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Molecular typing and in vitro fluconazole susceptibility of Candida species isolated from diabetic and nondiabetic women with vulvovaginal candidiasis in India.
J Microbiol Immunol Infect. 2011 Jun; 44(3):166-71.JM

Abstract

BACKGROUND

Candida albicans and Candida glabrata are the major causes of vulvovaginal candidiasis (VVC) in Indian women with diabetes mellitus. Little information is available regarding the genotyping of Candida species isolated from Indian diabetic women with VVC.

METHODS

In this study, a total of 57 Candida species, comprising Candida albicans and Candida glabrata, isolated from Indian women with VVC, were genotyped and tested for fluconazole susceptibility. Arbitrarily primed polymerase chain reaction (AP-PCR) was used to genotype C glabrata isolates, whereas Southern blot hybridization using a Candida albicans repetitive element-2 (CARE-2) probe was used to genotype C albicans.

RESULTS

Genotyping showed that all the C albicans isolates were genetically heterogenous. The pattern of DNA bands obtained after AP-PCR for C glabrata strains were predominantly conformed to genotype A. In vitro fluconazole-susceptibility testing of the isolates using the Clinical and Laboratory Standards Institute M27A2 protocol showed that more than 93% of the Candida isolates were susceptible.

CONCLUSIONS

Ninety-five percent of the C albicans isolates analyzed were different and genetically unrelated. The analysis of the AP-PCR DNA banding pattern of C glabrata isolates showed that it resembled genotype "A". The Candida isolates were found to be susceptible to fluconazole, with minimum inhibitory concentrations ranging from 0.5 μg/mL to 8 μg/mL. This correlates with the use of fluconazole as a first-choice antifungal for treating VVC in India.

Authors+Show Affiliations

Department of Dermatology, School of Medicine, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44106, USA. ala16@case.edu.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21524609

Citation

Lattif, Ali Abdul, et al. "Molecular Typing and in Vitro Fluconazole Susceptibility of Candida Species Isolated From Diabetic and Nondiabetic Women With Vulvovaginal Candidiasis in India." Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, vol. 44, no. 3, 2011, pp. 166-71.
Lattif AA, Mukhopadhyay G, Banerjee U, et al. Molecular typing and in vitro fluconazole susceptibility of Candida species isolated from diabetic and nondiabetic women with vulvovaginal candidiasis in India. J Microbiol Immunol Infect. 2011;44(3):166-71.
Lattif, A. A., Mukhopadhyay, G., Banerjee, U., Goswami, R., & Prasad, R. (2011). Molecular typing and in vitro fluconazole susceptibility of Candida species isolated from diabetic and nondiabetic women with vulvovaginal candidiasis in India. Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, 44(3), 166-71. https://doi.org/10.1016/j.jmii.2011.01.020
Lattif AA, et al. Molecular Typing and in Vitro Fluconazole Susceptibility of Candida Species Isolated From Diabetic and Nondiabetic Women With Vulvovaginal Candidiasis in India. J Microbiol Immunol Infect. 2011;44(3):166-71. PubMed PMID: 21524609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular typing and in vitro fluconazole susceptibility of Candida species isolated from diabetic and nondiabetic women with vulvovaginal candidiasis in India. AU - Lattif,Ali Abdul, AU - Mukhopadhyay,Gauranga, AU - Banerjee,Uma, AU - Goswami,Ravinder, AU - Prasad,Rajendra, Y1 - 2011/01/18/ PY - 2010/06/15/received PY - 2010/07/05/revised PY - 2010/08/04/accepted PY - 2011/4/29/entrez PY - 2011/4/29/pubmed PY - 2011/10/27/medline SP - 166 EP - 71 JF - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi JO - J Microbiol Immunol Infect VL - 44 IS - 3 N2 - BACKGROUND: Candida albicans and Candida glabrata are the major causes of vulvovaginal candidiasis (VVC) in Indian women with diabetes mellitus. Little information is available regarding the genotyping of Candida species isolated from Indian diabetic women with VVC. METHODS: In this study, a total of 57 Candida species, comprising Candida albicans and Candida glabrata, isolated from Indian women with VVC, were genotyped and tested for fluconazole susceptibility. Arbitrarily primed polymerase chain reaction (AP-PCR) was used to genotype C glabrata isolates, whereas Southern blot hybridization using a Candida albicans repetitive element-2 (CARE-2) probe was used to genotype C albicans. RESULTS: Genotyping showed that all the C albicans isolates were genetically heterogenous. The pattern of DNA bands obtained after AP-PCR for C glabrata strains were predominantly conformed to genotype A. In vitro fluconazole-susceptibility testing of the isolates using the Clinical and Laboratory Standards Institute M27A2 protocol showed that more than 93% of the Candida isolates were susceptible. CONCLUSIONS: Ninety-five percent of the C albicans isolates analyzed were different and genetically unrelated. The analysis of the AP-PCR DNA banding pattern of C glabrata isolates showed that it resembled genotype "A". The Candida isolates were found to be susceptible to fluconazole, with minimum inhibitory concentrations ranging from 0.5 μg/mL to 8 μg/mL. This correlates with the use of fluconazole as a first-choice antifungal for treating VVC in India. SN - 1995-9133 UR - https://www.unboundmedicine.com/medline/citation/21524609/Molecular_typing_and_in_vitro_fluconazole_susceptibility_of_Candida_species_isolated_from_diabetic_and_nondiabetic_women_with_vulvovaginal_candidiasis_in_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1684-1182(11)00034-X DB - PRIME DP - Unbound Medicine ER -