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Disparities in TLR5 expression and responsiveness to flagellin in equine neutrophils and mononuclear phagocytes.
J Immunol. 2011 Jun 01; 186(11):6263-70.JI

Abstract

As sentinel cells of the innate immune system, neutrophils and mononuclear phagocytes use specific TLRs to recognize the conserved molecular patterns that characterize microbes. This study was performed to compare the responses of equine neutrophils and mononuclear phagocytes to LPS and flagellin, components of bacteria that are recognized by TLR4 and TLR5, respectively. Neutrophils and mononuclear phagocytes isolated from healthy horses were incubated in vitro with LPS, flagellin, or pronase-inactivated flagellin in the presence or absence of polymyxin B. Production of reactive oxygen species and expression of mRNA for proinflammatory cytokines were used as readouts for activation of neutrophils; production of TNF-α was used for the mononuclear cells. Western blot analysis and flow cytometry were used to detect TLR5 protein in both cell types. Although the neutrophils responded to both LPS and flagellin by producing reactive oxygen species and expressing mRNA for proinflammatory cytokines, flagellin had no stimulatory effect on monocytes or macrophages. Although both neutrophils and monocytes expressed mRNA for TLR5, it appeared to be translated into protein only by the neutrophils. Incubation with neither LPS nor IFN-γ altered TLR5 expression by the monocytes. These findings indicate that flagellin has disparate effects on neutrophils and mononuclear phagocytes isolated from horses, a species that is exquisitely sensitive to the TLR4 ligand, LPS, and that equine mononuclear phagocytes, unlike corresponding cells of other mammalian species, lack surface expression of TLR5 and do not respond to flagellin.

Authors+Show Affiliations

Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21518971

Citation

Kwon, Soyoung, et al. "Disparities in TLR5 Expression and Responsiveness to Flagellin in Equine Neutrophils and Mononuclear Phagocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 186, no. 11, 2011, pp. 6263-70.
Kwon S, Gewirtz AT, Hurley DJ, et al. Disparities in TLR5 expression and responsiveness to flagellin in equine neutrophils and mononuclear phagocytes. J Immunol. 2011;186(11):6263-70.
Kwon, S., Gewirtz, A. T., Hurley, D. J., Robertson, T. P., Moore, J. N., & Vandenplas, M. L. (2011). Disparities in TLR5 expression and responsiveness to flagellin in equine neutrophils and mononuclear phagocytes. Journal of Immunology (Baltimore, Md. : 1950), 186(11), 6263-70. https://doi.org/10.4049/jimmunol.1003824
Kwon S, et al. Disparities in TLR5 Expression and Responsiveness to Flagellin in Equine Neutrophils and Mononuclear Phagocytes. J Immunol. 2011 Jun 1;186(11):6263-70. PubMed PMID: 21518971.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Disparities in TLR5 expression and responsiveness to flagellin in equine neutrophils and mononuclear phagocytes. AU - Kwon,Soyoung, AU - Gewirtz,Andrew T, AU - Hurley,David J, AU - Robertson,Thomas P, AU - Moore,James N, AU - Vandenplas,Michel L, Y1 - 2011/04/25/ PY - 2011/4/27/entrez PY - 2011/4/27/pubmed PY - 2011/8/13/medline SP - 6263 EP - 70 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 186 IS - 11 N2 - As sentinel cells of the innate immune system, neutrophils and mononuclear phagocytes use specific TLRs to recognize the conserved molecular patterns that characterize microbes. This study was performed to compare the responses of equine neutrophils and mononuclear phagocytes to LPS and flagellin, components of bacteria that are recognized by TLR4 and TLR5, respectively. Neutrophils and mononuclear phagocytes isolated from healthy horses were incubated in vitro with LPS, flagellin, or pronase-inactivated flagellin in the presence or absence of polymyxin B. Production of reactive oxygen species and expression of mRNA for proinflammatory cytokines were used as readouts for activation of neutrophils; production of TNF-α was used for the mononuclear cells. Western blot analysis and flow cytometry were used to detect TLR5 protein in both cell types. Although the neutrophils responded to both LPS and flagellin by producing reactive oxygen species and expressing mRNA for proinflammatory cytokines, flagellin had no stimulatory effect on monocytes or macrophages. Although both neutrophils and monocytes expressed mRNA for TLR5, it appeared to be translated into protein only by the neutrophils. Incubation with neither LPS nor IFN-γ altered TLR5 expression by the monocytes. These findings indicate that flagellin has disparate effects on neutrophils and mononuclear phagocytes isolated from horses, a species that is exquisitely sensitive to the TLR4 ligand, LPS, and that equine mononuclear phagocytes, unlike corresponding cells of other mammalian species, lack surface expression of TLR5 and do not respond to flagellin. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/21518971/Disparities_in_TLR5_expression_and_responsiveness_to_flagellin_in_equine_neutrophils_and_mononuclear_phagocytes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=21518971 DB - PRIME DP - Unbound Medicine ER -