - Hsieh,Wen-Tsong, AU - Chung,Jing-Gung, AU - Wang,Hui-Min, AU - Yang,Hsin-Ling, Y1 - 2011/04/14/ PY - 2011/01/27/received PY - 2011/03/11/revised PY - 2011/04/08/accepted PY - 2011/4/26/entrez PY - 2011/4/26/pubmed PY - 2011/12/30/medline SP - 762 EP - 71 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 135 IS - 3 N2 - UNLABELLED: ETHNOPHARMACOLOGICAL RELAVENCE: Physalis angulata is well-known in traditional Chinese medicine as a ingredient for various herbal formulation; also, it has been shown to exhibit anti-cancer and anti-inflammatory effects. In this study, the ability of P. angulata to inhibit tumor metastasis and angiogenesis was investigated. MATERIALS AND METHODS: Anti-proliferative activity of ethyl acetate extracts of P. angulata (PA extracts), was determined against human oral squamous carcinoma (HSC-3) and human umbilical vein endothelial cells (HUVECs) by trypan blue exclusion method. Wound-healing migration, trans-well invasion, Western blotting and chick chorioallantoic membrane assay were carried out to determine the anti-metastatic and anti-angiogenic effects of PA extracts in vitro and in vivo. RESULTS: We demonstrated that at sub-cytotoxic concentrations of PA extracts (5-15 μg/mL) markedly inhibited the migration and invasion of highly metastatic HSC-3 cells as shown by wound-healing repair assay and trans-well assay. Gelatin zymography assay showed that PA extracts suppressed the activity of matrix metalloproteinase (MMP)-9 and -2, and urokinase plasminogen activator (u-PA) in HSC-3 cells. In addition, Western blot analysis confirmed that PA extracts significantly decreased MMP-2 and u-PA protein expression in HSC-3 cells. Notably, PA extracts significantly augmented the expression of their endogenous inhibitors, including tissue inhibitors of MMP (TIMP-1 and -2), and plasminogen activator inhibitors (PAI-1 and -2). Further investigations revealed that non-cytotoxic concentration of PA extracts (5-15 μg/mL) inhibited vascular endothelial growth factor (VEGF)-induced proliferation, and migration/invasion of HUVECs in vitro. PA extracts also suppressed the activity of MMP-9, but not MMP-2, in HUVECs. Further, we observed, PA extracts strongly suppressed neovessel formation in the chorioallantoic membrane of chick embryos in vivo. CONCLUSIONS: These results strongly support an anti-metastatic and anti-angiogenic activity of P. angulata that may contribute to the development of better chemopreventive agent for cancer and inflammation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21515352/Inhibitory_effects_of_Physalis_angulata_on_tumor_metastasis_and_angiogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00256-X DB - PRIME DP - Unbound Medicine ER -